First published online July 9, 2004; 10.1104/pp.104.039909
Plant Physiology 135:1697-1709 (2004)
© 2004 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
Comparative Genomics in Salt Tolerance between Arabidopsis and Arabidopsis-Related Halophyte Salt Cress Using Arabidopsis Microarray1
Teruaki Taji,
Motoaki Seki,
Masakazu Satou,
Tetsuya Sakurai,
Masatomo Kobayashi,
Kanako Ishiyama,
Yoshihiro Narusaka,
Mari Narusaka,
Jian-Kang Zhu and
Kazuo Shinozaki*
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305–0074, Japan (T.T., M.S., Y.N., K.S.); Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Tsurumi-ku, Yokohama 230–0045, Japan (M.S., M.S., T.S., M.N.); Genomic Knowledge Base Research Team, Bioinformatics Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Tsurumi-ku, Yokohama 230–0045, Japan (M.S., T.S.); Experimental Plant Division, RIKEN BioResource Center, Tsukuba, Ibaraki 305–0074, Japan (M.K., K.I.); Department of Biology, Tokyo Gakugei University, Koganei-shi, Tokyo 184–8501, Japan (Y.N., M.N.); and Institute of Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521 (J.K.Z.)
Salt cress (Thellungiella halophila), a halophyte, is a genetic model system with a small plant size, short life cycle, copious seed production, small genome size, and an efficient transformation. Its genes have a high sequence identity (90%–95% at cDNA level) to genes of its close relative, Arabidopsis. These qualities are advantageous not only in genetics but also in genomics, such as gene expression profiling using Arabidopsis cDNA microarrays. Although salt cress plants are salt tolerant and can grow in 500 mM NaCl medium, they do not have salt glands or other morphological alterations either before or after salt adaptation. This suggests that the salt tolerance in salt cress results from mechanisms that are similar to those operating in glycophytes. To elucidate the differences in the regulation of salt tolerance between salt cress and Arabidopsis, we analyzed the gene expression profiles in salt cress by using a full-length Arabidopsis cDNA microarray. In salt cress, only a few genes were induced by 250 mM NaCl stress in contrast to Arabidopsis. Notably a large number of known abiotic- and biotic-stress inducible genes, including Fe-SOD, P5CS, PDF1.2, AtNCED, P-protein,  -glucosidase, and SOS1, were expressed in salt cress at high levels even in the absence of stress. Under normal growing conditions, salt cress accumulated Pro at much higher levels than did Arabidopsis, and this corresponded to a higher expression of AtP5CS in salt cress, a key enzyme of Pro biosynthesis. Furthermore, salt cress was more tolerant to oxidative stress than Arabidopsis. Stress tolerance of salt cress may be due to constitutive overexpression of many genes that function in stress tolerance and that are stress inducible in Arabidopsis.
1 This work was supported in part by the Genome Research Project in RIKEN (to K.S.) and by the Special Postdoctoral Researchers' Program from RIKEN (to T.T.).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.039909.
* Corresponding author; e-mail sinozaki{at}rtc.riken.go.jp; fax 81–29–836–4359.
Received January 29, 2004;
returned for revision April 7, 2004;
accepted April 8, 2004.
Related articles in Plant Physiol.:
-
- Peter V. Minorsky
Plant Physiol. 2004 135: 1147-1148.
[Full Text]
This article has been cited by other articles:
|
|
|
|
 
Y. O. Ahn, B.-i. Shimizu, K. Sakata, D. Gantulga, Z. Zhou, D. R. Bevan, and A. Esen
Scopolin-hydrolyzing {beta}-glucosidases in roots of Arabidopsis
Plant Cell Physiol.,
January 1, 2010;
51(1):
132 - 143.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-B. Luo, D. Janz, X. Jiang, C. Gobel, H. Wildhagen, Y. Tan, H. Rennenberg, I. Feussner, and A. Polle
Upgrading Root Physiology for Stress Tolerance by Ectomycorrhizas: Insights from Metabolite and Transcriptional Profiling into Reprogramming for Stress Anticipation
Plant Physiology,
December 1, 2009;
151(4):
1902 - 1917.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Edelist, X. Raffoux, M. Falque, C. Dillmann, D. Sicard, L. H. Rieseberg, and S. Karrenberg
Differential expression of candidate salt-tolerance genes in the halophyte Helianthus paradoxus and its glycophyte progenitors H. annuus and H. petiolaris (Asteraceae)
Am. J. Botany,
October 1, 2009;
96(10):
1830 - 1838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D.-H. Oh, E. Leidi, Q. Zhang, S.-M. Hwang, Y. Li, F. J. Quintero, X. Jiang, M. P. D'Urzo, S. Y. Lee, Y. Zhao, et al.
Loss of Halophytism by Interference with SOS1 Expression
Plant Physiology,
September 1, 2009;
151(1):
210 - 222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Tada and T. Kashimura
Proteomic Analysis of Salt-Responsive Proteins in the Mangrove Plant, Bruguiera gymnorhiza
Plant Cell Physiol.,
March 1, 2009;
50(3):
439 - 446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Stepien and G. N. Johnson
Contrasting Responses of Photosynthesis to Salt Stress in the Glycophyte Arabidopsis and the Halophyte Thellungiella: Role of the Plastid Terminal Oxidase as an Alternative Electron Sink
Plant Physiology,
February 1, 2009;
149(2):
1154 - 1165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Amtmann
Learning from Evolution: Thellungiella Generates New Knowledge on Essential and Critical Components of Abiotic Stress Tolerance in Plants
Mol Plant,
January 1, 2009;
2(1):
3 - 12.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Y. Perera, C.-Y. Hung, C. D. Moore, J. Stevenson-Paulik, and W. F. Boss
Transgenic Arabidopsis Plants Expressing the Type 1 Inositol 5-Phosphatase Exhibit Increased Drought Tolerance and Altered Abscisic Acid Signaling
PLANT CELL,
October 1, 2008;
20(10):
2876 - 2893.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kant, Y.-M. Bi, E. Weretilnyk, S. Barak, and S. J. Rothstein
The Arabidopsis Halophytic Relative Thellungiella halophila Tolerates Nitrogen-Limiting Conditions by Maintaining Growth, Nitrogen Uptake, and Assimilation
Plant Physiology,
July 1, 2008;
147(3):
1168 - 1180.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. de Lorenzo, F. Merchan, S. Blanchet, M. Megias, F. Frugier, M. Crespi, and C. Sousa
Differential Expression of the TFIIIA Regulatory Pathway in Response to Salt Stress between Medicago truncatula Genotypes
Plant Physiology,
December 1, 2007;
145(4):
1521 - 1532.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-M. Wang, J.-L. Zhang, and T. J. Flowers
Low-Affinity Na+ Uptake in the Halophyte Suaeda maritima
Plant Physiology,
October 1, 2007;
145(2):
559 - 571.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. N. Talke, M. Hanikenne, and U. Kramer
Zinc-Dependent Global Transcriptional Control, Transcriptional Deregulation, and Higher Gene Copy Number for Genes in Metal Homeostasis of the Hyperaccumulator Arabidopsis halleri
Plant Physiology,
September 1, 2006;
142(1):
148 - 167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Wong, Y. Li, A. Labbe, D. Guevara, P. Nuin, B. Whitty, C. Diaz, G. B. Golding, G. R. Gray, E. A. Weretilnyk, et al.
Transcriptional Profiling Implicates Novel Interactions between Abiotic Stress and Hormonal Responses in Thellungiella, a Close Relative of Arabidopsis
Plant Physiology,
April 1, 2006;
140(4):
1437 - 1450.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ma, Q. Gong, and H. J. Bohnert
Dissecting salt stress pathways
J. Exp. Bot.,
March 1, 2006;
57(5):
1097 - 1107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Wang, R. J. Davenport, V. Volkov, and A. Amtmann
Low unidirectional sodium influx into root cells restricts net sodium accumulation in Thellungiella halophila, a salt-tolerant relative of Arabidopsis thaliana
J. Exp. Bot.,
March 1, 2006;
57(5):
1161 - 1170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cuartero, M. C. Bolarin, M. J. Asins, and V. Moreno
Increasing salt tolerance in the tomato
J. Exp. Bot.,
March 1, 2006;
57(5):
1045 - 1058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Ottow, M. Brinker, T. Teichmann, E. Fritz, W. Kaiser, M. Brosche, J. Kangasjarvi, X. Jiang, and A. Polle
Populus euphratica Displays Apoplastic Sodium Accumulation, Osmotic Adjustment by Decreases in Calcium and Soluble Carbohydrates, and Develops Leaf Succulence under Salt Stress
Plant Physiology,
December 1, 2005;
139(4):
1762 - 1772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Vanneste, B. De Rybel, G. T.S. Beemster, K. Ljung, I. De Smet, G. Van Isterdael, M. Naudts, R. Iida, W. Gruissem, M. Tasaka, et al.
Cell Cycle Progression in the Pericycle Is Not Sufficient for SOLITARY ROOT/IAA14-Mediated Lateral Root Initiation in Arabidopsis thaliana
PLANT CELL,
November 1, 2005;
17(11):
3035 - 3050.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Vera-Estrella, B. J. Barkla, L. Garcia-Ramirez, and O. Pantoja
Salt Stress in Thellungiella halophila Activates Na+ Transport Mechanisms Required for Salinity Tolerance
Plant Physiology,
November 1, 2005;
139(3):
1507 - 1517.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Amtmann, H. J. Bohnert, and R. A. Bressan
Abiotic Stress and Plant Genome Evolution. Search for New Models
Plant Physiology,
May 1, 2005;
138(1):
127 - 130.
[Full Text]
[PDF]
|
|
|
|
|
