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First published online August 13, 2004; 10.1104/pp.104.041442

Plant Physiology 135:2150-2161 (2004)
© 2004 American Society of Plant Biologists

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CELL BIOLOGY AND SIGNAL TRANSDUCTION

Pathogen- and NaCl-Induced Expression of the SCaM-4 Promoter Is Mediated in Part by a GT-1 Box That Interacts with a GT-1-Like Transcription Factor1

Hyeong Cheol Park2, Man Lyang Kim, Yun Hwan Kang, Joo Mi Jeon, Jae Hyuk Yoo, Min Chul Kim, Chan Young Park, Jae Cheol Jeong, Byeong Cheol Moon, Ju Huck Lee, Hae Won Yoon, Sung-Ho Lee, Woo Sik Chung, Chae Oh Lim, Sang Yeol Lee, Jong Chan Hong and Moo Je Cho*

Division of Applied Life Science (BK21 Program), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660–701, Korea

The Ca2+-binding protein calmodulin mediates cellular Ca2+ signals in response to a wide array of stimuli in higher eukaryotes. Plants express numerous CaM isoforms. Transcription of one soybean (Glycine max) CaM isoform, SCaM-4, is dramatically induced within 30 min of pathogen or NaCl stresses. To characterize the cis-acting element(s) of this gene, we isolated an approximately 2-kb promoter sequence of the gene. Deletion analysis of the promoter revealed that a 130-bp region located between nucleotide positions –858 and –728 is required for the stressors to induce expression of SCaM-4. A hexameric DNA sequence within this region, GAAAAA (GT-1 cis-element), was identified as a core cis-acting element for the induction of the SCaM-4 gene. The GT-1 cis-element interacts with an Arabidopsis GT-1-like transcription factor, AtGT-3b, in vitro and in a yeast selection system. Transcription of AtGT-3b is also rapidly induced within 30 min after pathogen and NaCl treatment. These results suggest that an interaction between a GT-1 cis-element and a GT-1-like transcription factor plays a role in pathogen- and salt-induced SCaM-4 gene expression in both soybean and Arabidopsis.

1 This work was supported by a Basic Research Grant (grant no. R02–2002–000–00009–0), by the National Research Laboratory program (2000–N–NL–01–C–236), by the Crop Functional Genomics Center of the 21st Century Frontier Research Program CG1512, by the Gyeongnam High Tech Foundation (2001), and by the Ministry of Agriculture and Forestry (grant no. 298049–4 to M.J.C.), and partially by the Environmental Biotechnology Research Center (grant no. R15–2003–012–02003–0), by the Crop Functional Genomics Center of the 21st Century Frontier Research Program CG1124, and by the Center for Plant Molecular and Genetic Breeding Research, KOSEF in Korea (grant to J.C.H.). Y.H.K., C.Y.P., and B.C.M. were supported by scholarships from the BK21 program, Ministry of Education and Human Resources Development in Korea.

2 Present address: Department of Biology, Coker Hall, Room 108, CB 3280, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.041442.

* Corresponding authors; e-mail choslab{at}nongae.gsnu.ac.kr; fax 82–55–759–9363.

Received February 22, 2004; returned for revision May 20, 2004; accepted May 24, 2004.




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