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First published online February 19, 2004; 10.1104/pp.103.033936

Plant Physiology 134:1200-1205 (2004)
© 2004 American Society of Plant Biologists

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BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

myo-Inositol Oxygenase Offers a Possible Entry Point into Plant Ascorbate Biosynthesis1

Argelia Lorence, Boris I. Chevone, Pedro Mendes and Craig L. Nessler*

Department of Plant Pathology, Physiology, and Weed Science (A.L., B.I.C., C.L.N.) and Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 (P.M.)

Two biosynthetic pathways for ascorbate (L-ascorbic acid [AsA]; vitamin C) in plants are presently known, the mannose/L-galactose pathway and an L-GalUA pathway. Here, we present molecular and biochemical evidence for a possible biosynthetic route using myo-inositol (MI) as the initial substrate. A MI oxygenase (MIOX) gene was identified in chromosome 4 (miox4) of Arabidopsis ecotype Columbia, and its enzymatic activity was confirmed in bacterially expressed recombinant protein. Miox4 was primarily expressed in flowers and leaves of wild-type Arabidopsis plants, tissues with a high concentration of AsA. Ascorbate levels increased 2- to 3-fold in homozygous Arabidopsis lines overexpressing the miox4 open reading frame, thus suggesting the role of MI in AsA biosynthesis and the potential for using this gene for the agronomic and nutritional enhancement of crops.

Article, publication date, and citation information can be found at http://www.plantphysiol.org/cgi/doi/10.1104/pp.103.033936.

1 This work was supported by the Interagency Metabolic Engineering Program (National Science Foundation Metabolic Biochemistry and Integrative Plant Biology) (IBN0118612) and USDA/CREES (2002-3S321-11600).

* Corresponding author; e-mail cnessler{at}vt.edu, fax 540–231–7477.

Received September 26, 2003; returned for revision November 18, 2003; accepted December 9, 2003.




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