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CELL BIOLOGY AND SIGNAL TRANSDUCTION

Progression through Meiosis I and Meiosis II in Arabidopsis Anthers Is Regulated by an A-Type Cyclin Predominately Expressed in Prophase I¹

Department of Botany, Oklahoma State University, Stillwater, Oklahoma 74078 (Y.W., M.Y.); and Plant Gene Expression Center, United States Department of Agriculture, Agricultural Research Service, and Department of Plant and Microbial Biology, University of California at Berkeley, Albany, California 94710 (J.-L.M., S.M.)

Meiosis is often described as a special case of cell division since it differs from mitosis in having two nuclear divisions without an intervening S-phase. It will be of great interest to uncover what molecular mechanisms underlie these special features of meiosis. We previously reported that the tardy asynchronous meiosis (tam) mutant of Arabidopsis (Arabidopsis thaliana) is slower in cell cycle progression in male meiosis. Here we report that TAM encodes the A-type cyclin, CYCA1;2. The point mutation in tam replaced a conserved threonine with an isoleucine in the linker region between the 4 and 5 helices of the first cyclin fold. By studying the dynamics of a CYCA1;2-green fluorescent protein fusion protein under the control of the CYCA1;2 promoter, we found that the fusion protein was most abundant at pachytene, but was undetectable from late prophase I until telophase II. Nonetheless, cell cycle progression in tam was delayed in both pachytene and meiosis II. We conclude either that the CYCA1;2 produced in prophase I indirectly regulates meiosis II progression, or that a very low level of CYCA1;2 directly regulates meiosis II progression. Either of these scenarios is a deviation from the typical mode of action of mitotic cyclins in mitosis and meiosis I, in which each nuclear division is coupled with a peak of expression of mitotic cyclins.

² Present address: Lab. Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales, Université Jean-Monnet, 23, rue du Dr. Paul Michelon, 42023 Saint Etienne cedex 2, France.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.051201.

^* Corresponding author; e-mail yming{at}okstate.edu; fax 405–744–7074.

Received August 4, 2004; returned for revision October 26, 2004; accepted October 26, 2004.

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