Plant Physiology 134:482-491 (2004)
© 2004 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
A Novel Family of Transporters Mediating the Transport of Glutathione Derivatives in Plants1
Ming-Yong Zhang2,
Andrée Bourbouloux,
Olivier Cagnac,
Chittur V. Srikanth,
Doris Rentsch,
Anand K. Bachhawat and
Serge Delrot*
Unité Mixte de Recherche Centre National de la Recherche Scientifique 6161, Transport des Assimilats, Laboratoire de Physiologie, Biochimie et Biologie Moléculaires Végétales, Bâtiment Botanique, Unité de Formation et de Recherche Sciences, 40 Avenue du Recteur Pineau, 86022 Poitiers cedex, France (M.-Y.Z., A.B., O.C., S.D.); Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India (C.V.S., A.K.B.); and Institute of Plant Sciences, Altenbergrain 21, 3013 Bern, Switzerland (D.R.)
Uptake and compartmentation of reduced glutathione (GSH), oxidized glutathione (GSSG), and glutathione conjugates are important for many functions including sulfur transport, resistance against biotic and abiotic stresses, and developmental processes. Complementation of a yeast (Saccharomyces cerevisiae) mutant (hgt1) deficient in glutathione transport was used to characterize a glutathione transporter cDNA (OsGT1) from rice (Oryza sativa). The 2.58-kb full-length cDNA (AF393848, gi 27497095), which was obtained by screening of a cDNA library and 5'-rapid amplification of cDNA ends-polymerase chain reaction, contains an open reading frame encoding a 766-amino acid protein. Complementation of the hgt1 yeast mutant strain with the OsGT1 cDNA restored growth on a medium containing GSH as the sole sulfur source. The strain expressing OsGT1 mediated [3H]GSH uptake, and this uptake was significantly competed not only by unlabeled GSSG and GS conjugates but also by some amino acids and peptides, suggesting a wide substrate specificity. OsGT1 may be involved in the retrieval of GSSG, GS conjugates, and nitrogen-containing peptides from the cell wall.
1 This work was supported by grants from the Indo-French Centre for the Promotion of Advanced Research and the Association Franco-Chinoise pour la Recherche Scientifique et Technique.
2 Present address: Chinese Academy of Sciences, Plant Physiology Laboratory, Botanical Institute of South China, 510650 Leyigu Guangzhou, People's Republic of China.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.030940.
* Corresponding author; e-mail serge.delrot{at}univ-poitiers.fr; fax 33-0-5-49-45-41-86.
Received July 27, 2003;
returned for revision September 10, 2003;
accepted October 10, 2003.
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