Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online June 17, 2005; 10.1104/pp.104.059097

Plant Physiology 138:1409-1421 (2005)
© 2005 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
138/3/1409    most recent
pp.104.059097v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suzuki, I.
Right arrow Articles by Murata, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, I.
Right arrow Articles by Murata, N.
Agricola
Right arrow Articles by Suzuki, I.
Right arrow Articles by Murata, N.
ENVIRONMENTAL STRESS AND ADAPTATION

The Histidine Kinase Hik34 Is Involved in Thermotolerance by Regulating the Expression of Heat Shock Genes in Synechocystis1,[w]

Iwane Suzuki2,*, Yu Kanesaki, Hidenori Hayashi, John J. Hall, William J. Simon, Antoni R. Slabas and Norio Murata

Division of Cellular Regulation, National Institute for Basic Biology, Myodaiji, Okazaki 444–8585, Japan (I.S., Y.K., N.M.); Department of Biomechanics, Graduate University of Advanced Studies, Myodaiji, Okazaki 444–8585, Japan (I.S., N.M.); Satellite Venture Business Laboratory, Ehime University, Matsuyama 790–8577, Japan (Y.K., H.H.); and School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United Kingdom (J.J.H., W.J.S., A.R.S.)

Histidine kinases (Hiks) in Synechocystis sp. PCC 6803 are involved in the transduction of signals associated with various kinds of environmental stress. To examine the potential role in thermotolerance of Hiks, we used genome microarray analysis to screen a Hik knockout library for mutations that affected the expression of genes for heat shock proteins. Mutation of the hik34 gene enhanced the levels of transcripts of a number of heat shock genes, including htpG and groESL1. Overexpression of the hik34 gene repressed the expression of these heat shock genes. In addition, the cells with a mutant gene for Hik34 ({Delta}Hik34 cells) survived incubation at 48°C for 3 h, while wild-type cells and cells with mutations in other Hiks were killed. However, mutation of the hik34 gene had only an insignificant effect on the global expression of genes upon incubation of the mutant cells at 44°C for 20 min. Quantitative two-dimensional gel electrophoresis revealed that levels of GroES and HspA were elevated in {Delta}Hik34 cells after incubation of cells at 42°C for 60 min. We overexpressed recombinant Hik34 protein in Escherichia coli and purified it. We found that the protein was autophosphorylated in vitro at physiological temperatures, but not at elevated temperatures, such as 44°C. These results suggest that Hik34 might negatively regulate the expression of certain heat shock genes that might be related to thermotolerance in Synechocystis.

1 This work was supported by Grants-in-Aid for Scientific Research for Priority Areas (C) Genome Biology (grant no. 16013249 to I.S.) and for Priority Areas (grant no. 14086207 to N.M.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a Grant-in-Aid for Scientific Research (S; grant no. 13854002 to I.S. and N.M.) from the Japan Society for the Promotion of Science. This work was also supported by the Salt Science Research Foundation and by the National Institute for Basic Biology Cooperative Research Program on Stress-Tolerant Plants. A.R.S., J.J.H., and W.J.S. are supported by the Biotechnology and Biological Sciences Research Council (BBSRC). J.J.H. wishes to thank Amersham Biosciences, United Kingdom, for supporting a BBSRC Ph.D. Cooperative Awards in Science and Engineering studentship.

2 Present address: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba 305–8572, Japan.

[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.059097.

* Corresponding author; e-mail iwanes6803{at}biol.tsukuba.ac.jp; fax 81–29–853–4666.

Received December 28, 2004; returned for revision March 10, 2005; accepted March 22, 2005.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Sakayori, Y. Shiraiwa, and I. Suzuki
A Synechocystis Homolog of SipA Protein, Ssl3451, Enhances the Activity of the Histidine Kinase Hik33
Plant Cell Physiol., August 1, 2009; 50(8): 1439 - 1448.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
R. Vidal, L. Lopez-Maury, M. G. Guerrero, and F. J. Florencio
Characterization of an Alcohol Dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 That Responds to Environmental Stress Conditions via the Hik34-Rre1 Two-Component System
J. Bacteriol., July 1, 2009; 191(13): 4383 - 4391.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. Oliveira and P. Lindblad
An AbrB-Like Protein Regulates the Expression of the Bidirectional Hydrogenase in Synechocystis sp. Strain PCC 6803
J. Bacteriol., February 1, 2008; 190(3): 1011 - 1019.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
A. Higo, T. Suzuki, M. Ikeuchi, and M. Ohmori
Dynamic transcriptional changes in response to rehydration in Anabaena sp. PCC 7120
Microbiology, November 1, 2007; 153(11): 3685 - 3694.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Lee, J.-Y. Ryu, S. Y. Kim, J.-H. Jeon, J. Y. Song, H.-T. Cho, S.-B. Choi, D. Choi, N. T. de Marsac, and Y.-I. Park
Transcriptional Regulation of the Respiratory Genes in the Cyanobacterium Synechocystis sp. PCC 6803 during the Early Response to Glucose Feeding
Plant Physiology, November 1, 2007; 145(3): 1018 - 1030.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Sato, K. Nimura-Matsune, S. Watanabe, T. Chibazakura, and H. Yoshikawa
Expression Analysis of Multiple dnaK Genes in the Cyanobacterium Synechococcus elongatus PCC 7942
J. Bacteriol., May 15, 2007; 189(10): 3751 - 3758.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. K. Ashby and J. Houmard
Cyanobacterial Two-Component Proteins: Structure, Diversity, Distribution, and Evolution
Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 472 - 509.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists