Plant Physiology 135:1336-1345 (2004)
© 2004 American Society of Plant Biologists
BIOENERGETICS AND PHOTOSYNTHESIS
Expression in Multigene Families. Analysis of Chloroplast and Mitochondrial Proteases1
Galit Sinvany-Villalobo,
Olga Davydov,
Giora Ben-Ari,
Adi Zaltsman,
Alexander Raskind and
Zach Adam*
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel (G.S., G.B.-A., A.Z., A.R., Z.A.); and Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (O.D.)
The proteolytic machinery of chloroplasts and mitochondria in Arabidopsis consists primarily of three families of ATP-dependent proteases, Clp, Lon, and FtsH, and one family of ATP-independent proteases, DegP. However, the functional significance of the multiplicity of their genes is not clear. To test whether expression of specific isomers could be differently affected by growth conditions, we analyzed transcript abundance following short-term exposure to different environmental stimuli, using 70-mer oligonucleotide arrays. This analysis revealed variability in the response to high light and different temperatures within members of each family. Thirty out of the 41 tested genes were up-regulated in response to high light, including both chloroplast and mitochondrial isozymes, whereas only six and five genes responded to either high or low temperature, respectively. The extent of response was variable, ranging from 2- to 20-fold increase in the steady-state levels. Absolute transcript levels of the tested genes, compiled from one-channel arrays, were also variable. In general, transcripts encoding mitochondrial isozymes were accumulated to a lower level than chloroplastic ones. Within the FtsH family, transcript abundance of most genes correlated with the severity of mutant phenotypes in the relevant genes. This correlation was also evident at the protein level. Analysis of FtsH isozymes revealed that FtsH2 was the most abundant species, followed by FtsH5 and 8, with FtsH1 being accumulated to only 10% of FtsH2 level. These results suggest that, unlike previous expectations, the relative importance of different chloroplast protease isozymes, evidenced by mutant phenotypes at least in the FtsH family, is determined by their abundance, and not necessarily by different specific functions or specialized expression under certain conditions.
1 This work was supported in part by grants from the Israel Science Foundation and the U.S.-Israel Binational Agricultural Research and Development Fund (to Z.A.).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.043299.
* Corresponding author; e-mail zach{at}agri.huji.ac.il; fax 972–8–948–9329.
Received March 24, 2004;
returned for revision May 4, 2004;
accepted May 11, 2004.
This article has been cited by other articles:
|
|
|
|
 
W. G. van Doorn and E. J. Woltering
Physiology and molecular biology of petal senescence
J. Exp. Bot.,
March 3, 2008;
(2008)
erm356v2.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Ostersetzer, Y. Kato, Z. Adam, and W. Sakamoto
Multiple Intracellular Locations of Lon Protease in Arabidopsis: Evidence for the Localization of AtLon4 to Chloroplasts
Plant Cell Physiol.,
June 1, 2007;
48(6):
881 - 885.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Kapri-Pardes, L. Naveh, and Z. Adam
The Thylakoid Lumen Protease Deg1 Is Involved in the Repair of Photosystem II from Photoinhibition in Arabidopsis
PLANT CELL,
March 1, 2007;
19(3):
1039 - 1047.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. FERREIRA, K. HJERNO, M. LARSEN, G. WINGSLE, P. LARSEN, S. FEY, P. ROEPSTORFF, and M. SALOME PAIS
Proteome Profiling of Populus euphratica Oliv. Upon Heat Stress
Ann. Bot.,
August 1, 2006;
98(2):
361 - 377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Aluru, F. Yu, A. Fu, and S. Rodermel
Arabidopsis variegation mutants: new insights into chloroplast biogenesis
J. Exp. Bot.,
June 1, 2006;
57(9):
1871 - 1881.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Giacomelli, A. Rudella, and K. J. van Wijk
High Light Response of the Thylakoid Proteome in Arabidopsis Wild Type and the Ascorbate-Deficient Mutant vtc2-2. A Comparative Proteomics Study
Plant Physiology,
June 1, 2006;
141(2):
685 - 701.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Zaltsman, N. Ori, and Z. Adam
Two Types of FtsH Protease Subunits Are Required for Chloroplast Biogenesis and Photosystem II Repair in Arabidopsis
PLANT CELL,
October 1, 2005;
17(10):
2782 - 2790.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Zelisko, M. Garcia-Lorenzo, G. Jackowski, S. Jansson, and C. Funk
AtFtsH6 is involved in the degradation of the light-harvesting complex II during high-light acclimation and senescence
PNAS,
September 20, 2005;
102(38):
13699 - 13704.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Yu, S. Park, and S. R. Rodermel
Functional Redundancy of AtFtsH Metalloproteases in Thylakoid Membrane Complexes
Plant Physiology,
August 1, 2005;
138(4):
1957 - 1966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
