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CELL BIOLOGY AND SIGNAL TRANSDUCTION

Characterization of the Arabidopsis Lysine-Rich Arabinogalactan-Protein AtAGP17 Mutant (rat1) That Results in a Decreased Efficiency of Agrobacterium Transformation^1,[w]

Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Victoria 3010, Australia (Y.M.G., P.R.G., A.B.); Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907–1392 (J.N., L.-Y.L., S.B.G.); and School of Agriculture and Wine, Waite Agricultural Research Institute, The University of Adelaide, South Australia 5064, Australia (C.J.S.)

Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. The Arabidopsis rat1 mutant, previously characterized as resistant to Agrobacterium tumefaciens root transformation, is due to a mutation in the gene for the Lys-rich AGP, AtAGP17. We show that the phenotype of rat1 correlates with down-regulation of AGP17 in the root as a result of a T-DNA insertion into the promoter of AGP17. Complementation of rat1 plants by a floral dip method with either the wild-type AGP17 gene or cDNA can restore the plant to a wild-type phenotype in several independent transformants. Based on changes in PR1 gene expression and a decrease in free salicylic acid levels upon Agrobacterium infection, we suggest mechanisms by which AGP17 allows Agrobacterium rapidly to reduce the systemic acquired resistance response during the infection process.

² Present address: Faculty of Natural Resources and Life Sciences, Dong-A University, Pusan 604–714, South Korea.

³ Present address: The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia.

^[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.045542.

^* Corresponding author; e-mail abacic{at}unimelb.edu.au; fax 61–3–9347–1071.

Received April 29, 2004; returned for revision June 1, 2004; accepted June 2, 2004.

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