Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online December 23, 2004; 10.1104/pp.104.053041

Plant Physiology 137:104-116 (2005)
© 2005 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
137/1/104    most recent
pp.104.053041v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Plant Physiol.
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (114)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Borner, G. H.H.
Right arrow Articles by Dupree, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Borner, G. H.H.
Right arrow Articles by Dupree, P.
Agricola
Right arrow Articles by Borner, G. H.H.
Right arrow Articles by Dupree, P.
CELL BIOLOGY AND SIGNAL TRANSDUCTION

Analysis of Detergent-Resistant Membranes in Arabidopsis. Evidence for Plasma Membrane Lipid Rafts1

Georg H.H. Borner2, D. Janine Sherrier3, Thilo Weimar, Louise V. Michaelson, Nathan D. Hawkins, Andrew MacAskill, Johnathan A. Napier, Michael H. Beale, Kathryn S. Lilley and Paul Dupree*

Department of Biochemistry (G.H.H.B., D.J.S., T.W., A.M., P.D.) and Cambridge Centre for Proteomics (P.D., K.S.L.), University of Cambridge, Cambridge CB2 1QW, United Kingdom; and Crop Performance and Improvement, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (L.V.M., N.D.H., J.A.N., M.H.B.)

The trafficking and function of cell surface proteins in eukaryotic cells may require association with detergent-resistant sphingolipid- and sterol-rich membrane domains. The aim of this work was to obtain evidence for lipid domain phenomena in plant membranes. A protocol to prepare Triton X-100 detergent-resistant membranes (DRMs) was developed using Arabidopsis (Arabidopsis thaliana) callus membranes. A comparative proteomics approach using two-dimensional difference gel electrophoresis and liquid chromatography-tandem mass spectrometry revealed that the DRMs were highly enriched in specific proteins. They included eight glycosylphosphatidylinositol-anchored proteins, several plasma membrane (PM) ATPases, multidrug resistance proteins, and proteins of the stomatin/prohibitin/hypersensitive response family, suggesting that the DRMs originated from PM domains. We also identified a plant homolog of flotillin, a major mammalian DRM protein, suggesting a conserved role for this protein in lipid domain phenomena in eukaryotic cells. Lipid analysis by gas chromatography-mass spectrometry showed that the DRMs had a 4-fold higher sterol-to-protein content than the average for Arabidopsis membranes. The DRMs were also 5-fold increased in sphingolipid-to-protein ratio. Our results indicate that the preparation of DRMs can yield a very specific set of membrane proteins and suggest that the PM contains phytosterol and sphingolipid-rich lipid domains with a specialized protein composition. Our results also suggest a conserved role of lipid modification in targeting proteins to both the intracellular and extracellular leaflet of these domains. The proteins associated with these domains provide important new experimental avenues into understanding plant cell polarity and cell surface processes.

1 This work was supported by the Biotechnology and Biological Sciences Research Council, by the Biotechnology and Biological Sciences Research Council Investigating Gene Function Initiative GARNet, by a European Community's Framework V Research Training Network Contract (HPRN–CT–2002–00262) Biointeractions (to T.W.), by the Nuffield Foundation, and by the Studienstiftung des Deutschen Volkes (scholarship to G.H.H.B.).

2 Present address: Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2XY, UK.

3 Present address: Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.053041.

* Corresponding author; e-mail p.dupree{at}bioc.cam.ac.uk; fax 44–1223–333345.

Received September 6, 2004; returned for revision October 17, 2004; accepted October 23, 2004.


Related articles in Plant Physiol.:

On the Inside
Peter V. Minorsky
Plant Physiol. 2005 137: 1-2. [Full Text]  



This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
M. Boehm, J. Nield, P. Zhang, E.-M. Aro, J. Komenda, and P. J. Nixon
Structural and Mutational Analysis of Band 7 Proteins in the Cyanobacterium Synechocystis sp. Strain PCC 6803
J. Bacteriol., October 15, 2009; 191(20): 6425 - 6435.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Pinheiro, M. Samalova, N. Geldner, J. Chory, A. Martinez, and I. Moore
Genetic evidence that the higher plant Rab-D1 and Rab-D2 GTPases exhibit distinct but overlapping interactions in the early secretory pathway
J. Cell Sci., October 15, 2009; 122(20): 3749 - 3758.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
R. A. Burton and G. B. Fincher
(1,3;1,4)-{beta}-D-Glucans in Cell Walls of the Poaceae, Lower Plants, and Fungi: A Tale of Two Linkages
Mol Plant, September 1, 2009; 2(5): 873 - 882.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
T. Stanislas, D. Bouyssie, M. Rossignol, S. Vesa, J. Fromentin, J. Morel, C. Pichereaux, B. Monsarrat, and F. Simon-Plas
Quantitative Proteomics Reveals a Dynamic Association of Proteins to Detergent-resistant Membranes upon Elicitor Signaling in Tobacco
Mol. Cell. Proteomics, September 1, 2009; 8(9): 2186 - 2198.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. A. Wimmer, G. Lochnit, E. Bassil, K. H. Muhling, and H. E. Goldbach
Membrane-Associated, Boron-Interacting Proteins Isolated by Boronate Affinity Chromatography
Plant Cell Physiol., July 1, 2009; 50(7): 1292 - 1304.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Beaudoin, X. Wu, F. Li, R. P. Haslam, J. E. Markham, H. Zheng, J. A. Napier, and L. Kunst
Functional Characterization of the Arabidopsis {beta}-Ketoacyl-Coenzyme A Reductase Candidates of the Fatty Acid Elongase
Plant Physiology, July 1, 2009; 150(3): 1174 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
Y. Z. Zheng, K. B. Berg, and L. J. Foster
Mitochondria do not contain lipid rafts, and lipid rafts do not contain mitochondrial proteins
J. Lipid Res., May 1, 2009; 50(5): 988 - 998.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Raffaele, E. Bayer, D. Lafarge, S. Cluzet, S. German Retana, T. Boubekeur, N. Leborgne-Castel, J.-P. Carde, J. Lherminier, E. Noirot, et al.
Remorin, a Solanaceae Protein Resident in Membrane Rafts and Plasmodesmata, Impairs Potato virus X Movement
PLANT CELL, May 1, 2009; 21(5): 1541 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. Briolay, J. Bouzenzana, M. Guichardant, C. Deshayes, N. Sindt, L. Bessueille, and V. Bulone
Cell Wall Polysaccharide Synthases Are Located in Detergent-Resistant Membrane Microdomains in Oomycetes
Appl. Envir. Microbiol., April 1, 2009; 75(7): 1938 - 1949.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
S. Kierszniowska, B. Seiwert, and W. X. Schulze
Definition of Arabidopsis Sterol-rich Membrane Microdomains by Differential Treatment with Methyl-{beta}-cyclodextrin and Quantitative Proteomics
Mol. Cell. Proteomics, April 1, 2009; 8(4): 612 - 623.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. DeBono, T. H. Yeats, J. K.C. Rose, D. Bird, R. Jetter, L. Kunst, and L. Samuels
Arabidopsis LTPG Is a Glycosylphosphatidylinositol-Anchored Lipid Transfer Protein Required for Export of Lipids to the Plant Surface
PLANT CELL, April 1, 2009; 21(4): 1230 - 1238.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. J. Reina-Pinto, D. Voisin, S. Kurdyukov, A. Faust, R. P. Haslam, L. V. Michaelson, N. Efremova, B. Franke, L. Schreiber, J. A. Napier, et al.
Misexpression of FATTY ACID ELONGATION1 in the Arabidopsis Epidermis Induces Cell Death and Suggests a Critical Role for Phospholipase A2 in This Process
PLANT CELL, April 1, 2009; 21(4): 1252 - 1272.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Titapiwatanakun and A. S. Murphy
Post-transcriptional regulation of auxin transport proteins: cellular trafficking, protein phosphorylation, protein maturation, ubiquitination, and membrane composition
J. Exp. Bot., March 1, 2009; 60(4): 1093 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Minami, M. Fujiwara, A. Furuto, Y. Fukao, T. Yamashita, M. Kamo, Y. Kawamura, and M. Uemura
Alterations in Detergent-Resistant Plasma Membrane Microdomains in Arabidopsis thaliana During Cold Acclimation
Plant Cell Physiol., February 1, 2009; 50(2): 341 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. V. Michaelson, S. Zauner, J. E. Markham, R. P. Haslam, R. Desikan, S. Mugford, S. Albrecht, D. Warnecke, P. Sperling, E. Heinz, et al.
Functional Characterization of a Higher Plant Sphingolipid {Delta}4-Desaturase: Defining the Role of Sphingosine and Sphingosine-1-Phosphate in Arabidopsis
Plant Physiology, January 1, 2009; 149(1): 487 - 498.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Zauner, U. Zahringer, B. Lindner, D. Warnecke, and P. Sperling
Identification and Functional Characterization of the 2-Hydroxy Fatty N-Acyl-{Delta}3(E)-desaturase from Fusarium graminearum
J. Biol. Chem., December 26, 2008; 283(52): 36734 - 36742.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
A. Driouich and T. I. Baskin
Intercourse between cell wall and cytoplasm exemplified by arabinogalactan proteins and cortical microtubules
Am. J. Botany, December 1, 2008; 95(12): 1491 - 1497.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S. Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas
Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts
FASEB J, November 1, 2008; 22(11): 3980 - 3991.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Klima and I. Foissner
FM Dyes Label Sterol-Rich Plasma Membrane Domains and are Internalized Independently of the Cytoskeleton in Characean Internodal Cells
Plant Cell Physiol., October 1, 2008; 49(10): 1508 - 1521.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
U. Krugel, L. M. Veenhoff, J. Langbein, E. Wiederhold, J. Liesche, T. Friedrich, B. Grimm, E. Martinoia, B. Poolman, and C. Kuhn
Transport and Sorting of the Solanum tuberosum Sucrose Transporter SUT1 Is Affected by Posttranslational Modification
PLANT CELL, September 1, 2008; 20(9): 2497 - 2513.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Onelli, C. Prescianotto-Baschong, M. Caccianiga, and A. Moscatelli
Clathrin-dependent and independent endocytic pathways in tobacco protoplasts revealed by labelling with charged nanogold
J. Exp. Bot., August 1, 2008; 59(11): 3051 - 3068.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Yalovsky, D. Bloch, N. Sorek, and B. Kost
Regulation of Membrane Trafficking, Cytoskeleton Dynamics, and Cell Polarity by ROP/RAC GTPases
Plant Physiology, August 1, 2008; 147(4): 1527 - 1543.
[Full Text] [PDF]

Home page
 Plant CellHome page
M. Chen, J. E. Markham, C. R. Dietrich, J. G. Jaworski, and E. B. Cahoon
Sphingolipid Long-Chain Base Hydroxylation Is Important for Growth and Regulation of Sphingolipid Content and Composition in Arabidopsis
PLANT CELL, July 1, 2008; 20(7): 1862 - 1878.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Mashiguchi, E. Urakami, M. Hasegawa, K. Sanmiya, I. Matsumoto, I. Yamaguchi, T. Asami, and Y. Suzuki
Defense-Related Signaling by Interaction of Arabinogalactan Proteins and {beta}-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cells
Plant Cell Physiol., February 1, 2008; 49(2): 178 - 190.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Marmagne, M. Ferro, T. Meinnel, C. Bruley, L. Kuhn, J. Garin, H. Barbier-Brygoo, and G. Ephritikhine
A High Content in Lipid-modified Peripheral Proteins and Integral Receptor Kinases Features in the Arabidopsis Plasma Membrane Proteome
Mol. Cell. Proteomics, November 1, 2007; 6(11): 1980 - 1996.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Raffaele, S. Mongrand, P. Gamas, A. Niebel, and T. Ott
Genome-Wide Annotation of Remorins, a Plant-Specific Protein Family: Evolutionary and Functional Perspectives
Plant Physiology, November 1, 2007; 145(3): 593 - 600.
[Full Text] [PDF]

Home page
 J Exp BotHome page
N. Takata, J. Kasuga, D. Takezawa, K. Arakawa, and S. Fujikawa
Gene expression associated with increased supercooling capability in xylem parenchyma cells of larch (Larix kaempferi)
J. Exp. Bot., October 1, 2007; 58(13): 3731 - 3742.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. R. Ryan, Q. Liu, P. Sperling, B. Dong, S. Franke, and E. Delhaize
A Higher Plant {Delta}8 Sphingolipid Desaturase with a Preference for (Z)-Isomer Formation Confers Aluminum Tolerance to Yeast and Plants
Plant Physiology, August 1, 2007; 144(4): 1968 - 1977.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Lefebvre, F. Furt, M.-A. Hartmann, L. V. Michaelson, J.-P. Carde, F. Sargueil-Boiron, M. Rossignol, J. A. Napier, J. Cullimore, J.-J. Bessoule, et al.
Characterization of Lipid Rafts from Medicago truncatula Root Plasma Membranes: A Proteomic Study Reveals the Presence of a Raft-Associated Redox System
Plant Physiology, May 1, 2007; 144(1): 402 - 418.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Sorek, L. Poraty, H. Sternberg, E. Bar, E. Lewinsohn, and S. Yalovsky
Activation Status-Coupled Transient S Acylation Determines Membrane Partitioning of a Plant Rho-Related GTPase
Mol. Cell. Biol., March 15, 2007; 27(6): 2144 - 2154.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. K. Grennan
Lipid Rafts in Plants
Plant Physiology, March 1, 2007; 143(3): 1083 - 1085.
[Full Text] [PDF]

Home page
 J Exp BotHome page
P. Moreau, F. Brandizzi, S. Hanton, L. Chatre, S. Melser, C. Hawes, and B. Satiat-Jeunemaitre
The plant ER-Golgi interface: a highly structured and dynamic membrane complex
J. Exp. Bot., January 1, 2007; 58(1): 49 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Laloi, A.-M. Perret, L. Chatre, S. Melser, C. Cantrel, M.-N. Vaultier, A. Zachowski, K. Bathany, J.-M. Schmitter, M. Vallet, et al.
Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells
Plant Physiology, January 1, 2007; 143(1): 461 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Chen, G. Han, C. R. Dietrich, T. M. Dunn, and E. B. Cahoon
The Essential Nature of Sphingolipids in Plants as Revealed by the Functional Identification and Characterization of the Arabidopsis LCB1 Subunit of Serine Palmitoyltransferase
PLANT CELL, December 1, 2006; 18(12): 3576 - 3593.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. S. Sardar, J. Yang, and A. M. Showalter
Molecular Interactions of Arabinogalactan Proteins with Cortical Microtubules and F-Actin in Bright Yellow-2 Tobacco Cultured Cells
Plant Physiology, December 1, 2006; 142(4): 1469 - 1479.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. E. Markham, J. Li, E. B. Cahoon, and J. G. Jaworski
Separation and Identification of Major Plant Sphingolipid Classes from Leaves
J. Biol. Chem., August 11, 2006; 281(32): 22684 - 22694.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
C. J. Nelson, A. D. Hegeman, A. C. Harms, and M. R. Sussman
A Quantitative Analysis of Arabidopsis Plasma Membrane Using Trypsin-catalyzed 18O Labeling
Mol. Cell. Proteomics, August 1, 2006; 5(8): 1382 - 1395.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. Morel, S. Claverol, S. Mongrand, F. Furt, J. Fromentin, J.-J. Bessoule, J.-P. Blein, and F. Simon-Plas
Proteomics of Plant Detergent-resistant Membranes
Mol. Cell. Proteomics, August 1, 2006; 5(8): 1396 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
G. Grossmann, M. Opekarova, L. Novakova, J. Stolz, and W. Tanner
Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae
Eukaryot. Cell, June 1, 2006; 5(6): 945 - 953.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. S Lilley and P. Dupree
Methods of quantitative proteomics and their application to plant organelle characterization
J. Exp. Bot., April 1, 2006; 57(7): 1493 - 1499.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Baginsky and W. Gruissem
Arabidopsis thaliana proteomics: from proteome to genome
J. Exp. Bot., April 1, 2006; 57(7): 1485 - 1491.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-U. Sutter, P. Campanoni, M. Tyrrell, and M. R. Blatt
Selective Mobility and Sensitivity to SNAREs Is Exhibited by the Arabidopsis KAT1 K+ Channel at the Plasma Membrane
PLANT CELL, April 1, 2006; 18(4): 935 - 954.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Terasaka, J. J. Blakeslee, B. Titapiwatanakun, W. A. Peer, A. Bandyopadhyay, S. N. Makam, O. R. Lee, E. L. Richards, A. S. Murphy, F. Sato, et al.
PGP4, an ATP Binding Cassette P-Glycoprotein, Catalyzes Auxin Transport in Arabidopsis thaliana Roots
PLANT CELL, November 1, 2005; 17(11): 2922 - 2939.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Roudier, A. G. Fernandez, M. Fujita, R. Himmelspach, G. H.H. Borner, G. Schindelman, S. Song, T. I. Baskin, P. Dupree, G. O. Wasteneys, et al.
COBRA, an Arabidopsis Extracellular Glycosyl-Phosphatidyl Inositol-Anchored Protein, Specifically Controls Highly Anisotropic Expansion through Its Involvement in Cellulose Microfibril Orientation
PLANT CELL, June 1, 2005; 17(6): 1749 - 1763.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Zheng, O. Rowland, and L. Kunst
Disruptions of the Arabidopsis Enoyl-CoA Reductase Gene Reveal an Essential Role for Very-Long-Chain Fatty Acid Synthesis in Cell Expansion during Plant Morphogenesis
PLANT CELL, May 1, 2005; 17(5): 1467 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. S. Gillmor, W. Lukowitz, G. Brininstool, J. C. Sedbrook, T. Hamann, P. Poindexter, and C. Somerville
Glycosylphosphatidylinositol-Anchored Proteins Are Required for Cell Wall Synthesis and Morphogenesis in Arabidopsis
PLANT CELL, April 1, 2005; 17(4): 1128 - 1140.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists