This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 138/2/977    most recent pp.105.060558v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Plant Physiol. Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (23) Citing Articles via Google Scholar Google Scholar Articles by Tung, C.-W. Articles by Nasrallah, J. B. Search for Related Content PubMed PubMed Citation Articles by Tung, C.-W. Articles by Nasrallah, J. B. Agricola Articles by Tung, C.-W. Articles by Nasrallah, J. B.

GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Genome-Wide Identification of Genes Expressed in Arabidopsis Pistils Specifically along the Path of Pollen Tube Growth^1,[w]

Department of Plant Biology, Cornell University, Ithaca, New York 14853 (C.-W.T., M.E.N., J.B.N.); and Department of Biology, University of Scranton, Scranton, Pennsylvania 18510 (K.G.D.)

Plant reproductive development is dependent on successful pollen-pistil interactions. In crucifers, the pollen tube must breach the stigma surface and burrow through the extracellular matrix of the stigma epidermal cells and transmitting tract cells before reaching its ovule targets. The high degree of specificity in pollen-pistil interactions and the precision of directional pollen tube growth suggest that signals are continually being exchanged between pollen/pollen tubes and cells of the pistil that line their path. However, with few exceptions, little is known about the genes that control these interactions. The specialized functions of stigma epidermal cells and transmitting tract cells are likely to depend on the activity of genes expressed specifically in these cells. In order to identify these genes, we used the Arabidopsis (Arabidopsis thaliana) ATH1 microarray to compare the whole-genome transcriptional profiles of stigmas and ovaries isolated from wild-type Arabidopsis and from transgenic plants in which cells of the stigma epidermis and transmitting tract were specifically ablated by expression of a cellular toxin. Among the 23,000 genes represented on the array, we identified 115 and 34 genes predicted to be expressed specifically in the stigma epidermis and transmitting tract, respectively. Both gene sets were significantly enriched in predicted secreted proteins, including potential signaling components and proteins that might contribute to reinforcing, modifying, or remodeling the structure of the extracellular matrix during pollination. The possible role of these genes in compatible and incompatible pollen-pistil interactions is discussed.

^[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.060558.

^* Corresponding author; e-mail jbn2{at}cornell.edu; fax 607–255–5407.

Received February 1, 2005; returned for revision March 31, 2005; accepted April 4, 2005.

Related articles in Plant Physiol.:

On The Inside

Peter V. Minorsky
Plant Physiol. 2005 138: 571-572. [Full Text]  

This article has been cited by other articles:

				T. Tantikanjana, N. Rizvi, M. E. Nasrallah, and J. B. Nasrallah A Dual Role for the S-Locus Receptor Kinase in Self-Incompatibility and Pistil Development Revealed by an Arabidopsis rdr6 Mutation PLANT CELL, September 1, 2009; 21(9): 2642 - 2654. [Abstract] [Full Text] [PDF]

				A. C. Quiapim, M. S. Brito, L. A.S. Bernardes, I. daSilva, I. Malavazi, H. C. DePaoli, J. B. Molfetta-Machado, S. Giuliatti, G. H. Goldman, and M. H. S. Goldman Analysis of the Nicotiana tabacum Stigma/Style Transcriptome Reveals Gene Expression Differences between Wet and Dry Stigma Species Plant Physiology, March 1, 2009; 149(3): 1211 - 1230. [Abstract] [Full Text] [PDF]

				S. Han, L. Green, and D. J. Schnell The Signal Peptide Peptidase Is Required for Pollen Function in Arabidopsis Plant Physiology, March 1, 2009; 149(3): 1289 - 1301. [Abstract] [Full Text] [PDF]

				C. Cosio and C. Dunand Specific functions of individual class III peroxidase genes J. Exp. Bot., February 1, 2009; 60(2): 391 - 408. [Abstract] [Full Text] [PDF]

				T. L. Sage, K. Hristova-Sarkovski, V. Koehl, J. Lyew, V. Pontieri, P. Bernhardt, P. Weston, S. Bagha, and G. Chiu Transmitting tissue architecture in basal-relictual angiosperms: Implications for transmitting tissue origins Am. J. Botany, January 1, 2009; 96(1): 183 - 206. [Abstract] [Full Text] [PDF]

				S. Farage-Barhom, S. Burd, L. Sonego, R. Perl-Treves, and A. Lers Expression analysis of the BFN1 nuclease gene promoter during senescence, abscission, and programmed cell death-related processes J. Exp. Bot., September 1, 2008; 59(12): 3247 - 3258. [Abstract] [Full Text] [PDF]

				M. Alves-Ferreira, F. Wellmer, A. Banhara, V. Kumar, J. L. Riechmann, and E. M. Meyerowitz Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development Plant Physiology, November 1, 2007; 145(3): 747 - 762. [Abstract] [Full Text] [PDF]

				M. Li, W. Xu, W. Yang, Z. Kong, and Y. Xue Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice Plant Physiology, August 1, 2007; 144(4): 1797 - 1812. [Abstract] [Full Text] [PDF]

				S. M. Brady, T. A. Long, and P. N. Benfey Unraveling the dynamic transcriptome. PLANT CELL, September 1, 2006; 18(9): 2101 - 2111. [Full Text] [PDF]