First published online October 28, 2005; 10.1104/pp.105.067553
Plant Physiology 139:1495-1506 (2005)
© 2005 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS
HKT1 Mediates Sodium Uniport in Roots. Pitfalls in the Expression of HKT1 in Yeast1
Rosario Haro2,
María A. Bañuelos2,
María E. Senn,
Javier Barrero-Gil and
Alonso Rodríguez-Navarro*
Departamento de Biotecnología, Universidad Politécnica de Madrid, 28040 Madrid, Spain
The function of HKT1 in roots is controversial. We tackled this controversy by studying Na+ uptake in barley (Hordeum vulgare) roots, cloning the HvHKT1 gene, and expressing the HvHKT1 cDNA in yeast (Saccharomyces cerevisiae) cells. High-affinity Na+ uptake was not detected in plants growing at high K+ but appeared soon after exposing the plants to a K+-free medium. It was a uniport, insensitive to external K+ at the beginning of K+ starvation and inhibitable by K+ several hours later. The expression of HvHKT1 in yeast was Na+ (or K+) uniport, Na+-K+ symport, or a mix of both, depending on the construct from which the transporter was expressed. The Na+ uniport function was insensitive to external K+ and mimicked the Na+ uptake carried out by the roots at the beginning of K+ starvation. The K+ uniport function only took place in yeast cells that were completely K+ starved and disappeared when internal K+ increased, which makes it unlikely that HvHKT1 mediates K+ uptake in roots. Mutation of the first in-frame AUG codon of HvHKT1 to CUC changed the uniport function into symport. The expression of the symport from either mutants or constructs keeping the first in-frame AUG took place only in K+-starved cells, while the uniport was expressed in all conditions. We discuss here that the symport occurs only in heterologous expression. It is most likely related to the K+ inhibitable Na+ uptake process of roots that heterologous systems fail to reproduce.
1 This work was supported by the government of Spain (grant no. AGL2004–05153), Ramón y Cajal fellowship (to M.A.B.), Doctoral Fellowship (to J.B.); by the government of Argentina, CONICET Doctoral Fellowship (to M.E.S.); and by the European Regional Development Fund program of the European Union.
2 These authors contributed equally to the paper.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Alonso Rodríguez-Navarro (alonso.rodriguez{at}upm.es).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.067553.
* Corresponding author; e-mail alonso.rodriguez{at}upm.es; fax 34–913365757.
Received June 22, 2005;
returned for revision July 13, 2005;
accepted July 13, 2005.
This article has been cited by other articles:
|
|
|
|
 
H. J. Kronzucker, M. W. Szczerba, L. M. Schulze, and D. T. Britto
Non-reciprocal interactions between K+ and Na+ ions in barley (Hordeum vulgare L.)
J. Exp. Bot.,
July 1, 2008;
59(10):
2793 - 2801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Banuelos, R. Haro, A. Fraile-Escanciano, and A. Rodriguez-Navarro
Effects of Polylinker uATGs on the Function of Grass HKT1 Transporters Expressed in Yeast Cells
Plant Cell Physiol.,
July 1, 2008;
49(7):
1128 - 1132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Huang, W. Spielmeyer, E. S. Lagudah, and R. Munns
Comparative mapping of HKT genes in wheat, barley, and rice, key determinants of Na+ transport, and salt tolerance
J. Exp. Bot.,
March 5, 2008;
(2008)
ern033v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-M. Wang, J.-L. Zhang, and T. J. Flowers
Low-Affinity Na+ Uptake in the Halophyte Suaeda maritima
Plant Physiology,
October 1, 2007;
145(2):
559 - 571.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Grabov
Plant KT/KUP/HAK Potassium Transporters: Single Family - Multiple Functions
Ann. Bot.,
June 1, 2007;
99(6):
1035 - 1041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Byrt, J. D. Platten, W. Spielmeyer, R. A. James, E. S. Lagudah, E. S. Dennis, M. Tester, and R. Munns
HKT1;5-Like Cation Transporters Linked to Na+ Exclusion Loci in Wheat, Nax2 and Kna1
Plant Physiology,
April 1, 2007;
143(4):
1918 - 1928.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. James, R. J. Davenport, and R. Munns
Physiological Characterization of Two Genes for Na+ Exclusion in Durum Wheat, Nax1 and Nax2
Plant Physiology,
December 1, 2006;
142(4):
1537 - 1547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Huang, W. Spielmeyer, E. S. Lagudah, R. A. James, J. D. Platten, E. S. Dennis, and R. Munns
A Sodium Transporter (HKT7) Is a Candidate for Nax1, a Gene for Salt Tolerance in Durum Wheat
Plant Physiology,
December 1, 2006;
142(4):
1718 - 1727.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Rodriguez-Navarro and F. Rubio
High-affinity potassium and sodium transport systems in plants
J. Exp. Bot.,
March 1, 2006;
57(5):
1149 - 1160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Pardo, B. Cubero, E. O. Leidi, and F. J. Quintero
Alkali cation exchangers: roles in cellular homeostasis and stress tolerance
J. Exp. Bot.,
March 1, 2006;
57(5):
1181 - 1199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
