First published online June 25, 2004; 10.1104/pp.103.036384
Plant Physiology 135:1447-1456 (2004)
© 2004 American Society of Plant Biologists
CELL BIOLOGY AND SIGNAL TRANSDUCTION
Down-Regulation of Metallothionein, a Reactive Oxygen Scavenger, by the Small GTPase OsRac1 in Rice
Hann Ling Wong,
Tsuyoshi Sakamoto,
Tsutomu Kawasaki,
Kenji Umemura and
Ko Shimamoto*
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Takayama 8916–5, Ikoma, Nara 630–0101, Japan (H.L.W., T.S., T.K., K.S.); and Bioscience Laboratories, Meiji Seika Kaisha Ltd., 5–3–1, Chiyoda, Sakado-shi, Saitama 350–0289, Japan (K.U.)
Metallothioneins are small, ubiquitous Cys-rich proteins known to be involved in reactive oxygen species (ROS) scavenging and metal homeostasis. We found that the expression of a metallothionein gene (OsMT2b) was synergically down-regulated by OsRac1 and rice (Oryza sativa) blast-derived elicitors. Transgenic plants overexpressing OsMT2b showed increased susceptibility to bacterial blight and blast fungus. OsMT2b-overexpressing cells showed reduced elicitor-induced hydrogen peroxide production. In contrast, homozygous OsMT2b::Tos17-inserted mutant and OsMT2b-RNAi-silenced transgenic cells showed significantly higher elicitor-induced hydrogen peroxide production than the wild-type cells. In vitro assay showed that recombinant OsMT2b protein possessed superoxide- and hydroxyl radical-scavenging activities. Taken together, these results showed that OsMT2b is an ROS scavenger and its expression is down-regulated by OsRac1, thus potentiating ROS, which function as signals in resistance response. The results suggest that OsRac1 plays a dual role as an inducer of ROS production and a suppressor of ROS scavenging.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.036384.
* Corresponding author; e-mail: simamoto{at}bs.naist.jp; fax 81–743–72–5509.
Received November 19, 2003;
returned for revision January 29, 2004;
accepted February 1, 2004.
This article has been cited by other articles:
|
|
|
|
 
B. Steffens and M. Sauter
Heterotrimeric G Protein Signaling Is Required for Epidermal Cell Death in Rice
Plant Physiology,
October 1, 2009;
151(2):
732 - 740.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fujiwara, S. Hamada, M. Hiratsuka, Y. Fukao, T. Kawasaki, and K. Shimamoto
Proteome Analysis of Detergent-Resistant Membranes (DRMs) Associated with OsRac1-Mediated Innate Immunity in Rice
Plant Cell Physiol.,
July 1, 2009;
50(7):
1191 - 1200.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhang, C. Lian, and Z. Shen
Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa
Ann. Bot.,
April 1, 2009;
103(6):
923 - 930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Xue, X. Li, W. Zhu, C. Wu, G. Yang, and C. Zheng
Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast
J. Exp. Bot.,
January 1, 2009;
60(1):
339 - 349.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Steffens and M. Sauter
Epidermal Cell Death in Rice Is Confined to Cells with a Distinct Molecular Identity and Is Mediated by Ethylene and H2O2 through an Autoamplified Signal Pathway
PLANT CELL,
January 1, 2009;
21(1):
184 - 196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Yuksel and A. R. Memon
Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules
J. Exp. Bot.,
October 9, 2008;
(2008)
ern223v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nakashima, L. Chen, N. P. Thao, M. Fujiwara, H. L. Wong, M. Kuwano, K. Umemura, K. Shirasu, T. Kawasaki, and K. Shimamoto
RACK1 Functions in Rice Innate Immunity by Interacting with the Rac1 Immune Complex
PLANT CELL,
August 1, 2008;
20(8):
2265 - 2279.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Yuan, D. Chen, Y. Ren, X. Zhang, and J. Zhao
Characteristic and Expression Analysis of a Metallothionein Gene, OsMT2b, Down-Regulated by Cytokinin Suggests Functions in Root Development and Seed Embryo Germination of Rice
Plant Physiology,
April 1, 2008;
146(4):
1637 - 1650.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-J. Guo, M. Meetam, and P. B. Goldsbrough
Examining the Specific Contributions of Individual Arabidopsis Metallothioneins to Copper Distribution and Metal Tolerance
Plant Physiology,
April 1, 2008;
146(4):
1697 - 1706.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Abe, M. Fujiwara, K.-i. Kurotani, S. Yokoi, and K. Shimamoto
Identification of Dynamin as an Interactor of Rice GIGANTEA by Tandem Affinity Purification (TAP)
Plant Cell Physiol.,
March 1, 2008;
49(3):
420 - 432.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Singh, P. J. Kang, and H.-O. Park
The Rho5 GTPase is necessary for oxidant-induced cell death in budding yeast
PNAS,
February 5, 2008;
105(5):
1522 - 1527.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. P. Thao, L. Chen, A. Nakashima, S.-i. Hara, K. Umemura, A. Takahashi, K. Shirasu, T. Kawasaki, and K. Shimamoto
RAR1 and HSP90 Form a Complex with Rac/Rop GTPase and Function in Innate-Immune Responses in Rice
PLANT CELL,
December 1, 2007;
19(12):
4035 - 4045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. L. Wong, R. Pinontoan, K. Hayashi, R. Tabata, T. Yaeno, K. Hasegawa, C. Kojima, H. Yoshioka, K. Iba, T. Kawasaki, et al.
Regulation of Rice NADPH Oxidase by Binding of Rac GTPase to Its N-Terminal Extension
PLANT CELL,
December 1, 2007;
19(12):
4022 - 4034.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Meyer, R. A. Damveld, M. Arentshorst, U. Stahl, C. A. M. J. J. van den Hondel, and A. F. J. Ram
Survival in the Presence of Antifungals: GENOME-WIDE EXPRESSION PROFILING OF ASPERGILLUS NIGER IN RESPONSE TO SUBLETHAL CONCENTRATIONS OF CASPOFUNGIN AND FENPROPIMORPH
J. Biol. Chem.,
November 9, 2007;
282(45):
32935 - 32948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-C. Leple, R. Dauwe, K. Morreel, V. Storme, C. Lapierre, B. Pollet, A. Naumann, K.-Y. Kang, H. Kim, K. Ruel, et al.
Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure
PLANT CELL,
November 1, 2007;
19(11):
3669 - 3691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Heise, S. Krejci, J. Miersch, G.-J. Krauss, and K. Humbeck
Gene Expression of Metallothioneins in Barley during Senescence and Heavy Metal Treatment
Crop Sci.,
May 31, 2007;
47(3):
1111 - 1118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fujiwara, K. Umemura, T. Kawasaki, and K. Shimamoto
Proteomics of Rac GTPase Signaling Reveals Its Predominant Role in Elicitor-Induced Defense Response of Cultured Rice Cells
Plant Physiology,
February 1, 2006;
140(2):
734 - 745.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kawasaki, H. Koita, T. Nakatsubo, K. Hasegawa, K. Wakabayashi, H. Takahashi, K. Umemura, T. Umezawa, and K. Shimamoto
Cinnamoyl-CoA reductase, a key enzyme in lignin biosynthesis, is an effector of small GTPase Rac in defense signaling in rice
PNAS,
January 3, 2006;
103(1):
230 - 235.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Lieberherr, N. P. Thao, A. Nakashima, K. Umemura, T. Kawasaki, and K. Shimamoto
A Sphingolipid Elicitor-Inducible Mitogen-Activated Protein Kinase Is Regulated by the Small GTPase OsRac1 and Heterotrimeric G-Protein in Rice
Plant Physiology,
July 1, 2005;
138(3):
1644 - 1652.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
