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BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Gene-Specific Involvement of -Oxidation in Wound-Activated Responses in Arabidopsis¹

Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022 Valencia, Spain (M.C.C., C.M., J.L.); and Department of Biology, Unit of Plant Biology, University of Fribourg, CH–1700 Fribourg, Switzerland (A.B., J.-P.M.)

The coordinated induced expression of -oxidation genes is essential to provide the energy supply for germination and postgerminative development. However, very little is known about other functions of -oxidation in nonreserve organs. We have identified a gene-specific pattern of induced -oxidation gene expression in wounded leaves of Arabidopsis. Mechanical damage triggered the local and systemic induction of only ACX1 among acyl-coenzyme A oxidase (ACX) genes, and KAT2/PED1 among 3-ketoacyl-coenzyme A thiolase (KAT) genes in Arabidopsis. In turn, wounding induced KAT5/PKT2 only systemically. Although most of the -oxidation genes were activated by wound-related factors such as dehydration and abscisic acid, jasmonic acid (JA) induced only ACX1 and KAT5. Reduced expression of ACX1 or KAT2 genes, in transgenic plants expressing their corresponding mRNAs in antisense orientation, correlated with defective wound-activated synthesis of JA and with reduced expression of JA-responsive genes. Induced expression of JA-responsive genes by exogenous application of JA was unaffected in those transgenic plants, suggesting that ACX1 and KAT2 play a major role in driving wound-activated responses by participating in the biosynthesis of JA in wounded Arabidopsis plants.

² These authors contributed equally to the paper.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.039925.

^* Corresponding author; e-mail jleon{at}ibmcp.upv.es; fax 34–963877859.

Received January 29, 2004; returned for revision February 22, 2004; accepted February 27, 2004.

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