First published online June 24, 2005; 10.1104/pp.105.059907
Plant Physiology 138:1516-1526 (2005)
© 2005 American Society of Plant Biologists
PLANTS INTERACTING WITH OTHER ORGANISMS
Fatty Acid Hydroperoxides and H2O2 in the Execution of Hypersensitive Cell Death in Tobacco Leaves1,[w]
Jean-Luc Montillet,
Sangpen Chamnongpol2,
Christine Rustérucci3,
James Dat4,
Brigitte van de Cotte,
Jean-Pierre Agnel,
Christine Battesti,
Dirk Inzé,
Frank Van Breusegem and
Christian Triantaphylidès*
Commissariat à l'Energie Atomique/Cadarache, Direction des Sciences du Vivant, Département d'Ecophysiologie Végétale et de Microbiologie, Laboratoire de Radiobiologie Végétale, F–13108 Saint-Paul-Lez-Durance cedex, France (J.-L.M., C.R., J.-P.A., C.B., C.T.); and Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B–9052 Ghent, Belgium (S.C., J.D., B.v.d.C., D.I., F.V.B.)
We initially compared lipid peroxidation profiles in tobacco (Nicotiana tabacum) leaves during different cell death events. An upstream oxylipin assay was used to discriminate reactive oxygen species (ROS)-mediated lipid peroxidation from 9- and 13-lipoxygenase (LOX)-dependent lipid peroxidation. Free radical-mediated membrane peroxidation was measured during H2O2-dependent cell death in leaves of catalase-deficient plants. Taking advantage of these transgenic plants, we demonstrate that, under light conditions, H2O2 plays an essential role in the execution of cell death triggered by an elicitor, cryptogein, which provokes a similar ROS-mediated lipid peroxidation. Under dark conditions, however, cell death induction by cryptogein was independent of H2O2 and accompanied by products of the 9-LOX pathway. In the hypersensitive response induced by the avirulent pathogen Pseudomonas syringae pv syringae, both 9-LOX and oxidative processes operated concurrently, with ROS-mediated lipid peroxidation prevailing in the light. Our results demonstrate, therefore, the tight interplay between H2O2 and lipid hydroperoxides and underscore the importance of light during the hypersensitive response.
1 This work was supported by the Research Fund of the Ghent University (Geconcerteerde Onderzoeksacties; grant no. 12051403) and by an individual Marie Curie postdoctoral fellowship of the European Union (to J.D.).
2 Present address: Panomics, Inc., 2003 East Bayshore Road, Redwood City, CA 94063.
3 Present address: Laboratoire de Génomique Fonctionnelle des Plantes, Faculté des Sciences–Ilot des Poulies, 33 Rue St. Leu, F–80039 Amiens cedex, France.
4 Present address: Laboratoire de Biologie Environnementale EA3184-usc, Institut National de la Recherche Agronomique, Université de Franche-Comté, Place Leclerc, F–25030 Besançon, France.
[w] The online version of this article contains Web-only data.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.059907.
* Corresponding author; e-mail ctriantaphylid{at}cea.fr; fax 33–4–42–25–26–25.
Received January 19, 2005;
returned for revision April 13, 2005;
accepted April 25, 2005.
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