Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online June 1, 2004; 10.1104/pp.103.032631

Plant Physiology 135:849-858 (2004)
© 2004 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
135/2/849    most recent
pp.103.032631v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zeeman, S. C.
Right arrow Articles by Smith, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeeman, S. C.
Right arrow Articles by Smith, S. M.
Agricola
Right arrow Articles by Zeeman, S. C.
Right arrow Articles by Smith, S. M.
BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Plastidial {alpha}-Glucan Phosphorylase Is Not Required for Starch Degradation in Arabidopsis Leaves But Has a Role in the Tolerance of Abiotic Stress1

Samuel C. Zeeman*, David Thorneycroft, Nicole Schupp, Andrew Chapple, Melanie Weck, Hannah Dunstan, Pierre Haldimann, Nicole Bechtold, Alison M. Smith and Steven M. Smith

Institute of Plant Sciences, University of Bern, CH–3013 Bern, Switzerland (S.C.Z., P.H.); Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JH, United Kingdom (D.T., H.D., S.M.S.); John Innes Centre, Norwich NR4 7UH, United Kingdom (N.S., A.C., M.W., A.M.S.); and Institut National de la Recherche Agronomique, Lab Génétique et Amélioration des Plantes, F–78026 Versailles, France (N.B.)

To study the role of the plastidial {alpha}-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial {alpha}-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild type. The loss of plastidial {alpha}-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient water deficit.

1 This work was supported by the National Centre of Competence in Research (Plant Survival), National Science Foundation, Switzerland, and by the Biotechnology and Biological Science Research Council (BBSRC) of the U.K. (grant nos. D11089 and D11090 and a competitive strategic grant to the John Innes Centre). M.W. was supported by a Leonardo award from the European Union.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.032631.

* Corresponding author; e-mail sam.zeeman{at}ips.unibe.ch; fax 41–31–631–5222.

Received August 31, 2003; returned for revision March 10, 2004; accepted March 10, 2004.




This article has been cited by other articles:


Home page
 GeneticsHome page
P. Deschamps, H. Moreau, A. Z. Worden, D. Dauvillee, and S. G. Ball
Early Gene Duplication Within Chloroplastida and Its Correspondence With Relocation of Starch Metabolism to Chloroplasts
Genetics, April 1, 2008; 178(4): 2373 - 2387.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Hofmann, D. Szakasits, A. Blochl, M. Sobczak, S. Daxbock-Horvath, W. Golinowski, H. Bohlmann, and F. M.W. Grundler
Starch Serves as Carbohydrate Storage in Nematode-Induced Syncytia
Plant Physiology, January 1, 2008; 146(1): 228 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
Z. Deng, X. Zhang, W. Tang, J. A. Oses-Prieto, N. Suzuki, J. M. Gendron, H. Chen, S. Guan, R. J. Chalkley, T. K. Peterman, et al.
A Proteomics Study of Brassinosteroid Response in Arabidopsis
Mol. Cell. Proteomics, December 1, 2007; 6(12): 2058 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Edner, J. Li, T. Albrecht, S. Mahlow, M. Hejazi, H. Hussain, F. Kaplan, C. Guy, S. M. Smith, M. Steup, et al.
Glucan, Water Dikinase Activity Stimulates Breakdown of Starch Granules by Plastidial beta-Amylases
Plant Physiology, September 1, 2007; 145(1): 17 - 28.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. L. Nielsen, A. L. Hogh, and J. Emmersen
DeepSAGE--digital transcriptomics with high sensitivity, simple experimental protocol and multiplexing of samples
Nucleic Acids Res., November 14, 2006; 34(19): e133 - e133.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Lu, J. M. Steichen, J. Yao, and T. D. Sharkey
The Role of Cytosolic {alpha}-Glucan Phosphorylase in Maltose Metabolism and the Comparison of Amylomaltase in Arabidopsis and Escherichia coli
Plant Physiology, November 1, 2006; 142(3): 878 - 889.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. Alonso-Casajus, D. Dauvillee, A. M. Viale, F. J. Munoz, E. Baroja-Fernandez, M. T. Moran-Zorzano, G. Eydallin, S. Ball, and J. Pozueta-Romero
Glycogen Phosphorylase, the Product of the glgP Gene, Catalyzes Glycogen Breakdown by Removing Glucose Units from the Nonreducing Ends in Escherichia coli.
J. Bacteriol., July 1, 2006; 188(14): 5266 - 5272.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. E. Weise, S. M. Schrader, K. R. Kleinbeck, and T. D. Sharkey
Carbon Balance and Circadian Regulation of Hydrolytic and Phosphorolytic Breakdown of Transitory Starch
Plant Physiology, July 1, 2006; 141(3): 879 - 886.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Balmer, W. H. Vensel, N. Cai, W. Manieri, P. Schurmann, W. J. Hurkman, and B. B. Buchanan
A complete ferredoxin/thioredoxin system regulates fundamental processes in amyloplasts
PNAS, February 21, 2006; 103(8): 2988 - 2993.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Borland, S. Elliott, S. Patterson, T. Taybi, J. Cushman, B. Pater, and J. Barnes
Are the metabolic components of crassulacean acid metabolism up-regulated in response to an increase in oxidative burden?
J. Exp. Bot., January 1, 2006; 57(2): 319 - 328.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T.-S. Yu, S. C. Zeeman, D. Thorneycroft, D. C. Fulton, H. Dunstan, W.-L. Lue, B. Hegemann, S.-Y. Tung, T. Umemoto, A. Chapple, et al.
{alpha}-Amylase Is Not Required for Breakdown of Transitory Starch in Arabidopsis Leaves
J. Biol. Chem., March 18, 2005; 280(11): 9773 - 9779.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. M. Smith, D. C. Fulton, T. Chia, D. Thorneycroft, A. Chapple, H. Dunstan, C. Hylton, S. C. Zeeman, and A. M. Smith
Diurnal Changes in the Transcriptome Encoding Enzymes of Starch Metabolism Provide Evidence for Both Transcriptional and Posttranscriptional Regulation of Starch Metabolism in Arabidopsis Leaves
Plant Physiology, September 1, 2004; 136(1): 2687 - 2699.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists