This Article Full Text Full Text (PDF) All Versions of this Article: 138/2/870    most recent pp.105.060236v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Takahashi, S. Articles by Tanaka, A. Search for Related Content PubMed PubMed Citation Articles by Takahashi, S. Articles by Tanaka, A. Agricola Articles by Takahashi, S. Articles by Tanaka, A.

ENVIRONMENTAL STRESS AND ADAPTATION

Roles of Arabidopsis AtREV1 and AtREV7 in Translesion Synthesis¹

Department of Ion-beam-applied Biology, Japan Atomic Energy Research Institute, Watanuki 1233, Takasaki, Gunma, 370–1292, Japan (S. Takahashi, A.S., A.T.); and Kazusa DNA Research Institute, 2–6–7, Kazusa-Kamatari, Kisarazu, Chiba, 292–0812, Japan (S.S., T.K., S. Tabata)

Plants have mechanisms for repairing and tolerating detrimental effects by various DNA damaging agents. A tolerance pathway that has been predicted to be present in higher plants is translesion synthesis (TLS), which is catalyzed by polymerases. In Arabidopsis (Arabidopsis thaliana), however, the only gene known to be involved in TLS is the Arabidopsis homolog of REV3, AtREV3, which is a putative catalytic subunit of Arabidopsis DNA polymerase . A disrupted mutant of AtREV3, rev3, was previously found to be highly sensitive to ultraviolet-B (UV-B) and various DNA damaging agents. REV1 and REV7 are thought to be components of translesion synthesis in plants. In this study, we identified the Arabidopsis homologs of REV1 and REV7 (AtREV1 and AtREV7). Several mutants carrying disrupted AtREV1 and AtREV7 genes were isolated from Arabidopsis T-DNA-inserted lines. An AtREV1-disrupted mutant, rev1, was found to be moderately sensitive to UV-B and DNA cross-linkers. A rev1rev3 double mutant, like rev3, showed high sensitivity to UV-B, -rays, and DNA cross-linkers. An AtREV7-disrupted mutant, rev7, was possibly sensitive to cis-diamminedichloroplatinum(II), a kind of DNA cross-linker, but it was not sensitive to acute UV-B and -ray irradiation. On the other hand, the aerial growth of rev7, like the aerial growth of rev1 and rev3, was inhibited by long-term UV-B. These results suggest that a TLS mechanism exists in a higher plant and show that AtREV1 and AtREV7 have important roles in tolerating exposure to DNA-damaging agents.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.060236.

^* Corresponding author; e-mail staka{at}taka.jaeri.go.jp; fax 81–27–346–9688.

Received January 26, 2005; returned for revision February 28, 2005; accepted March 9, 2005.

This article has been cited by other articles:

				I-P. Chen, A. Mannuss, N. Orel, F. Heitzeberg, and H. Puchta A Homolog of ScRAD5 Is Involved in DNA Repair and Homologous Recombination in Arabidopsis Plant Physiology, April 1, 2008; 146(4): 1786 - 1796. [Abstract] [Full Text] [PDF]

				R. Wen, J. A. Torres-Acosta, L. Pastushok, X. Lai, L. Pelzer, H. Wang, and W. Xiao Arabidopsis UEV1D Promotes Lysine-63-Linked Polyubiquitination and Is Involved in DNA Damage Response PLANT CELL, January 1, 2008; 20(1): 213 - 227. [Abstract] [Full Text] [PDF]

				S. Takahashi, A. N. Sakamoto, A. Tanaka, and K. Shimizu AtREV1, a Y-Family DNA Polymerase in Arabidopsis, Has Deoxynucleotidyl Transferase Activity in Vitro Plant Physiology, November 1, 2007; 145(3): 1052 - 1060. [Abstract] [Full Text] [PDF]