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ENVIRONMENTAL STRESS AND ADAPTATION

-Amylase Induction and the Protective Role of Maltose during Temperature Shock¹

Plant Molecular and Cellular Biology Program, Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32611–0675

A number of studies have demonstrated -amylase induction in response to abiotic stress. In the present work, a temperature response profile in 5°C increments from 45°C to 0°C showed that induction at temperature extremes was specific for two members of the gene family (BMY7 and BMY8). Both members encode proteins that possess apparent transit peptides for chloroplast stromal localization. However, induction was not observed for other key starch degrading enzymes demonstrating a rather specific response to temperature stress for BMY7 and BMY8. Time course experiments for heat shock at 40°C and cold shock at 5°C showed that -amylase induction correlated with maltose accumulation. Maltose has the ability, as demonstrated by in vitro assays, to protect proteins, membranes, and the photosynthetic electron transport chain at physiologically relevant concentrations. Therefore, -amylase induction and the resultant maltose accumulation may function as a compatible-solute stabilizing factor in the chloroplast stroma in response to acute temperature stress.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.040808.

^* Corresponding author; e-mail clguy{at}ufl.edu; fax 352–392–1413.

Received February 11, 2004; returned for revision March 25, 2004; accepted March 25, 2004.

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