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First published online April 30, 2004; 10.1104/pp.103.037895

Plant Physiology 135:145-151 (2004)
© 2004 American Society of Plant Biologists

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*COPPER, ELEMENTAL
*MANGANESE COMPOUNDS
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BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Copper Transport Across Pea Thylakoid Membranes

Richard Shingles*, Larry E. Wimmers1 and Richard E. McCarty

Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218–2685

The initial rate of Cu2+ movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu2+-sensitive fluorophore Phen Green SK. Cu2+ transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu2+ concentration and saturated at about 0.6 µM total Cu2+. Cu2+ uptake was maximal at a thylakoid lumen pH of 7.0. Cu2+ transport was inhibited by Zn2+ but was largely unaffected by Mn2+ and Cu+. Zn2+ inhibited Cu2+ transport to a maximum of 60%, indicating that there may be more than one transporter for copper in pea thylakoid membranes.

1 Permanent address: Department of Biological Sciences, Towson University, 8000 York Road, Towson, MD 21252.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.037895.

* Corresponding author; shingles{at}jhu.edu; fax 410–516–5213.

Received December 23, 2004; returned for revision February 20, 2004; accepted March 8, 2004.




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