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First published online November 19, 2004; 10.1104/pp.104.052142

Plant Physiology 136:4159-4168 (2004)
© 2004 American Society of Plant Biologists

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ENVIRONMENTAL STRESS AND ADAPTATION

Exploring the Temperature-Stress Metabolome of Arabidopsis1,[w]

Fatma Kaplan, Joachim Kopka, Dale W. Haskell, Wei Zhao, K. Cameron Schiller, Nicole Gatzke, Dong Yul Sung2 and Charles L. Guy*

Plant Molecular and Cellular Biology Program, Environmental Horticulture (F.K., D.W.H., D.Y.S., C.L.G.), Department of Statistics (W.Z.), and Pharmacy Health Care Administration (K.C.S.), University of Florida, Gainesville, Florida 32611; and Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany (J.K., N.G.)

Metabolic profiling analyses were performed to determine metabolite temporal dynamics associated with the induction of acquired thermotolerance in response to heat shock and acquired freezing tolerance in response to cold shock. Low-Mr polar metabolite analyses were performed using gas chromatography-mass spectrometry. Eighty-one identified metabolites and 416 unidentified mass spectral tags, characterized by retention time indices and specific mass fragments, were monitored. Cold shock influenced metabolism far more profoundly than heat shock. The steady-state pool sizes of 143 and 311 metabolites or mass spectral tags were altered in response to heat and cold shock, respectively. Comparison of heat- and cold-shock response patterns revealed that the majority of heat-shock responses were shared with cold-shock responses, a previously unknown relationship. Coordinate increases in the pool sizes of amino acids derived from pyruvate and oxaloacetate, polyamine precursors, and compatible solutes were observed during both heat and cold shock. In addition, many of the metabolites that showed increases in response to both heat and cold shock in this study were previously unlinked with temperature stress. This investigation provides new insight into the mechanisms of plant adaptation to thermal stress at the metabolite level, reveals relationships between heat- and cold-shock responses, and highlights the roles of known signaling molecules and protectants.

1 This work was supported by National Aeronautics and Space Administration (grant no. NAG10–316), by the U.S. Department of Agriculture (National Research Initiative grant nos. 2000–35100–9532 and 2002–35100–12110), and by the Institute of Food and Agricultural Sciences at the University of Florida. This article is Journal Series Number R–10483.

2 Present address: Division of Biological Sciences, University of California, San Diego, CA 92093.

[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.052142.

* Corresponding author; e-mail clguy{at}ufl.edu; fax 352–392–1413.

Received August 23, 2004; returned for revision October 5, 2004; accepted October 5, 2004.


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