First published online July 8, 2005; 10.1104/pp.105.063115
Plant Physiology 138:2087-2096 (2005)
© 2005 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
OsPTF1, a Novel Transcription Factor Involved in Tolerance to Phosphate Starvation in Rice1,[w]
Keke Yi2,
Zhongchang Wu2,
Jie Zhou,
Liming Du,
Longbiao Guo,
Yunrong Wu and
Ping Wu*
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310029, People's Republic of China (K.Y., Z.W., J.Z., L.D., Y.W., P.W.); and China National Rice Research Institute, Hangzhou 310006, People's Republic of China (L.G.)
We report here on a novel transcription factor with a basic helix-loop-helix domain for tolerance to inorganic phosphate (Pi) starvation in rice (Oryza sativa). The gene is designated OsPTF1. The expression of OsPTF1 is Pi starvation induced in roots while constitutively expressed in shoots, as shown by northern-blot analysis. Overexpression of OsPTF1 enhanced tolerance to Pi starvation in transgenic rice. Tillering ability, root and shoot biomass, and phosphorus content of transgenic rice plants were about 30% higher than those of the wild-type plants in Pi-deficient conditions in hydroponic experiments. In soil pot and field experiments, more than 20% increase in tiller number, panicle weight, and phosphorus content was observed in transgenic plants compared to wild-type plants at low-Pi levels. In Pi-deficient conditions, transgenic rice plants showed significantly higher total root length and root surface area, which results in a higher instantaneous Pi uptake rate over their wild-type counterparts. Microarray analysis for transgenic plants overexpressing OsPTF1 has been performed to investigate the downstream regulation of OsPTF1.
1 This work was supported by the Key Basic Research Special Foundation of China (G1999011700), the Special Program of Rice Functional Genomics of China (2002AAZZ1003), the National Education Ministry of China, and the Science and Technology Bureau of Zhejiang Province.
2 These authors contributed equally to the paper.
[w] The online version of this article contains Web-only data.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.063115.
* Corresponding author; e-mail clspwu{at}zju.edu.cn; fax 86–571–86971323.
Received March 18, 2005;
returned for revision May 1, 2005;
accepted May 11, 2005.
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