This Article Full Text Full Text (PDF) All Versions of this Article: 134/4/1471    most recent pp.103.037754v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (25) Citing Articles via Google Scholar Google Scholar Articles by Domonkos, I. Articles by Gombos, Z. Search for Related Content PubMed PubMed Citation Articles by Domonkos, I. Articles by Gombos, Z. Agricola Articles by Domonkos, I. Articles by Gombos, Z.

BIOENERGETICS AND PHOTOSYNTHESIS

Phosphatidylglycerol Is Essential for Oligomerization of Photosystem I Reaction Center¹

Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H–6701 Szeged, Hungary (I.D., A.S., L.K., B.U., B.B., M.K., Z.G.); Department of Plant Physiology and Biochemistry, Faculty of Biotechnology, Jagiellonian University, PL–30–387 Krakow, Poland (P.M., K.S.); Division of Material Science (Physics), Graduate School of Science, Nagoya University, Furocho, Chikusa, Nagoya 464–8601, Japan (K.I., S.I.); Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 (G.S.); Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komamba, Tokyo 153–8902, Japan (I.S., H.W.); and Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H–6701 Szeged, Hungary (T.F.)

Our earlier studies with the pgsA mutant of Synechocystis PCC6803 demonstrated the important role of phosphatidylglycerol (PG) in PSII dimer formation and in electron transport between the primary and secondary electron-accepting plastoquinones of PSII. Using a long-term depletion of PG from pgsA mutant cells, we could induce a decrease not only in PSII but also in PSI activity. Simultaneously with the decrease in PSI activity, dramatic structural changes of the PSI complex were detected. A 21-d PG depletion resulted in the degradation of PSI trimers and concomitant accumulation of monomer PSI. The analyses of PSI particles isolated by MonoQ chromatography showed that, following the 21-d depletion, PSI trimers were no longer detectable in the thylakoid membranes. Immunoblot analyses revealed that the PSI monomers accumulating in the PG-depleted mutant cells do not contain PsaL, the protein subunit thought to be responsible for the trimer formation. Nevertheless, the trimeric structure of PSI reaction center could be restored by readdition of PG, even in the presence of the protein synthesis inhibitor lincomycin, indicating that free PsaL was present in thylakoid membranes following the 21-d PG depletion. Our data suggest an indispensable role for PG in the PsaL-mediated assembly of the PSI reaction center.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.037754.

^* Corresponding author; e-mail domonkos{at}nucleus.szbk.u-szeged.hu; fax 36–62–433–434.

Received December 15, 2003; returned for revision January 27, 2004; accepted January 27, 2004.

This article has been cited by other articles:

				I. Domonkos, P. Malec, H. Laczko-Dobos, O. Sozer, K. Klodawska, H. Wada, K. Strzalka, and Z. Gombos Phosphatidylglycerol Depletion Induces an Increase in Myxoxanthophyll Biosynthetic Activity in Synechocystis PCC6803 Cells Plant Cell Physiol., February 1, 2009; 50(2): 374 - 382. [Abstract] [Full Text] [PDF]

				I. Sakurai, J.-R. Shen, J. Leng, S. Ohashi, M. Kobayashi, and H. Wada Lipids in Oxygen-Evolving Photosystem II Complexes of Cyanobacteria and Higher Plants J. Biochem., August 1, 2006; 140(2): 201 - 209. [Abstract] [Full Text] [PDF]

				A. G. Ivanov, M. Krol, D. Sveshnikov, E. Selstam, S. Sandstrom, M. Koochek, Y.-I. Park, S. Vasil'ev, D. Bruce, G. Oquist, et al. Iron Deficiency in Cyanobacteria Causes Monomerization of Photosystem I Trimers and Reduces the Capacity for State Transitions and the Effective Absorption Cross Section of Photosystem I in Vivo Plant Physiology, August 1, 2006; 141(4): 1436 - 1445. [Abstract] [Full Text] [PDF]

				F. Wu, Z. Yang, and T. Kuang Impaired Photosynthesis in Phosphatidylglycerol-Deficient Mutant of Cyanobacterium Anabaena sp. PCC7120 with a Disrupted Gene Encoding a Putative Phosphatidylglycerophosphatase Plant Physiology, August 1, 2006; 141(4): 1274 - 1283. [Abstract] [Full Text] [PDF]