This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 135/2/938    most recent pp.104.040253v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Ercetin, M. E. Articles by Gillaspy, G. E. Search for Related Content PubMed PubMed Citation Articles by Ercetin, M. E. Articles by Gillaspy, G. E. Agricola Articles by Ercetin, M. E. Articles by Gillaspy, G. E.

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Molecular Characterization of an Arabidopsis Gene Encoding a Phospholipid-Specific Inositol Polyphosphate 5-Phosphatase^1,[w]

Department of Biochemistry and Fralin Biotechnology Center, Virginia Tech, Blacksburg, Virginia 24061

Phosphoinositides are important molecules that serve as second messengers and bind to a complex array of proteins modulating their subcellular location and activity. The enzymes that metabolize phosphoinositides can in some cases serve to terminate the signaling actions of phosphoinositides. The inositol polyphosphate 5-phosphatases (5PTases) comprise a large protein family that hydrolyzes 5-phosphates from a variety of inositol phosphate and phosphoinositide substrates. We previously reported the identification of 15 putative 5PTase genes in Arabidopsis and have shown that overexpression of the At5PTase1 gene can alter abscisic acid signaling. At5PTase1 and At5PTase2 have been shown to hydrolyze the 5-phosphate from inositol phosphate substrates. We have examined the substrate specificity of the At5PTase11 protein, which is one of the smallest predicted 5PTases found in any organism. We report here that the At5PTase11 gene encodes an active 5PTase enzyme that can only dephosphorylate phosphoinositide substrates containing a 5-phosphate. In addition to hydrolyzing known substrates of 5PTase enzymes, At5PTase11 also hydrolyzes the 5-phosphate from phosphatidylinositol (3,5) bisphosphate. We also show that the At5PTase11 gene is regulated by abscisic acid, jasmonic acid, and auxin, suggesting a role for phosphoinositide action in these signal transduction pathways.

^[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.040253.

^* Corresponding author; e-mail gillaspy{at}vt.edu; fax 540–231–7126.

Received February 19, 2004; returned for revision April 4, 2004; accepted April 5, 2004.

This article has been cited by other articles:

				M. Regente, G. C. Monzon, and L. de la Canal Phospholipids are present in extracellular fluids of imbibing sunflower seeds and are modulated by hormonal treatments J. Exp. Bot., February 1, 2008; 59(3): 553 - 562. [Abstract] [Full Text] [PDF]

				Y. J. Im, I. Y. Perera, I. Brglez, A. J. Davis, J. Stevenson-Paulik, B. Q. Phillippy, E. Johannes, N. S. Allen, and W. F. Boss Increasing Plasma Membrane Phosphatidylinositol(4,5)Bisphosphate Biosynthesis Increases Phosphoinositide Metabolism in Nicotiana tabacum PLANT CELL, May 1, 2007; 19(5): 1603 - 1616. [Abstract] [Full Text] [PDF]

				B. Gunesekera, J. Torabinejad, J. Robinson, and G. E. Gillaspy Inositol Polyphosphate 5-Phosphatases 1 and 2 Are Required for Regulating Seedling Growth Plant Physiology, March 1, 2007; 143(3): 1408 - 1417. [Abstract] [Full Text] [PDF]

				W.-H. Lin, Y. Wang, B. Mueller-Roeber, C. A. Brearley, Z.-H. Xu, and H.-W. Xue At5PTase13 Modulates Cotyledon Vein Development through Regulating Auxin Homeostasis Plant Physiology, December 1, 2005; 139(4): 1677 - 1691. [Abstract] [Full Text] [PDF]

				M. E. Williams, J. Torabinejad, E. Cohick, K. Parker, E. J. Drake, J. E. Thompson, M. Hortter, and D. B. DeWald Mutations in the Arabidopsis Phosphoinositide Phosphatase Gene SAC9 Lead to Overaccumulation of PtdIns(4,5)P2 and Constitutive Expression of the Stress-Response Pathway Plant Physiology, June 1, 2005; 138(2): 686 - 700. [Abstract] [Full Text] [PDF]

				R. Zhong, D. H. Burk, C. J. Nairn, A. Wood-Jones, W. H. Morrison III, and Z.-H. Ye Mutation of SAC1, an Arabidopsis SAC Domain Phosphoinositide Phosphatase, Causes Alterations in Cell Morphogenesis, Cell Wall Synthesis, and Actin Organization PLANT CELL, May 1, 2005; 17(5): 1449 - 1466. [Abstract] [Full Text] [PDF]

				R. Zhong, D. H. Burk, W. H. Morrison III, and Z.-H. Ye FRAGILE FIBER3, an Arabidopsis Gene Encoding a Type II Inositol Polyphosphate 5-Phosphatase, Is Required for Secondary Wall Synthesis and Actin Organization in Fiber Cells PLANT CELL, December 1, 2004; 16(12): 3242 - 3259. [Abstract] [Full Text] [PDF]