First published online June 4, 2004; 10.1104/pp.104.039743
Plant Physiology 135:969-977 (2004)
© 2004 American Society of Plant Biologists
DEVELOPMENT AND HORMONE ACTION
Arabidopsis Trehalose-6-Phosphate Synthase 1 Is Essential for Normal Vegetative Growth and Transition to Flowering1
Anja J.H. van Dijken*,
Henriette Schluepmann and
Sjef C.M. Smeekens
Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands
In resurrection plants and yeast, trehalose has a function in stress protection, but the absence of measurable amounts of trehalose in other plants precludes such a function. The identification of a trehalose biosynthetic pathway in angiosperms raises questions on the function of trehalose metabolism in nonresurrection plants. We previously identified a mutant in the Arabidopsis trehalose biosynthesis gene AtTPS1. Plants homozygous for the tps1 mutation do not develop mature seeds (Eastmond et al., 2002). AtTPS1 expression analysis and the spatial and temporal activity of its promoter suggest that this gene is active outside the seed-filling stage of development as well. A generally low expression is observed in all organs analyzed, peaking in metabolic sinks such as flower buds, ripening siliques, and young rosette leaves. The arrested tps1/tps1 embryonic state could be rescued using a dexamethasone-inducible AtTPS1 expression system enabling generation of homozygous mutant plants. When depleted in AtTPS1 expression, such mutant plants show reduced root growth, which is correlated with a reduced root meristematic region. Moreover, tps1/tps1 plants are retarded in growth and remain generative during their lifetime. Absence of Trehalose-6-Phosphate Synthase 1 in Arabidopsis plants precludes transition to flowering.
1 This work was supported by the Dutch Science Foundation, CW-STW (grant no. 349–4657).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.039743.
* Corresponding author; e-mail a.j.h.vandijken{at}bio.uu.nl; fax 31–30–251–3655.
Received January 28, 2004;
returned for revision March 1, 2004;
accepted March 1, 2004.
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