This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 137/3/1147    most recent pp.104.056028v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (24) Citing Articles via Google Scholar Google Scholar Articles by Huang, Z. Articles by Whitham, S. A. Search for Related Content PubMed PubMed Citation Articles by Huang, Z. Articles by Whitham, S. A. Agricola Articles by Huang, Z. Articles by Whitham, S. A.

PLANTS INTERACTING WITH OTHER ORGANISMS

Salicylic Acid-Dependent Expression of Host Genes in Compatible Arabidopsis-Virus Interactions^1,[w]

Department of Plant Pathology, Iowa State University, Ames, Iowa 50011–1020 (Z.H., J.D.H., S.A.W); and Illumina, Incorporated, San Diego, California 92121 (J.M.Y., E.W.G., J.-B.F.)

Plant viruses elicit the expression of common sets of genes in susceptible hosts. Studies in Arabidopsis (Arabidopsis thaliana) and tomato (Lycopersicon esculentum) indicate that at least one-third of the genes induced in common by viruses have been previously associated with plant defense and stress responses. The genetic and molecular requirements for the induction of these stress and defense-related genes during compatible host-virus interactions were investigated with a panel of Arabidopsis mutant and transgenic plants defective in one or more defense signaling pathways. pad4, eds5, NahG, npr1, jar1, ein2, sid2, eds1, and wild-type Columbia-0 and Wassilewskija-2 plants were infected with two different viruses, cucumber mosaic virus and oilseed rape mosaic virus. Gene expression was assayed by a high-throughput fiber-optic bead array consisting of 388 genes and by RNA gel blots. These analyses demonstrated that, in compatible host-virus interactions, the expression of the majority of defense-related genes is induced by a salicylic acid-dependent, NPR1-independent signaling pathway with a few notable exceptions that did require NPR1. Interestingly, none of the mutant or transgenic plants showed enhanced susceptibility to either cucumber mosaic virus or oilseed rape mosaic virus based on both symptoms and virus accumulation. This observation is in contrast to the enhanced disease susceptibility phenotypes that these mutations or transgenes confer to some bacterial and fungal pathogens. These experimental results suggest that expression of many defense-related genes in compatible host plants might share components of signaling pathways involved in incompatible host-pathogen interactions, but their increased expression has no negative effect on viral infection.

^[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.056028.

^* Corresponding author; e-mail swhitham{at}iastate.edu; fax 515–294–9420.

Received November 4, 2004; returned for revision November 30, 2004; accepted November 30, 2004.

This article has been cited by other articles:

				X. Wang, S. P. Goregaoker, and J. N. Culver Interaction of the Tobacco Mosaic Virus Replicase Protein with a NAC Domain Transcription Factor Is Associated with the Suppression of Systemic Host Defenses J. Virol., October 1, 2009; 83(19): 9720 - 9730. [Abstract] [Full Text] [PDF]

				J. T. Ascencio-Ibanez, R. Sozzani, T.-J. Lee, T.-M. Chu, R. D. Wolfinger, R. Cella, and L. Hanley-Bowdoin Global Analysis of Arabidopsis Gene Expression Uncovers a Complex Array of Changes Impacting Pathogen Response and Cell Cycle during Geminivirus Infection Plant Physiology, September 1, 2008; 148(1): 436 - 454. [Abstract] [Full Text] [PDF]

				M. I. Elvira, M. M. Galdeano, P. Gilardi, I. Garcia-Luque, and M. T. Serra Proteomic analysis of pathogenesis-related proteins (PRs) induced by compatible and incompatible interactions of pepper mild mottle virus (PMMoV) in Capsicum chinense L3 plants J. Exp. Bot., April 1, 2008; 59(6): 1253 - 1265. [Abstract] [Full Text] [PDF]

				C Espinoza, C Medina, S Somerville, and P Arce-Johnson Senescence-associated genes induced during compatible viral interactions with grapevine and Arabidopsis J. Exp. Bot., September 4, 2007; (2007) erm165v2. [Abstract] [Full Text] [PDF]