First published online September 23, 2005; 10.1104/pp.105.066233
Plant Physiology 139:979-990 (2005)
© 2005 American Society of Plant Biologists
BIOENERGETICS AND PHOTOSYNTHESIS
The Regulation of Rubisco Activity in Response to Variation in Temperature and Atmospheric CO2 Partial Pressure in Sweet Potato1,[w]
Yan-Ping Cen2 and
Rowan F. Sage*
Department of Botany, University of Toronto, Toronto, Ontario, Canada M5S 3B2
The temperature response of net CO2 assimilation rate (A), the rate of whole-chain electron transport, the activity and activation state of Rubisco, and the pool sizes of ribulose-1,5-bisphosphate (RuBP) and 3-phosphoglyceric acid (PGA) were assessed in sweet potato (Ipomoea batatas) grown under greenhouse conditions. Above the thermal optimum of photosynthesis, the activation state of Rubisco declined with increasing temperature. Doubling CO2 above 370 µbar further reduced the activation state, while reducing CO2 by one-half increased it. At cool temperature (<16°C), the activation state of Rubisco declined at CO2 levels where photosynthesis was unaffected by a 90% reduction in O2 content. Reduction of the partial pressure of CO2 at cool temperature also enhanced the activation state of Rubisco. The rate of electron transport showed a pronounced temperature response with the same temperature optimum as A at elevated CO2. RuBP pool size and the RuBP-to-PGA ratio declined with increasing temperature. Increasing CO2 also reduced the RuBP pool size. These results are consistent with the hypothesis that the reduction in the activation state of Rubisco at high and low temperature is a regulated response to a limitation in one of the processes contributing to the rate of RuBP regeneration. To further evaluate this possibility, we used measured estimates of Rubisco capacity, electron transport capacity, and the inorganic phosphate regeneration capacity to model the response of A to temperature. At elevated CO2, the activation state of Rubisco declined at high temperatures where electron transport capacity was predicted to be limiting, and at cooler temperatures where the inorganic phosphate regeneration capacity was limiting. At low CO2, where Rubisco capacity was predicted to limit photosynthesis, full activation of Rubisco was observed at all measurement temperatures.
1 This work was supported by the National Sciences and Engineering Research Council of Canada (grant no. OGP0154273).
2 Present address: Biology Department, Queens University, Kingston, Ontario, Canada K7L 3N6.
[w] The online version of this article contains Web-only data.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.066233.
* Corresponding author; e-mail rsage{at}botany.utoronto.ca; fax 14169785878.
Received May 26, 2005;
returned for revision August 6, 2005;
accepted August 9, 2005.
This article has been cited by other articles:

|
 |

|
 |
 
M. I. Hozain, M. E. Salvucci, M. Fokar, and A. S. Holaday
The differential response of photosynthesis to high temperature for a boreal and temperate Populus species relates to differences in Rubisco activation and Rubisco activase properties
Tree Physiol,
October 28, 2009;
(2009)
tpp091v1.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. P. Scafaro, P. A. Haynes, and B. J. Atwell
Physiological and molecular changes in Oryza meridionalis Ng., a heat-tolerant species of wild rice
J. Exp. Bot.,
October 9, 2009;
(2009)
erp294v1.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Eichelmann, E. Talts, V. Oja, E. Padu, and A. Laisk
Rubisco in planta kcat is regulated in balance with photosynthetic electron transport
J. Exp. Bot.,
October 1, 2009;
60(14):
4077 - 4088.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. D. B. Leakey, E. A. Ainsworth, C. J. Bernacchi, A. Rogers, S. P. Long, and D. R. Ort
Elevated CO2 effects on plant carbon, nitrogen, and water relations: six important lessons from FACE
J. Exp. Bot.,
July 1, 2009;
60(10):
2859 - 2876.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Nagai and A. Makino
Differences Between Rice and Wheat in Temperature Responses of Photosynthesis and Plant Growth
Plant Cell Physiol.,
April 1, 2009;
50(4):
744 - 755.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. F. Sage, D. A. Way, and D. S. Kubien
Rubisco, Rubisco activase, and global climate change
J. Exp. Bot.,
May 1, 2008;
59(7):
1581 - 1595.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. A. J. Parry, A. J. Keys, P. J. Madgwick, A. E. Carmo-Silva, and P. J. Andralojc
Rubisco regulation: a role for inhibitors
J. Exp. Bot.,
May 1, 2008;
59(7):
1569 - 1580.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Makino and R. F. Sage
Temperature Response of Photosynthesis in Transgenic Rice Transformed with 'Sense' or 'Antisense' rbcS
Plant Cell Physiol.,
October 1, 2007;
48(10):
1472 - 1483.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
O. Stasik and H. G. Jones
Response of photosynthetic apparatus to moderate high temperature in contrasting wheat cultivars at different oxygen concentrations
J. Exp. Bot.,
June 1, 2007;
58(8):
2133 - 2143.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P Priault, G Tcherkez, G Cornic, R De Paepe, R Naik, J Ghashghaie, and P Streb
The lack of mitochondrial complex I in a CMSII mutant of Nicotiana sylvestris increases photorespiration through an increased internal resistance to CO2 diffusion
J. Exp. Bot.,
September 1, 2006;
57(12):
3195 - 3207.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. F. Sage and A. D. McKown
Is C4 photosynthesis less phenotypically plastic than C3 photosynthesis?
J. Exp. Bot.,
January 1, 2006;
57(2):
303 - 317.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|
|