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ENVIRONMENTAL STRESS AND ADAPTATION

A Genome-Wide Analysis of the Effects of Sucrose on Gene Expression in Arabidopsis Seedlings under Anoxia^[w]

Institute of Biology and Agricultural Biotechnology, Consiglio Nazionale delle Ricerche, 56124 Pisa, Italy (E.L.); Department of Crop Plant Biology (A.P., G.N., A.A.) and Research Center of Excellence for the Study of Damages to the Nervous and Endocrine Systems Produced by Environmental, Alimentary and Pharmacological Agents (A.A., P.P.), University of Pisa, 56124 Pisa, Italy; and Sant'Anna School of Advanced Studies, 56127 Pisa, Italy (P.P.)

Exogenous sucrose (Suc) greatly enhances anoxia tolerance of Arabidopsis (Arabidopsis thaliana) seedlings. We used the Affymetrix ATH1 GeneChip containing more than 22,500 probe sets to explore the anaerobic transcriptome of Arabidopsis seedlings kept under anoxia for 6 h in presence or absence of exogenous Suc. Functional clustering was performed using the MapMan software. Besides the expected induction of genes encoding enzymes involved in Suc metabolism and alcoholic fermentation, a large number of genes not related to these pathways were affected by anoxia. Addition of exogenous Suc mitigated the effects of anoxia on auxin responsive genes that are repressed under oxygen deprivation. Anoxia-induced Suc synthases showed a lower induction in presence of exogenous Suc, suggesting that induction of these genes might be related to an anoxia-dependent sugar starvation. Anoxic induction of genes coding for heat shock proteins was much stronger in presence of exogenous Suc. Interestingly, a short heat treatment enhanced anoxia tolerance, suggesting that heat shock proteins may play a role in survival to low oxygen. These results provide insight into the effects of Suc on the anoxic transcriptome and provide a list of candidate genes that enhance anoxia tolerance of Suc-treated seedlings.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.057299.

^* Corresponding author; e-mail pperata{at}agr.unipi.it; fax 39–0502216532.

Received November 27, 2004; returned for revision December 20, 2004; accepted January 5, 2005.

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