Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online December 4, 2003; 10.1104/pp.103.027466

Plant Physiology 134:246-254 (2004)
© 2004 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
134/1/246    most recent
pp.103.027466v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bate, N. J.
Right arrow Articles by Helentjaris, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bate, N. J.
Right arrow Articles by Helentjaris, T. G.
Agricola
Right arrow Articles by Bate, N. J.
Right arrow Articles by Helentjaris, T. G.
CELL BIOLOGY AND SIGNAL TRANSDUCTION

An Invertase Inhibitor from Maize Localizes to the Embryo Surrounding Region during Early Kernel Development

Nicholas J. Bate*, Xiping Niu, Yuwen Wang, Kellie S. Reimann and Timothy G. Helentjaris

Agronomic Traits, Trait and Technology Development, Pioneer Hi-Bred International, 7250 N.W. 62nd Avenue, Johnston, Iowa, 50131-0552

Invertase activity is thought to play a regulatory role during early kernel development by converting sucrose originating from source leaves into hexoses to support cell division in the endosperm and embryo. Invertases are regulated at the posttranslational level by small protein inhibitors, INVINHs. We found that in maize (Zea mays), an invertase inhibitor homolog (ZM-INVINH1) is expressed early in kernel development, between 4 and 7 d after pollination. Invertase activity is reduced in vitro in the presence of recombinant ZM-INVINH1, and inhibition is attenuated by pre-incubation with sucrose. The presence of a putative signal peptide, fractionation experiments, and ZM-INVINH1::green fluorescent protein fusion experiments indicate that the protein is exported to the apoplast. Moreover, association of ZM-INVINH1 with the glycoprotein fraction by concanavalin A chromatogaphy suggests that ZM-INVINH1 interacts with an apoplastic invertase during early kernel development. ZM-INVINH1 was localized to the embryo surrounding region by in situ analysis, suggesting that this region forms a boundary, compartmentalizing apoplast invertase activity to allow different embryo and endosperm developmental rates.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.027466.

* Corresponding author; e-mail nic.bate{at}pioneer.com; fax 515-334-4788.

Received May 27, 2003; returned for revision August 12, 2003; accepted October 10, 2003.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
P. A. Sabelli and B. A. Larkins
The Development of Endosperm in Grasses
Plant Physiology, January 1, 2009; 149(1): 14 - 26.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. R. Morley-Smith, M. J. Pike, K. Findlay, W. Kockenberger, L. M. Hill, A. M. Smith, and S. Rawsthorne
The Transport of Sugars to Developing Embryos Is Not via the Bulk Endosperm in Oilseed Rape Seeds
Plant Physiology, August 1, 2008; 147(4): 2121 - 2130.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
G. CONSONNI, G. GAVAZZI, and S. DOLFINI
Genetic Analysis as a Tool to Investigate the Molecular Mechanisms Underlying Seed Development in Maize
Ann. Bot., September 1, 2005; 96(3): 353 - 362.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Hajduch, A. Ganapathy, J. W. Stein, and J. J. Thelen
A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database
Plant Physiology, April 1, 2005; 137(4): 1397 - 1419.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists