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DEVELOPMENT AND HORMONE ACTION

Plasma Membrane Depolarization Induced by Abscisic Acid in Arabidopsis Suspension Cells Involves Reduction of Proton Pumping in Addition to Anion Channel Activation, Which Are Both Ca²⁺ Dependent

Laboratoire d'Electrophysiologie des Membranes, EA 3514, Université Paris 7, 75251 Paris cedex 05, France (M.B., Z.A., A-M.P., M.M., Z.Z., D.C., O.D., F.B., J-P.R.); and Plant Molecular Signalling Laboratory, Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom (H.K.)

In Arabidopsis suspension cells a rapid plasma membrane depolarization is triggered by abscisic acid (ABA). Activation of anion channels was shown to be a component leading to this ABA-induced plasma membrane depolarization. Using experiments employing combined voltage clamping, continuous measurement of extracellular pH, we examined whether plasma membrane H⁺-ATPases could also be involved in the depolarization. We found that ABA causes simultaneously cell depolarization and medium alkalinization, the second effect being abolished when ABA is added in the presence of H⁺ pump inhibitors. Inhibition of the proton pump by ABA is thus a second component leading to the plasma membrane depolarization. The ABA-induced depolarization is therefore the result of two different processes: activation of anion channels and inhibition of H⁺-ATPases. These two processes are independent because impairing one did not suppress the depolarization. Both processes are however dependent on the [Ca²⁺]_cyt increase induced by ABA since increase in [Ca²⁺]_cyt enhanced anion channels and impaired H⁺-ATPases.

^* Corresponding author; e-mail brault{at}ccr.jussieu.fr; fax 33–1–44–27–78–13.

Received January 19, 2004; returned for revision February 6, 2004; accepted March 4, 2004.

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