First published online April 2, 2004; 10.1104/pp.103.036970
Plant Physiology 134:1697-1707 (2004)
© 2004 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
Tissue-Specific Localization of an Abscisic Acid Biosynthetic Enzyme, AAO3, in Arabidopsis1
Hanae Koiwai,
Kentaro Nakaminami2,
Mitsunori Seo,
Wataru Mitsuhashi,
Tomonobu Toyomasu and
Tomokazu Koshiba*
Department of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192–0397, Japan (H.K., T.K); Course of the Science of Bioresources, The United Graduate School of Agricultural Science, Iwate University, Morioka, Iwate 020–8550, Japan (K.N., W.M., T.T.); Plant Science Center, RIKEN (The Institute of Physical and Chemical Research), Yokohama, Kanagawa 230–0045, Japan (M.S.); and Faculty of Agriculture, Yamagata University, Wakaba-cho, Turuoka 997–8555, Japan (W.M., T.T.)
Arabidopsis aldehyde oxidase 3 (AAO3) is an enzyme involved in abscisic acid (ABA) biosynthesis in response to drought stress. Since the enzyme catalyzes the last step of the pathway, ABA production sites may be determined by the presence of AAO3. Here, AAO3 localization was investigated using AAO3 promoter:AAO3-GFP transgenic plants and by an immunohistochemical technique. AAO3-GFP protein exhibited an activity to produce ABA from abscisic aldehyde, and the transgene restored the wilty phenotype of the aao3 mutant. GFP-fluorescence was detected in the root tips, vascular bundles of roots, hypocotyls and inflorescence stems, and along the leaf veins. Intense immunofluorescence signals were localized in phloem companion cells and xylem parenchyma cells. Faint but significant GFP- and immuno-fluorescence signals were observed in the leaf guard cells. In situ hybridization with antisense AAO3 mRNA showed AAO3 mRNA expression in the guard cells of dehydrated leaves. These results indicate that the ABA synthesized in vascular systems is transported to various target tissues and cells, and also that the guard cells themselves are able to synthesize ABA.
1 This work was supported in part by the Ministry of Education, Science, Sports and Culture of Japan (Grant-in-Aid for Scientific Research (B) no. 13490024 to T.K.) and by the Japan Society for the Promotion of Science for Young Scientists (Research Fellowship to H.K.).
2 Present address: National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira-ku, Sapporo 062–8555, Japan.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.036970.
* Corresponding author; e-mail koshiba-tomokazu{at}c.metro-u.ac.jp; fax 81–426–2565.
Received December 1, 2003;
returned for revision January 29, 2004;
accepted January 30, 2004.
This article has been cited by other articles:
|
|
|
|
 
M. Ibdah, Y.-T. Chen, C. G. Wilkerson, and E. Pichersky
An Aldehyde Oxidase in Developing Seeds of Arabidopsis Converts Benzaldehyde to Benzoic Acid
Plant Physiology,
May 1, 2009;
150(1):
416 - 423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Sirichandra, A. Wasilewska, F. Vlad, C. Valon, and J. Leung
The guard cell as a single-cell model towards understanding drought tolerance and abscisic acid action
J. Exp. Bot.,
April 1, 2009;
60(5):
1439 - 1463.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Okamoto, Y. Tanaka, S. R. Abrams, Y. Kamiya, M. Seki, and E. Nambara
High Humidity Induces Abscisic Acid 8'-Hydroxylase in Stomata and Vasculature to Regulate Local and Systemic Abscisic Acid Responses in Arabidopsis
Plant Physiology,
February 1, 2009;
149(2):
825 - 834.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. O. Zubo, M. V. Yamburenko, S. Y. Selivankina, F. M. Shakirova, A. M. Avalbaev, N. V. Kudryakova, N. K. Zubkova, K. Liere, O. N. Kulaeva, V. V. Kusnetsov, et al.
Cytokinin Stimulates Chloroplast Transcription in Detached Barley Leaves
Plant Physiology,
October 1, 2008;
148(2):
1082 - 1093.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Schmitt, R. Stadler, and N. Sauer
Immunolocalization of Solanaceous SUT1 Proteins in Companion Cells and Xylem Parenchyma: New Perspectives for Phloem Loading and Transport
Plant Physiology,
September 1, 2008;
148(1):
187 - 199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Endo, Y. Sawada, H. Takahashi, M. Okamoto, K. Ikegami, H. Koiwai, M. Seo, T. Toyomasu, W. Mitsuhashi, K. Shinozaki, et al.
Drought Induction of Arabidopsis 9-cis-Epoxycarotenoid Dioxygenase Occurs in Vascular Parenchyma Cells
Plant Physiology,
August 1, 2008;
147(4):
1984 - 1993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Leegood
Roles of the bundle sheath cells in leaves of C3 plants
J. Exp. Bot.,
May 1, 2008;
59(7):
1663 - 1673.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Wasilewska, F. Vlad, C. Sirichandra, Y. Redko, F. Jammes, C. Valon, N. F. d. Frey, and J. Leung
An Update on Abscisic Acid Signaling in Plants and More ...
Mol Plant,
March 1, 2008;
1(2):
198 - 217.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Liu and M. C. Mehdy
A Nonclassical Arabinogalactan Protein Gene Highly Expressed in Vascular Tissues, AGP31, Is Transcriptionally Repressed by Methyl Jasmonic Acid in Arabidopsis
Plant Physiology,
November 1, 2007;
145(3):
863 - 874.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-C. Lin, S.-G. Hwang, A. Endo, M. Okamoto, T. Koshiba, and W.-H. Cheng
Ectopic Expression of ABSCISIC ACID 2/GLUCOSE INSENSITIVE 1 in Arabidopsis Promotes Seed Dormancy and Stress Tolerance
Plant Physiology,
February 1, 2007;
143(2):
745 - 758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Maeda, W. Song, T. L. Sage, and D. DellaPenna
Tocopherols Play a Crucial Role in Low-Temperature Adaptation and Phloem Loading in Arabidopsis
PLANT CELL,
October 1, 2006;
18(10):
2710 - 2732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Okamoto, A. Kuwahara, M. Seo, T. Kushiro, T. Asami, N. Hirai, Y. Kamiya, T. Koshiba, and E. Nambara
CYP707A1 and CYP707A2, Which Encode Abscisic Acid 8'-Hydroxylases, Are Indispensable for Proper Control of Seed Dormancy and Germination in Arabidopsis
Plant Physiology,
May 1, 2006;
141(1):
97 - 107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Christmann, T. Hoffmann, I. Teplova, E. Grill, and A. Muller
Generation of Active Pools of Abscisic Acid Revealed by In Vivo Imaging of Water-Stressed Arabidopsis
Plant Physiology,
January 1, 2005;
137(1):
209 - 219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Seo, H. Aoki, H. Koiwai, Y. Kamiya, E. Nambara, and T. Koshiba
Comparative Studies on the Arabidopsis Aldehyde Oxidase (AAO) Gene Family Revealed a Major Role of AAO3 in ABA Biosynthesis in Seeds
Plant Cell Physiol.,
November 15, 2004;
45(11):
1694 - 1703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Tallman
Are diurnal patterns of stomatal movement the result of alternating metabolism of endogenous guard cell ABA and accumulation of ABA delivered to the apoplast around guard cells by transpiration?
J. Exp. Bot.,
September 1, 2004;
55(405):
1963 - 1976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
