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PLANTS INTERACTING WITH OTHER ORGANISMS

Adenylate Gradients and Ar:O₂ Effects on Legume Nodules. II. Changes in the Subcellular Adenylate Pools¹

Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6 (H.W., D.B.L.); and Botany, School of Plant Biology, The University of Western Australia, Nedlands, Western Australia 6907, Australia (C.A.A.)

Central infected zone tissue of soybean (Glycine max L. Merr.) nodules was fractionated into separate subcellular compartments using density gradient centrifugation in nonaqueous solvents to better understand how exposure to Ar:O₂ (80:20%, v/v) atmosphere affects C and N metabolism, and to explore a potential role for adenylates in regulating O₂ diffusion. When nodules were switched from air to Ar:O₂, adenylate energy charge (AEC) in the plant cytosol rose from 0.63 ± 0.02 to 0.73 ± 0.02 within 7 min and to 0.80 ± 0.01 by 60 min. In contrast, AEC of the mitochondrial compartment of this central zone tissue remained high (0.80 ± 0.02 to 0.81 ± 0.02) following Ar treatment while that of the bacteroid compartment was unchanged, at 0.73 ± 0.02, after 7 min, but declined to 0.57 ± 0.03 after 60 min. These results were consistent with a simulation model that predicted Ar:O₂ exposure would first reduce ATP demand for ammonia assimilation and rapidly increase cytosolic AEC, before the Ar:O₂-induced decline mediated by a decrease in nodule O₂ permeability reduces bacteroid AEC. The possibility that adenylates play a key, integrating role in regulating nodule permeability to oxygen diffusion is discussed.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.038547.

^* Corresponding author; e-mail layzelld{at}biology.queensu.ca; fax 613–533–6617.

Received January 2, 2004; returned for revision February 3, 2004; accepted February 3, 2004.

This article has been cited by other articles:

				H. Wei and D. B. Layzell Adenylate-Coupled Ion Movement. A Mechanism for the Control of Nodule Permeability to O2 Diffusion Plant Physiology, May 1, 2006; 141(1): 280 - 287. [Abstract] [Full Text] [PDF]