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WHOLE PLANT AND ECOPHYSIOLOGY

Dynamics of Exogenous Nitrogen Partitioning and Nitrogen Remobilization from Vegetative Organs in Pea Revealed by ¹⁵N in Vivo Labeling throughout Seed Filling¹

Unité de Génétique et d'Ecophysiologie des Légumineuses à Graines, Institut National de la Recherche Agronomique, 21065 Dijon cedex, France

The fluxes of (1) exogenous nitrogen (N) assimilation and (2) remobilization of endogenous N from vegetative plant compartments were measured by ¹⁵N labeling during the seed-filling period in pea (Pisum sativum L. cv Caméor), to better understand the mechanism of N remobilization. While the majority (86%) of exogenous N was allocated to the vegetative organs before the beginning of seed filling, this fraction decreased to 45% at the onset of seed filling, the remainder being directed to seeds. Nitrogen remobilization from vegetative parts contributed to 71% of the total N in mature seeds borne on the first two nodes (first stratum). The contribution of remobilized N to total seed N varied, with the highest proportion at the beginning of filling; it was independent of the developmental stage of each stratum of seeds, suggesting that remobilized N forms a unique pool, managed at the whole-plant level and supplied to all filling seeds whatever their position on the plant. Once seed filling starts, N is remobilized from all vegetative organs: 30% of the total N accumulated in seeds was remobilized from leaves, 20% from pod walls, 11% from roots, and 10% from stems. The rate of N remobilization was maximal when seeds of all the different strata were filling, consistent with regulation according to the N demand of seeds. At later stages of seed filling, the rate of remobilization decreases and may become controlled by the amount of residual N in vegetative tissues.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.056713.

^* Corresponding author; e-mail schiltz{at}epoisses.inra.fr; fax 33–3–80–69–32–22.

Received November 16, 2004; returned for revision December 19, 2004; accepted January 22, 2005.

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