This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 138/4/2374    most recent pp.105.060368v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (31) Citing Articles via Google Scholar Google Scholar Articles by Lidder, P. Articles by Green, P. J. Search for Related Content PubMed PubMed Citation Articles by Lidder, P. Articles by Green, P. J. Agricola Articles by Lidder, P. Articles by Green, P. J.

GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Circadian Control of Messenger RNA Stability. Association with a Sequence-Specific Messenger RNA Decay Pathway^1,[w]

Michigan State University-Department of Energy Plant Research Laboratory, Cell and Molecular Biology (P.L.), and Biochemistry and Molecular Biology (R.A.G.), Michigan State University, East Lansing, Michigan 48824; Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711 (P.L., P.J.G.); and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755 (P.A.S., C.R.M.)

Transcriptional and posttranscriptional regulation are well-established mechanisms for circadian gene expression. Among the latter, differential messenger RNA (mRNA) stability has been hypothesized to control gene expression in response to the clock. However, direct proof that the rate of mRNA turnover can be regulated by the clock is lacking. Previous microarray expression data for unstable mRNAs in Arabidopsis (Arabidopsis thaliana) revealed that mRNA instability is associated with a group of genes controlled by the circadian clock. Here, we show that CCR-LIKE (CCL) and SENESCENCE ASSOCIATED GENE 1 transcripts are differentially regulated at the level of mRNA stability at different times of day. In addition, the changes in CCL mRNA stability continue under free-running conditions, indicating that it is controlled by the Arabidopsis circadian clock. Furthermore, we show that these mRNAs are targets of the mRNA degradation pathway mediated by the downstream (DST) instability determinant. Disruption of the DST-mediated decay pathway in the dst1 mutant leads to aberrant circadian mRNA oscillations that correlate with alterations of the half-life of CCL mRNA relative to parental plants in the morning and afternoon. That this is due to an effect on the circadian control is evidenced by mRNA decay experiments carried out in continuous light. Finally, we show that the defects exhibited by dst mutants are reflected by an impact on circadian regulation at the whole plant level. Together, these results demonstrate that regulation of mRNA stability is important for clock-controlled expression of specific genes in Arabidopsis. Moreover, these data uncover a connection between circadian rhythms and a sequence-specific mRNA decay pathway.

² These authors contributed equally to the paper.

³ Present address: Department of Biology, New York University, 100 Washington Square East, 1009 Main Building, New York, NY 10003.

^[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.060368.

^* Corresponding author; e-mail green{at}dbi.udel.edu; fax 302–831–3231.

Received January 27, 2005; returned for revision May 5, 2005; accepted May 6, 2005.

This article has been cited by other articles:

				S. Jacobshagen, B. Kessler, and C. A. Rinehart At Least Four Distinct Circadian Regulatory Mechanisms Are Required for All Phases of Rhythms in mRNA Amount J Biol Rhythms, December 1, 2008; 23(6): 511 - 524. [Abstract] [PDF]

				Z. Bieniawska, C. Espinoza, A. Schlereth, R. Sulpice, D. K. Hincha, and M. A. Hannah Disruption of the Arabidopsis Circadian Clock Is Responsible for Extensive Variation in the Cold-Responsive Transcriptome Plant Physiology, May 1, 2008; 147(1): 263 - 279. [Abstract] [Full Text] [PDF]

				R. J. Ulbricht and W. M. Olivas Puf1p acts in combination with other yeast Puf proteins to control mRNA stability RNA, February 1, 2008; 14(2): 246 - 262. [Abstract] [Full Text] [PDF]

				R. Narsai, K. A. Howell, A. H. Millar, N. O'Toole, I. Small, and J. Whelan Genome-Wide Analysis of mRNA Decay Rates and Their Determinants in Arabidopsis thaliana PLANT CELL, November 1, 2007; 19(11): 3418 - 3436. [Abstract] [Full Text] [PDF]

				E. Yakir, D. Hilman, M. Hassidim, and R. M. Green CIRCADIAN CLOCK ASSOCIATED1 Transcript Stability and the Entrainment of the Circadian Clock in Arabidopsis Plant Physiology, November 1, 2007; 145(3): 925 - 932. [Abstract] [Full Text] [PDF]

				C. B. Green, N. Douris, S. Kojima, C. A. Strayer, J. Fogerty, D. Lourim, S. R. Keller, and J. C. Besharse From the Cover: Loss of Nocturnin, a circadian deadenylase, confers resistance to hepatic steatosis and diet-induced obesity PNAS, June 5, 2007; 104(23): 9888 - 9893. [Abstract] [Full Text] [PDF]

				E. Garbarino-Pico and C. B. Green Posttranscriptional Regulation of Mammalian Circadian Clock Output Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 145 - 156. [Abstract] [PDF]

				J. D. Keene Biological Clocks and the Coordination Theory of RNA Operons and Regulons Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 157 - 165. [Abstract] [PDF]

				D. Benjamin, M. Schmidlin, L. Min, B. Gross, and C. Moroni BRF1 Protein Turnover and mRNA Decay Activity Are Regulated by Protein Kinase B at the Same Phosphorylation Sites Mol. Cell. Biol., December 15, 2006; 26(24): 9497 - 9507. [Abstract] [Full Text] [PDF]