First published online May 27, 2005; 10.1104/pp.104.058032
Plant Physiology 138:1083-1096 (2005)
© 2005 American Society of Plant Biologists
CELL BIOLOGY AND SIGNAL TRANSDUCTION
The Arabidopsis Transcription Factor MYB12 Is a Flavonol-Specific Regulator of Phenylpropanoid Biosynthesis1
Frank Mehrtens2,
Harald Kranz3,
Pawel Bednarek and
Bernd Weisshaar*
Max-Planck-Institute for Plant Breeding Research, D–50829 Cologne, Germany
Comprehensive functional data on plant R2R3-MYB transcription factors is still scarce compared to the manifold of their occurrence. Here, we identified the Arabidopsis (Arabidopsis thaliana) R2R3-MYB transcription factor MYB12 as a flavonol-specific activator of flavonoid biosynthesis. Transient expression in Arabidopsis protoplasts revealed a high degree of functional similarity between MYB12 and the structurally closely related factor P from maize (Zea mays). Both displayed similar target gene specificity, and both activated target gene promoters only in the presence of a functional MYB recognition element. The genes encoding the flavonoid biosynthesis enzymes chalcone synthase, chalcone flavanone isomerase, flavanone 3-hydroxylase, and flavonol synthase were identified as target genes. Hence, our observations further add to the general notion of a close relationship between structure and function of R2R3-MYB factors. High-performance liquid chromatography analyses of myb12 mutant plants and MYB12 overexpression plants demonstrate a tight linkage between the expression level of functional MYB12 and the flavonol content of young seedlings. Quantitative real time reverse transcription-PCR using these mutant plants showed MYB12 to be a transcriptional regulator of CHALCONE SYNTHASE and FLAVONOL SYNTHASE in planta, the gene products of which are indispensable for the biosynthesis of flavonols.
1 This work was supported by the Max-Planck-Society, and in part by EC-BIOTECH (grant no. BIO4–CT95–0129 to B.W.) and by the Deutsche Forschungsgemeinschaft.
2 Present address: Bielefeld University, Department of Biology, Genome Research, D–33594 Bielefeld, Germany.
3 Present address: Gene Bridges GmbH, D–69120 Heidelberg, Germany.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.058032.
* Corresponding author; e-mail bernd.weisshaar{at}uni-bielefeld.de; fax 49–521–1066423.
Received December 11, 2004;
returned for revision February 24, 2005;
accepted March 10, 2005.
This article has been cited by other articles:
|
|
|
|
 
R. A. E. Laitinen, M. Ainasoja, S. K. Broholm, T. H. Teeri, and P. Elomaa
Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida
J. Exp. Bot.,
August 25, 2008;
(2008)
ern216v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. K. Owens, A. B. Alerding, K. C. Crosby, A. B. Bandara, J. H. Westwood, and B. S.J. Winkel
Functional Analysis of a Predicted Flavonol Synthase Gene Family in Arabidopsis
Plant Physiology,
July 1, 2008;
147(3):
1046 - 1061.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Cloix and G. I. Jenkins
Interaction of the Arabidopsis UV-B-Specific Signaling Component UVR8 with Chromatin
Mol Plant,
January 1, 2008;
1(1):
118 - 128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-s. Su and J. C. Lagarias
Light-Independent Phytochrome Signaling Mediated by Dominant GAF Domain Tyrosine Mutants of Arabidopsis Phytochromes in Transgenic Plants
PLANT CELL,
July 1, 2007;
19(7):
2124 - 2139.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yonekura-Sakakibara, T. Tohge, R. Niida, and K. Saito
Identification of a Flavonol 7-O-Rhamnosyltransferase Gene Determining Flavonoid Pattern in Arabidopsis by Transcriptome Coexpression Analysis and Reverse Genetics
J. Biol. Chem.,
May 18, 2007;
282(20):
14932 - 14941.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lee, K. He, V. Stolc, H. Lee, P. Figueroa, Y. Gao, W. Tongprasit, H. Zhao, I. Lee, and X. W. Deng
Analysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of Development
PLANT CELL,
March 1, 2007;
19(3):
731 - 749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bogs, F. W. Jaffe, A. M. Takos, A. R. Walker, and S. P. Robinson
The Grapevine Transcription Factor VvMYBPA1 Regulates Proanthocyanidin Synthesis during Fruit Development
Plant Physiology,
March 1, 2007;
143(3):
1347 - 1361.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mano, F. Ogasawara, K. Sato, H. Higo, and Y. Minobe
Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet Potato
Plant Physiology,
March 1, 2007;
143(3):
1252 - 1268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Takos, F. W. Jaffe, S. R. Jacob, J. Bogs, S. P. Robinson, and A. R. Walker
Light-Induced Expression of a MYB Gene Regulates Anthocyanin Biosynthesis in Red Apples
Plant Physiology,
November 1, 2006;
142(3):
1216 - 1232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Oravecz, A. Baumann, Z. Mate, A. Brzezinska, J. Molinier, E. J. Oakeley, E. Adam, E. Schafer, F. Nagy, and R. Ulm
CONSTITUTIVELY PHOTOMORPHOGENIC1 Is Required for the UV-B Response in Arabidopsis
PLANT CELL,
August 1, 2006;
18(8):
1975 - 1990.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Morita, M. Saitoh, A. Hoshino, E. Nitasaka, and S. Iida
Isolation of cDNAs for R2R3-MYB, bHLH and WDR Transcriptional Regulators and Identification of c and ca Mutations Conferring White Flowers in the Japanese Morning Glory
Plant Cell Physiol.,
April 1, 2006;
47(4):
457 - 470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bogs, A. Ebadi, D. McDavid, and S. P. Robinson
Identification of the Flavonoid Hydroxylases from Grapevine and Their Regulation during Fruit Development
Plant Physiology,
January 1, 2006;
140(1):
279 - 291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
