First published online October 29, 2004; 10.1104/pp.104.051318
Plant Physiology 136:3724-3736 (2004)
© 2004 American Society of Plant Biologists
BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES
The Biochemical and Molecular Basis for the Divergent Patterns in the Biosynthesis of Terpenes and Phenylpropenes in the Peltate Glands of Three Cultivars of Basil1
Yoko Iijima,
Rachel Davidovich-Rikanati,
Eyal Fridman,
David R. Gang,
Einat Bar,
Efraim Lewinsohn and
Eran Pichersky*
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109–1048 (Y.I., E.F., E.P.); Department of Plant Sciences and Institute for Biomedical Science and Biotechnology, University of Arizona, Tucson, Arizona 85721–0036 (D.R.G.); and Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (R.D.-R., E.B., E.L.)
Surface glandular trichomes distributed throughout the aerial parts of sweet basil (Ocimum basilicum) produce and store monoterpene, sesquiterpene, and phenylpropene volatiles. Three distinct basil chemotypes were used to examine the molecular mechanisms underlying the divergence in their monoterpene and sesquiterpene content. The relative levels of specific terpenes in the glandular trichomes of each cultivar were correlated with the levels of transcripts for eight genes encoding distinct terpene synthases. In a cultivar that produces mostly (R)-linalool, transcripts of (R)-linalool synthase (LIS) were the most abundant of these eight. In a cultivar that synthesizes mostly geraniol, transcripts of geraniol synthase were the most abundant, but the glands of this cultivar also contained a transcript of an (R)-LIS gene with a 1-base insertion that caused a frameshift mutation. A geraniol synthase-LIS hybrid gene was constructed and expressed in Escherichia coli, and the protein catalyzed the formation of both geraniol and (R)-linalool from geranyl diphosphate. The total amounts of terpenes were correlated with total levels of terpene synthase activities, and negatively correlated with levels of phenylpropanoids and phenylalanine ammonia lyase activity. The relative levels of geranyl diphosphate synthase and farnesyl diphosphate synthase activities did not correlate with the total amount of terpenes produced, but showed some correlation with the ratio of monoterpenes to sesquiterpenes.
1 This work was supported by the U.S. Department of Agriculture-Binational Agricultural Research and Development Fund (grant no. IS–3332–02C to E.P. and E.L.), by the National Research Initiative Competitive Grants Program-U.S. Department of Agriculture (grant no. 2001–35318–10006 to E.P.), by the Vaadia-Binational Agricultural Research and Development Fund postdoctoral fellowship (FI–328–2002 to E.F.), and by the National Science Foundation (grant no. MCB–0210170 to D.R.G.).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.051318.
* Corresponding author; e-mail lelx{at}umich.edu; fax 734–647–0884.
Received August 6, 2004;
returned for revision September 2, 2004;
accepted September 3, 2004.
This article has been cited by other articles:
|
|
|
|
 
M. J. Davies, C. J. Atkinson, C. Burns, J. G. Woolley, N. A. Hipps, R. R. J. Arroo, N. Dungey, T. Robinson, P. Brown, I. Flockart, et al.
Enhancement of artemisinin concentration and yield in response to optimization of nitrogen and potassium supply to Artemisia annua
Ann. Bot.,
August 1, 2009;
104(2):
315 - 323.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Koeduka, T. J. Baiga, J. P. Noel, and E. Pichersky
Biosynthesis of t-Anethole in Anise: Characterization of t-Anol/Isoeugenol Synthase and an O-Methyltransferase Specific for a C7-C8 Propenyl Side Chain
Plant Physiology,
January 1, 2009;
149(1):
384 - 394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Besser, A. Harper, N. Welsby, I. Schauvinhold, S. Slocombe, Y. Li, R. A. Dixon, and P. Broun
Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species
Plant Physiology,
January 1, 2009;
149(1):
499 - 514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Jakoby, D. Falkenhan, M. T. Mader, G. Brininstool, E. Wischnitzki, N. Platz, A. Hudson, M. Hulskamp, J. Larkin, and A. Schnittger
Transcriptional Profiling of Mature Arabidopsis Trichomes Reveals That NOECK Encodes the MIXTA-Like Transcriptional Regulator MYB106
Plant Physiology,
November 1, 2008;
148(3):
1583 - 1602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. G. Kollner, C. Schnee, S. Li, A. Svatos, B. Schneider, J. Gershenzon, and J. Degenhardt
Protonation of a Neutral (S)-{beta}-Bisabolene Intermediate Is Involved in (S)-{beta}-Macrocarpene Formation by the Maize Sesquiterpene Synthases TPS6 and TPS11
J. Biol. Chem.,
July 25, 2008;
283(30):
20779 - 20788.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lee and J. Chappell
Biochemical and Genomic Characterization of Terpene Synthases in Magnolia grandiflora
Plant Physiology,
July 1, 2008;
147(3):
1017 - 1033.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Spitzer, M. M. B. Zvi, M. Ovadis, E. Marhevka, O. Barkai, O. Edelbaum, I. Marton, T. Masci, M. Alon, S. Morin, et al.
Reverse Genetics of Floral Scent: Application of Tobacco Rattle Virus-Based Gene Silencing in Petunia
Plant Physiology,
December 1, 2007;
145(4):
1241 - 1250.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kapteyn, A. V. Qualley, Z. Xie, E. Fridman, N. Dudareva, and D. R. Gang
Evolution of Cinnamate/p-Coumarate Carboxyl Methyltransferases and Their Role in the Biosynthesis of Methylcinnamate
PLANT CELL,
October 1, 2007;
19(10):
3212 - 3229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Fridman, J. Wang, Y. Iijima, J. E. Froehlich, D. R. Gang, J. Ohlrogge, and E. Pichersky
Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of Methylketones
PLANT CELL,
April 1, 2005;
17(4):
1252 - 1267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
