Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online January 21, 2005; 10.1104/pp.104.055566

Plant Physiology 137:611-622 (2005)
© 2005 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
137/2/611    most recent
pp.104.055566v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nunes-Nesi, A.
Right arrow Articles by Fernie, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nunes-Nesi, A.
Right arrow Articles by Fernie, A. R.
Agricola
Right arrow Articles by Nunes-Nesi, A.
Right arrow Articles by Fernie, A. R.
BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Enhanced Photosynthetic Performance and Growth as a Consequence of Decreasing Mitochondrial Malate Dehydrogenase Activity in Transgenic Tomato Plants1

Adriano Nunes-Nesi, Fernando Carrari, Anna Lytovchenko, Anna M.O. Smith, Marcelo Ehlers Loureiro, R. George Ratcliffe, Lee J. Sweetlove and Alisdair R. Fernie*

Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476 Golm, Germany (A.N.-N., F.C., A.L., A.R.F.); Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom (A.M.O.S., R.G.R., L.J.S.); and Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa–Minas Gerais, Brazil (M.E.L.)

Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the mitochondrial malate dehydrogenase gene in the antisense orientation and exhibiting reduced activity of this isoform of malate dehydrogenase show enhanced photosynthetic activity and aerial growth under atmospheric conditions (360 ppm CO2). In comparison to wild-type plants, carbon dioxide assimilation rates and total plant dry matter were up to 11% and 19% enhanced in the transgenics, when assessed on a whole-plant basis. Accumulation of carbohydrates and redox-related compounds such as ascorbate was also markedly elevated in the transgenics. Also increased in the transgenic plants was the capacity to use L-galactono-lactone, the terminal precursor of ascorbate biosynthesis, as a respiratory substrate. Experiments in which ascorbate was fed to isolated leaf discs also resulted in increased rates of photosynthesis providing strong indication for an ascorbate-mediated link between the energy-generating processes of respiration and photosynthesis. This report thus shows that the repression of this mitochondrially localized enzyme improves both carbon assimilation and aerial growth in a crop species.

1 This work was supported by the Max-Planck-Gesellschaft (to F.C., A.N.-N., A.L., and A.R.F.), by Conselho Nacional de Desenvolvimento Científico e Tecnológico (A.N.-N.), by Deutscher Akademischer Austauschdienst (A.N.-N.), and by the United Kingdom Biotechnology and Biological Sciences Research Council (A.M.O.S., R.G.R., and L.J.S.).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.055566.

* Corresponding author; e-mail fernie{at}mpimp-golm.mpg.de; fax 49–(0)331–5678408.

Received October 23, 2004; returned for revision December 3, 2004; accepted December 5, 2004.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
T. C.R. Williams, L. Miguet, S. K. Masakapalli, N. J. Kruger, L. J. Sweetlove, and R. G. Ratcliffe
Metabolic Network Fluxes in Heterotrophic Arabidopsis Cells: Stability of the Flux Distribution under Different Oxygenation Conditions
Plant Physiology, October 1, 2008; 148(2): 704 - 718.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Fait, K. Hanhineva, R. Beleggia, N. Dai, I. Rogachev, V. J. Nikiforova, A. R. Fernie, and A. Aharoni
Reconfiguration of the Achene and Receptacle Metabolic Networks during Strawberry Fruit Development
Plant Physiology, October 1, 2008; 148(2): 730 - 750.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. B. Xu, T. Li, Z. Y. Deng, K. Chong, Y. Xue, and T. Wang
Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains
Plant Physiology, October 1, 2008; 148(2): 908 - 925.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
C. P. Lee, H. Eubel, N. O'Toole, and A. H. Millar
Heterogeneity of the Mitochondrial Proteome for Photosynthetic and Non-photosynthetic Arabidopsis Metabolism
Mol. Cell. Proteomics, July 1, 2008; 7(7): 1297 - 1316.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Bermudez, U. Urias, D. Milstein, L. Kamenetzky, R. Asis, A. R. Fernie, M. A. Van Sluys, F. Carrari, and M. Rossi
A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit
J. Exp. Bot., July 1, 2008; 59(10): 2875 - 2890.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Nunes-Nesi, R. Sulpice, Y. Gibon, and A. R. Fernie
The enigmatic contribution of mitochondrial function in photosynthesis
J. Exp. Bot., May 1, 2008; 59(7): 1675 - 1684.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. Morgan, M. Lehmann, M. Schwarzlander, C. J. Baxter, A. Sienkiewicz-Porzucek, T. C.R. Williams, N. Schauer, A. R. Fernie, M. D. Fricker, R. G. Ratcliffe, et al.
Decrease in Manganese Superoxide Dismutase Leads to Reduced Root Growth and Affects Tricarboxylic Acid Cycle Flux and Mitochondrial Redox Homeostasis
Plant Physiology, May 1, 2008; 147(1): 101 - 114.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Sienkiewicz-Porzucek, A. Nunes-Nesi, R. Sulpice, J. Lisec, D. C. Centeno, P. Carillo, A. Leisse, E. Urbanczyk-Wochniak, and A. R. Fernie
Mild Reductions in Mitochondrial Citrate Synthase Activity Result in a Compromised Nitrate Assimilation and Reduced Leaf Pigmentation But Have No Effect on Photosynthetic Performance or Growth
Plant Physiology, May 1, 2008; 147(1): 115 - 127.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. N. Oliver, A. Tiessen, A. R. Fernie, and P. Geigenberger
Decreased expression of plastidial adenylate kinase in potato tubers results in an enhanced rate of respiration and a stimulation of starch synthesis that is attributable to post-translational redox-activation of ADP-glucose pyrophosphorylase
J. Exp. Bot., February 4, 2008; (2008) erm312v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Alhagdow, F. Mounet, L. Gilbert, A. Nunes-Nesi, V. Garcia, D. Just, J. Petit, B. Beauvoit, A. R. Fernie, C. Rothan, et al.
Silencing of the Mitochondrial Ascorbate Synthesizing Enzyme L-Galactono-1,4-Lactone Dehydrogenase Affects Plant and Fruit Development in Tomato
Plant Physiology, December 1, 2007; 145(4): 1408 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Studart-Guimaraes, A. Fait, A. Nunes-Nesi, F. Carrari, B. Usadel, and A. R. Fernie
Reduced Expression of Succinyl-Coenzyme A Ligase Can Be Compensated for by Up-Regulation of the {gamma}-Aminobutyrate Shunt in Illuminated Tomato Leaves
Plant Physiology, November 1, 2007; 145(3): 626 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Lemaitre, E. Urbanczyk-Wochniak, V. Flesch, E. Bismuth, A. R. Fernie, and M. Hodges
NAD-Dependent Isocitrate Dehydrogenase Mutants of Arabidopsis Suggest the Enzyme Is Not Limiting for Nitrogen Assimilation
Plant Physiology, July 1, 2007; 144(3): 1546 - 1558.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Leon, L. Holuigue, and X. Jordana
Mitochondrial Complex II Is Essential for Gametophyte Development in Arabidopsis
Plant Physiology, April 1, 2007; 143(4): 1534 - 1546.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Palmieri, R. Arrigoni, E. Blanco, F. Carrari, M. I. Zanor, C. Studart-Guimaraes, A. R. Fernie, and F. Palmieri
Molecular Identification of an Arabidopsis S-Adenosylmethionine Transporter. Analysis of Organ Distribution, Bacterial Expression, Reconstitution into Liposomes, and Functional Characterization
Plant Physiology, November 1, 2006; 142(3): 855 - 865.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Carrari and A. R. Fernie
Metabolic regulation underlying tomato fruit development
J. Exp. Bot., June 1, 2006; 57(9): 1883 - 1897.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Jiang, T. Ballinger, D. Li, S. Zhang, and L. Feldman
A Role for Mitochondria in the Establishment and Maintenance of the Maize Root Quiescent Center
Plant Physiology, March 1, 2006; 140(3): 1118 - 1125.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Scheibe, J. E. Backhausen, V. Emmerlich, and S. Holtgrefe
Strategies to maintain redox homeostasis during photosynthesis under changing conditions
J. Exp. Bot., June 1, 2005; 56(416): 1481 - 1489.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists