First published online August 26, 2005; 10.1104/pp.105.062372
Plant Physiology 139:341-352 (2005)
© 2005 American Society of Plant Biologists
PLANTS INTERACTING WITH OTHER ORGANISMS
NpPDR1, a Pleiotropic Drug Resistance-Type ATP-Binding Cassette Transporter from Nicotiana plumbaginifolia, Plays a Major Role in Plant Pathogen Defense1
Yvan Stukkens,
Alain Bultreys,
Sébastien Grec,
Tomasz Trombik,
Delphine Vanham and
Marc Boutry*
Unité de Biochimie Physiologique, Institut des Sciences de la Vie, Université Catholique de Louvain, B–1348 Louvain-la-Neuve, Belgium (Y.S., S.G., T.T., D.V., M.B.); and Département Biotechnologie, Centre Wallon de Recherches Agronomiques, B–5030 Gembloux, Belgium (A.B.)
Nicotiana plumbaginifolia NpPDR1, a plasma membrane pleiotropic drug resistance-type ATP-binding cassette transporter formerly named NpABC1, has been suggested to transport the diterpene sclareol, an antifungal compound. However, direct evidence for a role of pleiotropic drug resistance transporters in the plant defense is still lacking. In situ immunolocalization and histochemical analysis using the gusA reporter gene showed that NpPDR1 was constitutively expressed in the whole root, in the leaf glandular trichomes, and in the flower petals. However, NpPDR1 expression was induced in the whole leaf following infection with the fungus Botrytis cinerea, and the bacteria Pseudomonas syringae pv tabaci, Pseudomonas fluorescens, and Pseudomonas marginalis pv marginalis, which do not induce a hypersensitive response in N. plumbaginifolia, whereas a weaker response was observed using P. syringae pv syringae, which does induce a hypersensitive response. Induced NpPDR1 expression was more associated with the jasmonic acid than the salicylic acid signaling pathway. These data suggest that NpPDR1 is involved in both constitutive and jasmonic acid-dependent induced defense. Transgenic plants in which NpPDR1 expression was prevented by RNA interference showed increased sensitivity to sclareol and reduced resistance to B. cinerea. These data show that NpPDR1 is involved in pathogen resistance and thus demonstrate a new role for the ATP-binding cassette transporter family.
1 This work was supported by grants from the Belgian National Fund for Scientific Research, the Interuniversity Attraction Poles Program-Belgian Science Policy, and the European Community (IHP-RTN). Y.S. was a recipient of a fellowship from the Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture, and A.B. is funded by the Ministère de la Région Wallonne.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.062372.
* Corresponding author; e-mail boutry{at}fysa.ucl.ac.be; fax (32)–10–47–38–72.
Received March 18, 2005;
returned for revision April 22, 2005;
accepted July 13, 2005.
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