Plant Physiology 137:1272-1282 (2005)
© 2005 American Society of Plant Biologists
DEVELOPMENT AND HORMONE ACTION
Floral Patterning in Lotus japonicus1,[w]
Zhi-cheng Dong,
Zhong Zhao,
Cheng-wu Liu,
Jiang-hong Luo,
Jun Yang,
Wei-hua Huang,
Xiao-he Hu,
Trevor L. Wang and
Da Luo*
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences (Z.D., C.L., J.L., J.Y., W.H., X.H., D.L.), and Graduate School of the Chinese Academy of Sciences (Z.D., C.L., J.L.), Chinese Academy of Sciences, Shanghai 200032, China; School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China (D.L.); Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China (Z.Z.); and Department of Metabolic Biology, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom (T.L.W.)
Floral patterning in Papilionoideae plants, such as pea (Pisum sativum) and Medicago truncatula, is unique in terms of floral organ number, arrangement, and initiation timing as compared to other well-studied eudicots. To investigate the molecular mechanisms involved in the floral patterning in legumes, we have analyzed two mutants, proliferating floral meristem and proliferating floral organ-2 (pfo-2), obtained by ethyl methanesulfonate mutagenesis of Lotus japonicus. These two mutants showed similar phenotypes, with indeterminate floral structures and altered floral organ identities. We have demonstrated that loss of function of LjLFY and LjUFO/Pfo is likely to be responsible for these mutant phenotypes, respectively. To dissect the regulatory network controlling the floral patterning, we cloned homologs of the ABC function genes, which control floral organ identity in Arabidopsis (Arabidopsis thaliana). We found that some of the B and C function genes were duplicated. RNA in situ hybridization showed that the C function genes were expressed transiently in the carpel, continuously in stamens, and showed complementarity with the A function genes in the heterogeneous whorl. In proliferating floral meristem and pfo-2 mutants, all B function genes were down-regulated and the expression patterns of the A and C function genes were drastically altered. We conclude that LjLFY and LjUFO/Pfo are required for the activation of B function genes and function together in the recruitment and determination of petals and stamens. Our findings suggest that gene duplication, change in expression pattern, gain or loss of functional domains, and alteration of key gene functions all contribute to the divergence of floral patterning in L. japonicus.
1 This work was supported by the National High Technology Research and Development Program of China (grant no. 2003AA222030) and by the National Nature Science Foundation of China (grant nos. 30392100 and 30240018).
[w] The online version of this article contains Web-only data.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.054288.
* Corresponding author; e-mail dluo{at}sibs.ac.cn; fax 86–021–54924106.
Received October 13, 2004;
returned for revision February 2, 2005;
accepted February 5, 2005.
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