This Article Full Text Full Text (PDF) All Versions of this Article: 136/3/3562    most recent pp.104.046961v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (25) Citing Articles via Google Scholar Google Scholar Articles by Iwano, M. Articles by Isogai, A. Search for Related Content PubMed PubMed Citation Articles by Iwano, M. Articles by Isogai, A. Agricola Articles by Iwano, M. Articles by Isogai, A.

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Ca²⁺ Dynamics in a Pollen Grain and Papilla Cell during Pollination of Arabidopsis¹

Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0101, Japan (M.I., H.S., T.M., F.-S.C., S.T., A.I.); and The Institute of Physical and Chemical Research, Wako, Saitama 351–0198, Japan (T.N., A.M.)

Ca²⁺ dynamics in the growing pollen tube have been well documented in vitro using germination assays and Ca²⁺ imaging techniques. However, very few in vivo studies of Ca²⁺ in the pollen grain and papilla cell during pollination have been performed. We expressed yellow cameleon, a Ca²⁺ indicator based on green fluorescent protein, in the pollen grains and papilla cells of Arabidopsis (Arabidopsis thaliana) and monitored Ca²⁺ dynamics during pollination. In the pollen grain, [Ca²⁺]_cyt increased at the potential germination site soon after hydration and remained augmented until germination. As in previous in vitro germination studies, [Ca²⁺]_cyt oscillations were observed in the tip region of the growing pollen tube, but the oscillation frequency was faster and [Ca²⁺]_cyt was higher than had been observed in vitro. In the pollinated papilla cell, remarkable increases in [Ca²⁺]_cyt occurred three times in succession, just under the site of pollen-grain attachment. [Ca²⁺]_cyt increased first soon after pollen hydration, with a second increase occurring after pollen protrusion. The third and most remarkable [Ca²⁺]_cyt increase took place when the pollen tube penetrated into the papilla cell wall.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.046961.

^* Corresponding author; e-mail m-iwano{at}bs.naist.jp; fax 81–743–72–5459.

Received May 25, 2004; returned for revision August 8, 2004; accepted August 9, 2004.

This article has been cited by other articles:

				M. Iwano, T. Entani, H. Shiba, M. Kakita, T. Nagai, H. Mizuno, A. Miyawaki, T. Shoji, K. Kubo, A. Isogai, et al. Fine-Tuning of the Cytoplasmic Ca2+ Concentration Is Essential for Pollen Tube Growth Plant Physiology, July 1, 2009; 150(3): 1322 - 1334. [Abstract] [Full Text] [PDF]

				T. Wang, Y. Xiang, J. Hou, and H.-Y. Ren ABP41 is Involved in the Pollen Tube Development via Fragmenting Actin Filaments Mol Plant, November 20, 2008; (2008) ssn073v1. [Abstract] [Full Text] [PDF]

				J. Sun, H. Miwa, J. A. Downie, and G. E.D. Oldroyd Mastoparan Activates Calcium Spiking Analogous to Nod Factor-Induced Responses in Medicago truncatula Root Hair Cells Plant Physiology, June 1, 2007; 144(2): 695 - 702. [Abstract] [Full Text] [PDF]

				M. Iwano, H. Shiba, K. Matoba, T. Miwa, M. Funato, T. Entani, P. Nakayama, H. Shimosato, A. Takaoka, A. Isogai, et al. Actin Dynamics in Papilla Cells of Brassica rapa during Self- and Cross-Pollination Plant Physiology, May 1, 2007; 144(1): 72 - 81. [Abstract] [Full Text] [PDF]

				M. Bosch and P. K. Hepler Pectin Methylesterases and Pectin Dynamics in Pollen Tubes PLANT CELL, December 1, 2005; 17(12): 3219 - 3226. [Full Text] [PDF]

				R. A. Cole, L. Synek, V. Zarsky, and J. E. Fowler SEC8, a Subunit of the Putative Arabidopsis Exocyst Complex, Facilitates Pollen Germination and Competitive Pollen Tube Growth Plant Physiology, August 1, 2005; 138(4): 2005 - 2018. [Abstract] [Full Text] [PDF]