First published online October 29, 2004; 10.1104/pp.104.049189
Plant Physiology 136:3639-3648 (2004)
© 2004 American Society of Plant Biologists
DEVELOPMENT AND HORMONE ACTION
ARIA, an Arabidopsis Arm Repeat Protein Interacting with a Transcriptional Regulator of Abscisic Acid-Responsive Gene Expression, Is a Novel Abscisic Acid Signaling Component1
Sunmi Kim,
Hyung-in Choi,
Hyun-Ju Ryu,
Ji Hye Park,
Myoung Duck Kim and
Soo Young Kim*
Kumho Life and Environmental Science Laboratory, Korea Kumho Petrochemical, Gwangju 500–712, South Korea
Arabidopsis (Arabidopsis thaliana) genome contains more than 90 armadillo (arm) repeat proteins. However, their functions are largely unknown. Here, we report that an Arabidopsis arm repeat protein is involved in abscisic acid (ABA) response. We carried out two-hybrid screens to identify signaling components that modulate ABA-responsive gene expression. Employing a transcription factor, ABF2, which controls the ABA-dependent gene expression via the G-box type ABA-responsive elements, we isolated an arm repeat protein. The ABF2-interacting protein, designated as ARIA (arm repeat protein interacting with ABF2), has another conserved sequence motif, BTB/POZ (broad complex, tramtrak, and bric-a-brac/poxvirus and zinc finger) domain, in the C-terminal region. The physiological relevance of ABF2-ARIA interaction was supported by their similar expression patterns and similar subcellular localization. Plants overexpressing ARIA are hypersensitive to ABA and high osmolarity during germination and insensitive to salt during subsequent seedling growth. By contrast, an ARIA knockout mutant exhibits ABA and glucose insensitivities. Changes in the expression levels of several ABF2-regulated genes were also observed in ARIA overexpression lines, indicating that ARIA modulates the transcriptional activity of ABF2. Together, our data indicate that ARIA is a positive regulator of ABA response.
1 This work was supported in part by grants from the Crop Functional Genomics Center of the 21C Frontier Program funded by the Ministry of Science and Technology of Korea and by the Agricultural Plant Stress Research Center of Chonnam National University funded by the Korea Science and Engineering Foundation (to S.Y.K.). This paper is Kumho Life and Environmental Science Laboratory publication number 73.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.049189.
* Corresponding author; e-mail sooykim{at}kkpc.com; fax 82–62–972–5085.
Received July 5, 2004;
returned for revision August 24, 2004;
accepted August 28, 2004.
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