Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online June 24, 2005; 10.1104/pp.105.059907

Plant Physiology 138:1516-1526 (2005)
© 2005 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
138/3/1516    most recent
pp.105.059907v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Montillet, J.-L.
Right arrow Articles by Triantaphylidès, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Montillet, J.-L.
Right arrow Articles by Triantaphylidès, C.
Agricola
Right arrow Articles by Montillet, J.-L.
Right arrow Articles by Triantaphylidès, C.
Related Collections
Right arrow Reactive Oxygen Species
PLANTS INTERACTING WITH OTHER ORGANISMS

Fatty Acid Hydroperoxides and H2O2 in the Execution of Hypersensitive Cell Death in Tobacco Leaves1,[w]

Jean-Luc Montillet, Sangpen Chamnongpol2, Christine Rustérucci3, James Dat4, Brigitte van de Cotte, Jean-Pierre Agnel, Christine Battesti, Dirk Inzé, Frank Van Breusegem and Christian Triantaphylidès*

Commissariat à l'Energie Atomique/Cadarache, Direction des Sciences du Vivant, Département d'Ecophysiologie Végétale et de Microbiologie, Laboratoire de Radiobiologie Végétale, F–13108 Saint-Paul-Lez-Durance cedex, France (J.-L.M., C.R., J.-P.A., C.B., C.T.); and Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B–9052 Ghent, Belgium (S.C., J.D., B.v.d.C., D.I., F.V.B.)

We initially compared lipid peroxidation profiles in tobacco (Nicotiana tabacum) leaves during different cell death events. An upstream oxylipin assay was used to discriminate reactive oxygen species (ROS)-mediated lipid peroxidation from 9- and 13-lipoxygenase (LOX)-dependent lipid peroxidation. Free radical-mediated membrane peroxidation was measured during H2O2-dependent cell death in leaves of catalase-deficient plants. Taking advantage of these transgenic plants, we demonstrate that, under light conditions, H2O2 plays an essential role in the execution of cell death triggered by an elicitor, cryptogein, which provokes a similar ROS-mediated lipid peroxidation. Under dark conditions, however, cell death induction by cryptogein was independent of H2O2 and accompanied by products of the 9-LOX pathway. In the hypersensitive response induced by the avirulent pathogen Pseudomonas syringae pv syringae, both 9-LOX and oxidative processes operated concurrently, with ROS-mediated lipid peroxidation prevailing in the light. Our results demonstrate, therefore, the tight interplay between H2O2 and lipid hydroperoxides and underscore the importance of light during the hypersensitive response.

1 This work was supported by the Research Fund of the Ghent University (Geconcerteerde Onderzoeksacties; grant no. 12051403) and by an individual Marie Curie postdoctoral fellowship of the European Union (to J.D.).

2 Present address: Panomics, Inc., 2003 East Bayshore Road, Redwood City, CA 94063.

3 Present address: Laboratoire de Génomique Fonctionnelle des Plantes, Faculté des Sciences–Ilot des Poulies, 33 Rue St. Leu, F–80039 Amiens cedex, France.

4 Present address: Laboratoire de Biologie Environnementale EA3184-usc, Institut National de la Recherche Agronomique, Université de Franche-Comté, Place Leclerc, F–25030 Besançon, France.

[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.059907.

* Corresponding author; e-mail ctriantaphylid{at}cea.fr; fax 33–4–42–25–26–25.

Received January 19, 2005; returned for revision April 13, 2005; accepted April 25, 2005.




This article has been cited by other articles:


Home page
 Plant CellHome page
S. Mueller, B. Hilbert, K. Dueckershoff, T. Roitsch, M. Krischke, M. J. Mueller, and S. Berger
General Detoxification and Stress Responses Are Mediated by Oxidized Lipids through TGA Transcription Factors in Arabidopsis
PLANT CELL, March 1, 2008; 20(3): 768 - 785.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. A. J. Mur, P. Kenton, A. J. Lloyd, H. Ougham, and E. Prats
The hypersensitive response; the centenary is upon us but how much do we know?
J. Exp. Bot., February 1, 2008; 59(3): 501 - 520.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Fammartino, F. Cardinale, C. Gobel, L. Mene-Saffrane, J. Fournier, I. Feussner, and M.-T. Esquerre-Tugaye
Characterization of a Divinyl Ether Biosynthetic Pathway Specifically Associated with Pathogenesis in Tobacco
Plant Physiology, January 1, 2007; 143(1): 378 - 388.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Danon, N. Sanchez Coll, and K. Apel
Cryptochrome-1-dependent execution of programmed cell death induced by singlet oxygen in Arabidopsis thaliana
PNAS, November 7, 2006; 103(45): 17036 - 17041.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. X. Andersson, M. Hamberg, O. Kourtchenko, A. Brunnstrom, K. L. McPhail, W. H. Gerwick, C. Gobel, I. Feussner, and M. Ellerstrom
Oxylipin Profiling of the Hypersensitive Response in Arabidopsis thaliana: FORMATION OF A NOVEL OXO-PHYTODIENOIC ACID-CONTAINING GALACTOLIPID, ARABIDOPSIDE E
J. Biol. Chem., October 20, 2006; 281(42): 31528 - 31537.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Torres, J. D.G. Jones, and J. L. Dangl
Reactive Oxygen Species Signaling in Response to Pathogens
Plant Physiology, June 1, 2006; 141(2): 373 - 378.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Zaninotto, S. L. Camera, A. Polverari, and M. Delledonne
Cross Talk between Reactive Nitrogen and Oxygen Species during the Hypersensitive Disease Resistance Response
Plant Physiology, June 1, 2006; 141(2): 379 - 383.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Van Breusegem and J. F. Dat
Reactive Oxygen Species in Plant Cell Death
Plant Physiology, June 1, 2006; 141(2): 384 - 390.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Zago, S. Morsa, J. F. Dat, P. Alard, A. Ferrarini, D. Inze, M. Delledonne, and F. Van Breusegem
Nitric Oxide- and Hydrogen Peroxide-Responsive Gene Regulation during Cell Death Induction in Tobacco
Plant Physiology, June 1, 2006; 141(2): 404 - 411.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Davoine, O. Falletti, T. Douki, G. Iacazio, N. Ennar, J.-L. Montillet, and C. Triantaphylides
Adducts of Oxylipin Electrophiles to Glutathione Reflect a 13 Specificity of the Downstream Lipoxygenase Pathway in the Tobacco Hypersensitive Response
Plant Physiology, April 1, 2006; 140(4): 1484 - 1493.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. A. J. Mur, T. L. W. Carver, and E. Prats
NO way to live; the various roles of nitric oxide in plant-pathogen interactions
J. Exp. Bot., February 1, 2006; 57(3): 489 - 505.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists