First published online October 1, 2004; 10.1104/pp.104.047126
Plant Physiology 136:2961-2970 (2004)
© 2004 American Society of Plant Biologists
Requirement of the Histidine Kinase Domain for Signal Transduction by the Ethylene Receptor ETR11
Xiang Qu and
G. Eric Schaller*
Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, New Hampshire 03824 (X.Q.); and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755 (G.E.S.)
In Arabidopsis, ethylene is perceived by a receptor family consisting of five members, one of these being ETR1. The N-terminal half of ETR1 functions as a signal input domain. The C-terminal region of ETR1, consisting of a His kinase domain and a putative receiver domain, is likely to function in signal output. The role of the proposed signal output region in ethylene signaling was examined in planta. For this purpose, the ability of mutant versions of ETR1 to rescue the constitutive ethylene-response phenotype of the etr1-6;etr2-3;ein4-4 triple loss-of-function mutant line was examined. A truncated version of ETR1 that lacks both the His kinase domain and the receiver domain failed to rescue the triple mutant phenotype. A truncated ETR1 receptor that lacks only the receiver domain restored normal growth to the triple mutant in air, but the transgenic seedlings displayed hypersensitivity to low doses of ethylene. A mutation that eliminated His kinase activity had a modest effect upon the ability of the receptor to repress ethylene responses in air. These results demonstrate that the His kinase domain plays a role in the repression of ethylene responses. The potential roles of the receiver domain and His kinase activity in ethylene signaling are discussed.
1 This work was supported by the National Science Foundation (grant nos. MCB–9982510 and MCB–0235450 to G.E.S.) and by a University of New Hampshire graduate research fellowship (to X.Q).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.047126.
* Corresponding author; e-mail george.e.schaller{at}dartmouth.edu; fax 603–646–1347.
Received May 26, 2004;
returned for revision July 19, 2004;
accepted August 31, 2004.
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