First published online July 2, 2004; 10.1104/pp.104.041400
Plant Physiology 135:1595-1607 (2004)
© 2004 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
Expression Profiling-Based Identification of CO2-Responsive Genes Regulated by CCM1 Controlling a Carbon-Concentrating Mechanism in Chlamydomonas reinhardtii1
Kenji Miura,
Takashi Yamano,
Satoshi Yoshioka,
Tsutomu Kohinata,
Yoshihiro Inoue,
Fumiya Taniguchi,
Erika Asamizu,
Yasukazu Nakamura,
Satoshi Tabata,
Katsuyuki T. Yamato,
Kanji Ohyama and
Hideya Fukuzawa*
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, 606–8502, Japan (K.M., T.Y., S.Y., T.K., Y.I., F.T., K.T.Y., K.O., H.F.); and Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (E.A., Y.N., S.T.)
Photosynthetic acclimation to CO2-limiting stress is associated with control of genetic and physiological responses through a signal transduction pathway, followed by integrated monitoring of the environmental changes. Although several CO2-responsive genes have been previously isolated, genome-wide analysis has not been applied to the isolation of CO2-responsive genes that may function as part of a carbon-concentrating mechanism (CCM) in photosynthetic eukaryotes. By comparing expression profiles of cells grown under CO2-rich conditions with those of cells grown under CO2-limiting conditions using a cDNA membrane array containing 10,368 expressed sequence tags, 51 low-CO2 inducible genes and 32 genes repressed by low CO2 whose mRNA levels were changed more than 2.5-fold in Chlamydomonas reinhardtii Dangeard were detected. The fact that the induction of almost all low-CO2 inducible genes was impaired in the ccm1 mutant suggests that CCM1 is a master regulator of CCM through putative low-CO2 signal transduction pathways. Among low-CO2 inducible genes, two novel genes, LciA and LciB, were identified, which may be involved in inorganic carbon transport. Possible functions of low-CO2 inducible and/or CCM1-regulated genes are discussed in relation to the CCM.
1 This work was supported by the Japanese Ministry of Education, Science and Culture (grant nos. 14656136 and 15380071), by the Japan Society for the Promotion of Science (grant no. JSPS–RFTF97R16001 to H.F.), and by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (grant no. 3117 to K.M.).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.041400.
* Corresponding author; e-mail fukuzawa{at}lif.kyoto-u.ac.jp; fax 81–75–753–6127.
Received February 21, 2004;
returned for revision March 27, 2004;
accepted April 18, 2004.
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