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BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Contribution of Different Carbon Sources to Isoprene Biosynthesis in Poplar Leaves¹

Forschungszentrum Karlsruhe GmbH Institut für Meteorologie und Klimaforschung, Atmosphärische Umweltforschung (IMK-IFU), D–82467 Garmisch-Partenkirchen, Germany (J.-P.S.); Institut für Ionenphysik, Leopold-Franzens-Universität Innsbruck, A–6020 Innsbruck, Austria (M.G., A.W., A.H.); and Institut für Forstbotanik und Baumphysiologie, Professur für Baumphysiologie, Albert-Ludwigs-Universität Freiburg, D–79110 Freiburg i. Br., Germany (J.K., U.H., H.R.)

This study was performed to test if alternative carbon sources besides recently photosynthetically fixed CO₂ are used for isoprene formation in the leaves of young poplar (Populus x canescens) trees. In a ¹³CO₂ atmosphere under steady state conditions, only about 75% of isoprene became ¹³C labeled within minutes. A considerable part of the unlabeled carbon may be derived from xylem transported carbohydrates, as may be shown by feeding leaves with [U-¹³C]Glc. As a consequence of this treatment approximately 8% to 10% of the carbon emitted as isoprene was ¹³C labeled. In order to identify further carbon sources, poplar leaves were depleted of leaf internal carbon pools and the carbon pools were refilled with ¹³C labeled carbon by exposure to ¹³CO₂. Results from this treatment showed that about 30% of isoprene carbon became ¹³C labeled, clearly suggesting that, in addition to xylem transported carbon and CO₂, leaf internal carbon pools, e.g. starch, are used for isoprene formation. This use was even increased when net assimilation was reduced, for example by abscisic acid application. The data provide clear evidence of a dynamic exchange of carbon between different cellular precursors for isoprene biosynthesis, and an increasing importance of these alternative carbon pools under conditions of limited photosynthesis. Feeding [1,2-¹³C]Glc and [3-¹³C]Glc to leaves via the xylem suggested that alternative carbon sources are probably derived from cytosolic pyruvate/phosphoenolpyruvate equivalents and incorporated into isoprene according to the predicted cleavage of the 3-C position of pyruvate during the initial step of the plastidic deoxyxylulose-5-phosphate pathway.

² These authors contributed equally to the paper.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.037374.

^* Corresponding author; e-mail armin.hansel{at}uibk.ac.at; fax 43–512–507–2932.

Received December 8, 2003; returned for revision March 19, 2004; accepted March 23, 2004.

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