First published online June 24, 2005; 10.1104/pp.105.062174
Plant Physiology 138:1637-1643 (2005)
© 2005 American Society of Plant Biologists
CELL BIOLOGY AND SIGNAL TRANSDUCTION
The roothairless1 Gene of Maize Encodes a Homolog of sec3, Which Is Involved in Polar Exocytosis1
Tsui-Jung Wen2,
Frank Hochholdinger2,
Michaela Sauer,
Wesley Bruce and
Patrick S. Schnable*
Department of Agronomy (T.-J.W., P.S.S.), and Center for Plant Genomics (P.S.S.), Iowa State University, Ames, Iowa 50011; Department of General Genetics, Center for Plant Molecular Biology, University of Tuebingen, 72076 Tuebingen, Germany (F.H., M.S.); and Pioneer Hi-Bred International, Johnston, Iowa 50131–1004 (W.B.)
The roothairless1 (rth1) mutant is impaired in root hair elongation and exhibits other growth abnormalities. Unicellular root hairs elongate via localized tip growth, a process mediated by polar exocytosis of secretory vesicles. We report here the cloning of the rth1 gene that encodes a sec3 homolog. In yeast (Saccharomyces cerevisiae) and mammals, sec3 is a subunit of the exocyst complex, which tethers exocytotic vesicles prior to their fusion. The cloning of the rth1 gene associates the homologs of exocyst subunits to an exocytotic process in plant development and supports the hypothesis that exocyst-like proteins are involved in plant exocytosis. Proteomic analyses identified four proteins that accumulate to different levels in wild-type and rth1 primary roots. The preferential accumulation in the rth1 mutant proteome of a negative regulator of the cell cycle (a prohibitin) may at least partially explain the delayed development and flowering of the rth1 mutant.
1 This work was supported by Pioneer Hi-Bred International (grant to P.S.S.) and by Hatch Act and State of Iowa funds. Research on root biology in F.H.'s laboratory is supported by the German Scientific Council (grant nos. HO1149/4, HO1149/6, and SFB446/B16 to F.H.).
2 These authors contributed equally to the paper.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.062174.
* Corresponding author; e-mail schnable{at}iastate.edu; fax 515–294–5256.
Received March 4, 2005;
returned for revision April 8, 2005;
accepted April 21, 2005.
This article has been cited by other articles:
|
|
|
|
 
M. A. Samuel, Y. T. Chong, K. E. Haasen, M. G. Aldea-Brydges, S. L. Stone, and D. R. Goring
Cellular Pathways Regulating Responses to Compatible and Self-Incompatible Pollen in Brassica and Arabidopsis Stigmas Intersect at Exo70A1, a Putative Component of the Exocyst Complex
PLANT CELL,
September 1, 2009;
21(9):
2655 - 2671.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Brechenmacher, J. Lee, S. Sachdev, Z. Song, T. H. N. Nguyen, T. Joshi, N. Oehrle, M. Libault, B. Mooney, D. Xu, et al.
Establishment of a Protein Reference Map for Soybean Root Hair Cells
Plant Physiology,
February 1, 2009;
149(2):
670 - 682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ischebeck, I. Stenzel, and I. Heilmann
Type B Phosphatidylinositol-4-Phosphate 5-Kinases Mediate Arabidopsis and Nicotiana tabacum Pollen Tube Growth by Regulating Apical Pectin Secretion
PLANT CELL,
December 1, 2008;
20(12):
3312 - 3330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Guermonprez, A. Smertenko, M.-T. Crosnier, M. Durandet, N. Vrielynck, P. Guerche, P. J. Hussey, B. Satiat-Jeunemaitre, and S. Bonhomme
The POK/AtVPS52 protein localizes to several distinct post-Golgi compartments in sporophytic and gametophytic cells
J. Exp. Bot.,
August 1, 2008;
59(11):
3087 - 3098.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Yalovsky, D. Bloch, N. Sorek, and B. Kost
Regulation of Membrane Trafficking, Cytoskeleton Dynamics, and Cell Polarity by ROP/RAC GTPases
Plant Physiology,
August 1, 2008;
147(4):
1527 - 1543.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Van Damme, D. Inze, and E. Russinova
Vesicle Trafficking during Somatic Cytokinesis
Plant Physiology,
August 1, 2008;
147(4):
1544 - 1552.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Young, H. E. McFarlane, M. G. Hahn, T. L. Western, G. W. Haughn, and A. L. Samuels
Analysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich Mucilage
PLANT CELL,
June 1, 2008;
20(6):
1623 - 1638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Hala, R. Cole, L. Synek, E. Drdova, T. Pecenkova, A. Nordheim, T. Lamkemeyer, J. Madlung, F. Hochholdinger, J. E. Fowler, et al.
An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco
PLANT CELL,
May 1, 2008;
20(5):
1330 - 1345.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Li, T.-J. Wen, and P. S. Schnable
Role of RAD51 in the Repair of MuDR-Induced Double-Strand Breaks in Maize (Zea mays L.)
Genetics,
January 1, 2008;
178(1):
57 - 66.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tuberosa, S. Salvi, S. Giuliani, M. C. Sanguineti, M. Bellotti, S. Conti, and P. Landi
Genome-wide Approaches to Investigate and Improve Maize Response to Drought
Crop Sci.,
December 18, 2007;
47(Supplement_3):
S-120 - S-141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kwasniewski and I. Szarejko
Molecular Cloning and Characterization of beta-Expansin Gene Related to Root Hair Formation in Barley
Plant Physiology,
July 1, 2006;
141(3):
1149 - 1158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Cole, L. Synek, V. Zarsky, and J. E. Fowler
SEC8, a Subunit of the Putative Arabidopsis Exocyst Complex, Facilitates Pollen Germination and Competitive Pollen Tube Growth
Plant Physiology,
August 1, 2005;
138(4):
2005 - 2018.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
