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BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Organization of Monoterpene Biosynthesis in Mentha. Immunocytochemical Localizations of Geranyl Diphosphate Synthase, Limonene-6-Hydroxylase, Isopiperitenol Dehydrogenase, and Pulegone Reductase¹

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164

We present immunocytochemical localizations of four enzymes involved in p-menthane monoterpene biosynthesis in mint: the large and small subunits of peppermint (Mentha x piperita) geranyl diphosphate synthase, spearmint (Mentha spicata) (–)-(4S)-limonene-6-hydroxylase, peppermint (–)-trans-isopiperitenol dehydrogenase, and peppermint (+)-pulegone reductase. All were localized to the secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development. Immunogold labeling of geranyl diphosphate synthase occurred within secretory cell leucoplasts, (–)-4S-limonene-6-hydroxylase labeling was associated with gland cell endoplasmic reticulum, (–)-trans-isopiperitenol dehydrogenase labeling was restricted to secretory cell mitochondria, while (+)-pulegone reductase labeling occurred only in secretory cell cytoplasm. We discuss this pathway compartmentalization in relation to possible mechanisms for the intracellular movement of monoterpene metabolites, and for monoterpene secretion into the extracellular essential oil storage cavity.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.050229.

^* Corresponding author; e-mail croteau{at}wsu.edu; fax 1–509–335–7643.

Received August 2, 2004; returned for revision September 28, 2004; accepted September 29, 2004.

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