Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online July 29, 2005; 10.1104/pp.105.066019

Plant Physiology 138:1877-1895 (2005)
© 2005 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
138/4/1877    most recent
pp.105.066019v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (35)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ju, H.-J.
Right arrow Articles by Verchot-Lubicz, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ju, H.-J.
Right arrow Articles by Verchot-Lubicz, J.
Agricola
Right arrow Articles by Ju, H.-J.
Right arrow Articles by Verchot-Lubicz, J.

The Potato Virus X TGBp2 Movement Protein Associates with Endoplasmic Reticulum-Derived Vesicles during Virus Infection1

Ho-Jong Ju, Timmy D. Samuels, Yuh-Shuh Wang, Elison Blancaflor, Mark Payton, Ruchira Mitra2, Konduru Krishnamurthy3, Richard S. Nelson and Jeanmarie Verchot-Lubicz*

Department of Entomology and Plant Pathology (H.-J.J., T.D.S., R.M., K.K., J.V.-L.), and Department of Statistics (M.P.), Oklahoma State University, Stillwater, Oklahoma 74078; and Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (Y.-S.W., E.B., R.S.N.)

The green fluorescent protein (GFP) gene was fused to the potato virus X (PVX) TGBp2 gene, inserted into either the PVX infectious clone or pRTL2 plasmids, and used to study protein subcellular targeting. In protoplasts and plants inoculated with PVX-GFP:TGBp2 or transfected with pRTL2-GFP:TGBp2, fluorescence was mainly in vesicles and the endoplasmic reticulum (ER). During late stages of virus infection, fluorescence became increasingly cytosolic and nuclear. Protoplasts transfected with PVX-GFP:TGBp2 or pRTL2-GFP:TGBp2 were treated with cycloheximide and the decline of GFP fluorescence was greater in virus-infected protoplasts than in pRTL2-GFP:TGBp2-transfected protoplasts. Thus, protein instability is enhanced in virus-infected protoplasts, which may account for the cytosolic and nuclear fluorescence during late stages of infection. Immunogold labeling and electron microscopy were used to further characterize the GFP:TGBp2-induced vesicles. Label was associated with the ER and vesicles, but not the Golgi apparatus. The TGBp2-induced vesicles appeared to be ER derived. For comparison, plasmids expressing GFP fused to TGBp3 were transfected to protoplasts, bombarded to tobacco leaves, and studied in transgenic leaves. The GFP:TGBp3 proteins were associated mainly with the ER and did not cause obvious changes in the endomembrane architecture, suggesting that the vesicles reported in GFP:TGBp2 studies were induced by the PVX TGBp2 protein. In double-labeling studies using confocal microscopy, fluorescence was associated with actin filaments, but not with Golgi vesicles. We propose a model in which reorganization of the ER and increased protein degradation is linked to plasmodesmata gating.

1 This work was supported by the Noble Foundation, the National Science Foundation (NSF) Integrative Plant Biology Program Award (IBM–9982552), the NSF Multi-user Instrumentation award (DBI–0400580), and the Oklahoma Agriculture Experiment Station (project H–2371).

2 Present address: Department of Plant and Soil Science, Delaware Biotechnology Institute, Newark, DE 19711.

3 Present address: Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.066019.

* Corresponding author; e-mail verchot{at}okstate.edu; fax 405–744–6039.

Received May 23, 2005; returned for revision June 8, 2005; accepted June 8, 2005.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
P. A. Harries, J.-W. Park, N. Sasaki, K. D. Ballard, A. J. Maule, and R. S. Nelson
Differing requirements for actin and myosin by plant viruses for sustained intercellular movement
PNAS, October 13, 2009; 106(41): 17594 - 17599.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H.-S. Lim, J. N. Bragg, U. Ganesan, S. Ruzin, D. Schichnes, M. Y. Lee, A. M. Vaira, K. H. Ryu, J. Hammond, and A. O. Jackson
Subcellular Localization of the Barley Stripe Mosaic Virus Triple Gene Block Proteins
J. Virol., September 15, 2009; 83(18): 9432 - 9448.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Hofmann, A. Niehl, A. Sambade, A. Steinmetz, and M. Heinlein
Inhibition of Tobacco Mosaic Virus Movement by Expression of an Actin-Binding Protein
Plant Physiology, April 1, 2009; 149(4): 1810 - 1823.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. A. Harries, K. Palanichelvam, W. Yu, J. E. Schoelz, and R. S. Nelson
The Cauliflower Mosaic Virus Protein P6 Forms Motile Inclusions That Traffic along Actin Microfilaments and Stabilize Microtubules
Plant Physiology, February 1, 2009; 149(2): 1005 - 1016.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. Wei and A. Wang
Biogenesis of Cytoplasmic Membranous Vesicles for Plant Potyvirus Replication Occurs at Endoplasmic Reticulum Exit Sites in a COPI- and COPII-Dependent Manner
J. Virol., December 15, 2008; 82(24): 12252 - 12264.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. V. Schepetilnikov, A. G. Solovyev, E. N. Gorshkova, J. Schiemann, A. I. Prokhnevsky, V. V. Dolja, and S. Y. Morozov
Intracellular Targeting of a Hordeiviral Membrane-Spanning Movement Protein: Sequence Requirements and Involvement of an Unconventional Mechanism
J. Virol., February 1, 2008; 82(3): 1284 - 1293.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
J. Verchot-Lubicz, C.-M. Ye, and D. Bamunusinghe
Molecular biology of potexviruses: recent advances
J. Gen. Virol., June 1, 2007; 88(6): 1643 - 1655.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H.-J. Ju, J. E. Brown, C.-M. Ye, and J. Verchot-Lubicz
Mutations in the Central Domain of Potato Virus X TGBp2 Eliminate Granular Vesicles and Virus Cell-to-Cell Trafficking
J. Virol., February 15, 2007; 81(4): 1899 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
O. V. Karpova, O. V. Zayakina, M. V. Arkhipenko, E. V. Sheval, O. I. Kiselyova, V. Yu. Poljakov, I. V. Yaminsky, N. P. Rodionova, and J. G. Atabekov
Potato virus X RNA-mediated assembly of single-tailed ternary 'coat protein-RNA-movement protein' complexes
J. Gen. Virol., September 1, 2006; 87(9): 2731 - 2740.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Ferralli, J. Ashby, M. Fasler, V. Boyko, and M. Heinlein
Disruption of Microtubule Organization and Centrosome Function by Expression of Tobacco Mosaic Virus Movement Protein.
J. Virol., June 1, 2006; 80(12): 5807 - 5821.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
M.-K. Lin, C.-C. Hu, N.-S. Lin, B.-Y. Chang, and Y.-H. Hsu
Movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system.
J. Gen. Virol., May 1, 2006; 87(Pt 5): 1357 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. S. Nelson and V. Citovsky
Plant Viruses. Invaders of Cells and Pirates of Cellular Pathways
Plant Physiology, August 1, 2005; 138(4): 1809 - 1814.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
J.-Z. Liu, E. B. Blancaflor, and R. S. Nelson
The Tobacco Mosaic Virus 126-Kilodalton Protein, a Constituent of the Virus Replication Complex, Alone or within the Complex Aligns with and Traffics along Microfilaments
Plant Physiology, August 1, 2005; 138(4): 1853 - 1865.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists