First published online December 3, 2004; 10.1104/pp.104.054395
Plant Physiology 137:253-262 (2005)
© 2005 American Society of Plant Biologists
BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES
The Arabidopsis ATR1 Myb Transcription Factor Controls Indolic Glucosinolate Homeostasis1
John L. Celenza,
Juan A. Quiel,
Gromoslaw A. Smolen,
Houra Merrikh,
Angela R. Silvestro,
Jennifer Normanly and
Judith Bender*
Department of Biology, Boston University, Boston, Massachusetts 02215 (J.L.C., H.M., A.R.S.); Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205 (J.A.Q., G.A.S., J.B.); and Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003 (J.N.)
Plants derive a number of important secondary metabolites from the amino acid tryptophan (Trp), including the growth regulator indole-3-acetic acid (IAA) and defense compounds against pathogens and herbivores. In previous work, we found that a dominant overexpression allele of the Arabidopsis (Arabidopsis thaliana) Myb transcription factor ATR1, atr1D, activates expression of a Trp synthesis gene as well as the Trp-metabolizing genes CYP79B2, CYP79B3, and CYP83B1, which encode enzymes implicated in production of IAA and indolic glucosinolate (IG) antiherbivore compounds. Here, we show that ATR1 overexpression confers elevated levels of IAA and IGs. In addition, we show that an atr1 loss-of-function mutation impairs expression of IG synthesis genes and confers reduced IG levels. Furthermore, the atr1-defective mutation suppresses Trp gene dysregulation in a cyp83B1 mutant background. Together, this work implicates ATR1 as a key homeostatic regulator of Trp metabolism and suggests that ATR1 can be manipulated to coordinately control the suite of enzymes that synthesize IGs.
1 This work was supported by the National Science Foundation (grant nos. IBN–9723172 to J.B. and DBI–0077769 to J.L.C. and J.N.) and the U.S. Department of Agriculture (grant no. USDA 2002–35318–12715 to J.L.C. and J.N.).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.054395.
* Corresponding author; e-mail jbender{at}mail.jhmi.edu; fax 410–955–2926.
Received October 5, 2004;
returned for revision October 28, 2004;
accepted October 28, 2004.
This article has been cited by other articles:
|
|
|
|
 
S. Van der Ent, B. W.M. Verhagen, R. Van Doorn, D. Bakker, M. G. Verlaan, M. J.C. Pel, R. G. Joosten, M. C.G. Proveniers, L.C. Van Loon, J. Ton, et al.
MYB72 Is Required in Early Signaling Steps of Rhizobacteria-Induced Systemic Resistance in Arabidopsis
Plant Physiology,
March 1, 2008;
146(3):
1293 - 1304.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Brady, D. A. Orlando, J.-Y. Lee, J. Y. Wang, J. Koch, J. R. Dinneny, D. Mace, U. Ohler, and P. N. Benfey
A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns
Science,
November 2, 2007;
318(5851):
801 - 806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Dombrecht, G. P. Xue, S. J. Sprague, J. A. Kirkegaard, J. J. Ross, J. B. Reid, G. P. Fitt, N. Sewelam, P. M. Schenk, J. M. Manners, et al.
MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis
PLANT CELL,
July 1, 2007;
19(7):
2225 - 2245.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Y. Hirai, K. Sugiyama, Y. Sawada, T. Tohge, T. Obayashi, A. Suzuki, R. Araki, N. Sakurai, H. Suzuki, K. Aoki, et al.
Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis
PNAS,
April 10, 2007;
104(15):
6478 - 6483.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Hernandez-Blanco, D. X. Feng, J. Hu, A. Sanchez-Vallet, L. Deslandes, F. Llorente, M. Berrocal-Lobo, H. Keller, X. Barlet, C. Sanchez-Rodriguez, et al.
Impairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease Resistance
PLANT CELL,
March 1, 2007;
19(3):
890 - 903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Maruyama-Nakashita, Y. Nakamura, T. Tohge, K. Saito, and H. Takahashi
Arabidopsis SLIM1 Is a Central Transcriptional Regulator of Plant Sulfur Response and Metabolism
PLANT CELL,
November 1, 2006;
18(11):
3235 - 3251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. E. Blasing, Y. Gibon, M. Gunther, M. Hohne, R. Morcuende, D. Osuna, O. Thimm, B. Usadel, W.-R. Scheible, and M. Stitt
Sugars and Circadian Regulation Make Major Contributions to the Global Regulation of Diurnal Gene Expression in Arabidopsis
PLANT CELL,
December 1, 2005;
17(12):
3257 - 3281.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Jorgensen, S. Bak, P. K. Busk, C. Sorensen, C. E. Olsen, J. Puonti-Kaerlas, and B. L. Moller
Cassava Plants with a Depleted Cyanogenic Glucoside Content in Leaves and Tubers. Distribution of Cyanogenic Glucosides, Their Site of Synthesis and Transport, and Blockage of the Biosynthesis by RNA Interference Technology
Plant Physiology,
September 1, 2005;
139(1):
363 - 374.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ljung, A. K. Hull, J. Celenza, M. Yamada, M. Estelle, J. Normanly, and G. Sandberg
Sites and Regulation of Auxin Biosynthesis in Arabidopsis Roots
PLANT CELL,
April 1, 2005;
17(4):
1090 - 1104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
