Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 134:1100-1112 (2004)
© 2004 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (79)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vacca, R. A.
Right arrow Articles by De Gara, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vacca, R. A.
Right arrow Articles by De Gara, L.
Agricola
Right arrow Articles by Vacca, R. A.
Right arrow Articles by De Gara, L.
CELL BIOLOGY AND SIGNAL TRANSDUCTION

Production of Reactive Oxygen Species, Alteration of Cytosolic Ascorbate Peroxidase, and Impairment of Mitochondrial Metabolism Are Early Events in Heat Shock-Induced Programmed Cell Death in Tobacco Bright-Yellow 2 Cells1

Rosa Anna Vacca2, Maria Concetta de Pinto2, Daniela Valenti, Salvatore Passarella, Ersilia Marra* and Laura De Gara

Istituto di Biomembrane e Bioenergetica, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, I–70126 Bari, Italy (R.A.V., D.V., E.M.); Dipartimento di Biologia e Patologia Vegetale, Via Orabona 4, I–70125 Bari, Italy (M.C.d.P., L.D.G.); Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, Università del Molise, Via De Sanctis, I–86100 Campobasso, Italy (S.P.); and Centro Interdipartimentale di Ricerche Biomediche, Università Campus Biomedico, Via Longoni 83, I–00155 Roma, Italy (L.D.G.)

To gain some insight into the mechanisms by which plant cells die as a result of abiotic stress, we exposed tobacco (Nicotiana tabacum) Bright-Yellow 2 cells to heat shock and investigated cell survival as a function of time after heat shock induction. Heat treatment at 55°C triggered processes leading to programmed cell death (PCD) that was complete after 72 h. In the early phase, cells undergoing PCD showed an immediate burst in hydrogen peroxide (H2O2) and superoxide (O2·-) anion production. Consistently, death was prevented by the antioxidants ascorbate (ASC) and superoxide dismutase (SOD). Actinomycin D and cycloheximide, inhibitors of transcription and translation, respectively, also prevented cell death, but with a lower efficiency. Induction of PCD resulted in gradual oxidation of endogenous ASC; this was accompanied by a decrease in both the amount and the specific activity of the cytosolic ASC peroxidase (cAPX). A reduction in cAPX gene expression was also found in the late PCD phase. Moreover, changes of cAPX kinetic properties were found in PCD cells. Production of ROS in PCD cells was accompanied by early inhibition of glucose (Glc) oxidation, with a strong impairment of mitochondrial function as shown by an increase in cellular NAD(P)H fluorescence, and by failure of mitochondria isolated from cells undergoing PCD to generate membrane potential and to oxidize succinate in a manner controlled by ADP. Thus, we propose that in the early phase of tobacco Bright-Yellow 2 cell PCD, ROS production occurs, perhaps because of damage of the cell antioxidant system, with impairment of the mitochondrial oxidative phosphorylation.

Article, publication date, and citation information can be found at http://www.plantphysiol.org/cgi/doi/10.1104/pp.103.035956.

1 This work was supported by the Italian Ministry of Instruction, University, and Research (MIVR) and by the Programmi di Ricerca di Rilevante Interesse Nazionale ("Bioenergetica: Aspetti Genetici, Biochimici e Fisiologici" to S.P. and "Specie Reattive Dell'Ossigeno e Sistemi Antiossidanti Nella Morte Cellulare Programmata e Nell'Interazione Pianta-Patogeno" to L.D.G.).

2 These authors contributed equally to the paper.

* Corresponding author; e-mail e014em01{at}area.ba.cnr.it; fax 39–080–5443317.

Received November 7, 2003; returned for revision December 9, 2003; accepted December 16, 2003.




This article has been cited by other articles:


Home page
 J Exp BotHome page
L. Zhang, Y. Li, D. Xing, and C. Gao
Characterization of mitochondrial dynamics and subcellular localization of ROS reveal that HsfA2 alleviates oxidative damage caused by heat stress in Arabidopsis
J. Exp. Bot., May 1, 2009; 60(7): 2073 - 2091.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Zhang, R.-G. Zhou, Y.-J. Gao, S.-Z. Zheng, P. Xu, S.-Q. Zhang, and D.-Y. Sun
Molecular and Genetic Evidence for the Key Role of AtCaM3 in Heat-Shock Signal Transduction in Arabidopsis
Plant Physiology, April 1, 2009; 149(4): 1773 - 1784.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Koshiba, M. Kobayashi, and T. Matoh
Boron Nutrition of Tobacco BY-2 Cells. V. Oxidative Damage is the Major Cause of Cell Death Induced by Boron Deprivation
Plant Cell Physiol., January 1, 2009; 50(1): 26 - 36.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. N. Moschou, M. Sanmartin, A. H. Andriopoulou, E. Rojo, J. J. Sanchez-Serrano, and K. A. Roubelakis-Angelakis
Bridging the Gap between Plant and Mammalian Polyamine Catabolism: A Novel Peroxisomal Polyamine Oxidase Responsible for a Full Back-Conversion Pathway in Arabidopsis
Plant Physiology, August 1, 2008; 147(4): 1845 - 1857.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
L. Zhang and D. Xing
Methyl Jasmonate Induces Production of Reactive Oxygen Species and Alterations in Mitochondrial Dynamics that Precede Photosynthetic Dysfunction and Subsequent Cell Death
Plant Cell Physiol., July 1, 2008; 49(7): 1092 - 1111.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. K. Azad, T. Ishikawa, T. Ishikawa, Y. Sawa, and H. Shibata
Intracellular energy depletion triggers programmed cell death during petal senescence in tulip
J. Exp. Bot., May 31, 2008; (2008) ern066v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Horemans, T. Raeymaekers, K. Van Beek, A. Nowocin, R. Blust, K. Broos, A. Cuypers, J. Vangronsveld, and Y. Guisez
Dehydroascorbate uptake is impaired in the early response of Arabidopsis plant cell cultures to cadmium
J. Exp. Bot., December 1, 2007; 58(15-16): 4307 - 4317.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Dong, J. L. Bufford, Y. Oono, K. Church, M. Q. Dau, K. Michels, M. Haughton, and G. Tallman
Heat Suppresses Activation of an Auxin-Responsive Promoter in Cultured Guard Cell Protoplasts of Tree Tobacco
Plant Physiology, October 1, 2007; 145(2): 367 - 377.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y.-y. Charng, H.-c. Liu, N.-y. Liu, W.-t. Chi, C.-n. Wang, S.-h. Chang, and T.-t. Wang
A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance in Arabidopsis
Plant Physiology, January 1, 2007; 143(1): 251 - 262.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
G. MILLER and R. MITTLER
Could Heat Shock Transcription Factors Function as Hydrogen Peroxide Sensors in Plants?
Ann. Bot., August 1, 2006; 98(2): 279 - 288.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Van Breusegem and J. F. Dat
Reactive Oxygen Species in Plant Cell Death
Plant Physiology, June 1, 2006; 141(2): 384 - 390.
[Full Text] [PDF]

Home page
 J Exp BotHome page
V. Locato, A. Balestrazzi, L. De Gara, and D. Carbonera
Reduced expression of top1{beta} gene induces programmed cell death and alters ascorbate metabolism in Daucus carota cultured cells
J. Exp. Bot., May 1, 2006; 57(8): 1667 - 1676.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. A. Vacca, D. Valenti, A. Bobba, R. S. Merafina, S. Passarella, and E. Marra
Cytochrome c Is Released in a Reactive Oxygen Species-Dependent Manner and Is Degraded via Caspase-Like Proteases in Tobacco Bright-Yellow 2 Cells en Route to Heat Shock-Induced Cell Death
Plant Physiology, May 1, 2006; 141(1): 208 - 219.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F.-Q. Guo and N. M. Crawford
Arabidopsis Nitric Oxide Synthase1 Is Targeted to Mitochondria and Protects against Oxidative Damage and Dark-Induced Senescence
PLANT CELL, December 1, 2005; 17(12): 3436 - 3450.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
K. YOSHINAGA, S.-I. ARIMURA, Y. NIWA, N. TSUTSUMI, H. UCHIMIYA, and M. KAWAI-YAMADA
Mitochondrial Behaviour in the Early Stages of ROS Stress Leading to Cell Death in Arabidopsis thaliana
Ann. Bot., August 1, 2005; 96(2): 337 - 342.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Ishikawa, Y. Morimoto, R. Madhusudhan, Y. Sawa, H. Shibata, Y. Yabuta, A. Nishizawa, and S. Shigeoka
Acclimation to Diverse Environmental Stresses Caused by a Suppression of Cytosolic Ascorbate Peroxidase in Tobacco BY-2 cells
Plant Cell Physiol., August 1, 2005; 46(8): 1264 - 1271.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Larkindale, J. D. Hall, M. R. Knight, and E. Vierling
Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance
Plant Physiology, June 1, 2005; 138(2): 882 - 897.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. H. Lee, A. P. Singh, and G. C. Chung
Rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decreases in water transport
J. Exp. Bot., August 1, 2004; 55(403): 1733 - 1741.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists