First published online August 26, 2005; 10.1104/pp.105.065565
Plant Physiology 139:466-473 (2005)
© 2005 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
Effects of Water Stress on Respiration in Soybean Leaves1
Miquel Ribas-Carbo*,
Nicolas L. Taylor,
Larry Giles,
Silvia Busquets,
Patrick M. Finnegan,
David A. Day2,
Hans Lambers,
Hipólito Medrano,
Joseph A. Berry and
Jaume Flexas
Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Universitat de les Illes Balears, 07122 Illes Balears, Spain (M.R.-C., H.M., J.F.); Biochemistry and Molecular Biology, School of Biomedical and Chemical Sciences (N.L.T., D.A.D.), and School of Plant Biology (N.L.T., P.M.F., H.L.), University of Western Australia, Crawley, Western Australia 6009, Australia; Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305 (L.G., J.A.B.); and Departament de Bioquímica y Biologia Molecular, Universitat de Barcelona, 08028 Barcelona, Spain (S.B.)
The effect of water stress on respiration and mitochondrial electron transport has been studied in soybean (Glycine max) leaves, using the oxygen-isotope-fractionation technique. Treatments with three levels of water stress were applied by irrigation to replace 100%, 50%, and 0% of daily water use by transpiration. The levels of water stress were characterized in terms of light-saturated stomatal conductance (gs): well irrigated (gs > 0.2 mol H2O m–2 s–1), mildly water stressed (gs between 0.1 and 0.2 mol H2O m–2 s–1), and severely water stressed (gs < 0.1 mol H2O m–2 s–1). Although net photosynthesis decreased by 40% and 70% under mild and severe water stress, respectively, the total respiratory oxygen uptake (Vt) was not significantly different at any water-stress level. However, severe water stress caused a significant shift of electrons from the cytochrome to the alternative pathway. The electron partitioning through the alternative pathway increased from 10% to 12% under well-watered or mild water-stress conditions to near 40% under severe water stress. Consequently, the calculated rate of mitochondrial ATP synthesis decreased by 32% under severe water stress. Unlike many other stresses, water stress did not affect the levels of mitochondrial alternative oxidase protein. This suggests a biochemical regulation (other than protein synthesis) that causes this mitochondrial electron shift.
1 This work was supported by the Spanish Ministry of Science and Technology (MCyT grant no. BFI2002–00772) and the Australian Research Council. This is Carnegie Institution of Washington Department of Global Ecology publication number 96.
2 Present address: Faculty of Science, University of Sydney, Sydney, NSW 2006, Australia.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.065565.
* Corresponding author; e-mail mribas{at}uib.es; fax 34–971–173184.
Received May 12, 2005;
returned for revision June 20, 2005;
accepted June 20, 2005.
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