This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 139/4/1677    most recent pp.105.067140v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Lin, W.-H. Articles by Xue, H.-W. Search for Related Content PubMed PubMed Citation Articles by Lin, W.-H. Articles by Xue, H.-W. Agricola Articles by Lin, W.-H. Articles by Xue, H.-W.

CELL BIOLOGY AND SIGNAL TRANSDUCTION

At5PTase13 Modulates Cotyledon Vein Development through Regulating Auxin Homeostasis^1,[W]

National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, People's Republic of China (W.-H.L., Y.W., Z.-H.X., H.-W.X.); Partner Group of the Max-Planck-Institute of Molecular Plant Physiology on Plant Molecular Physiology and Signal Transduction, 200032 Shanghai, People's Republic of China (W.-H.L., Y.W., H.-W.X.); University of Potsdam, Institute of Biochemistry and Biology, D–14476 Golm, Germany (B.M.-R.); and School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom (C.A.B.)

Phosphatidylinositol signaling pathway and the relevant metabolites are known to be critical to the modulation of different aspects of plant growth, development, and stress responses. Inositol polyphosphate 5-phosphatase is a key enzyme involved in phosphatidylinositol metabolism and is encoded by an At5PTase gene family in Arabidopsis thaliana. A previous study shows that At5PTase11 mediates cotyledon vascular development probably through the regulation of intracellular calcium levels. In this study, we provide evidence that At5PTase13 modulates the development of cotyledon veins through its regulation of auxin homeostasis. A T-DNA insertional knockout mutant, At5pt13-1, showed a defect in development of the cotyledon vein, which was rescued completely by exogenous auxin and in part by brassinolide, a steroid hormone. Furthermore, the mutant had reduced auxin content and altered auxin accumulation in seedlings revealed by the DR5:-glucuronidase fusion construct in seedlings. In addition, microarray analysis shows that the transcription of key genes responsible for auxin biosynthesis and transport was altered in At5pt13-1. The At5pt13-1 mutant was also less sensitive to auxin inhibition of root elongation. These results suggest that At5PTase13 regulates the homeostasis of auxin, a key hormone controlling vascular development in plants.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Hong-Wei Xue (hwxue{at}sibs.ac.cn).

^[W] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.067140.

^* Corresponding author; e-mail hwxue{at}sibs.ac.cn; fax 86–21–54924060.

Received June 14, 2005; returned for revision July 22, 2005; accepted August 24, 2005.

This article has been cited by other articles:

				X. Chen, W.-H. Lin, Y. Wang, S. Luan, and H.-W. Xue An Inositol Polyphosphate 5-Phosphatase Functions in PHOTOTROPIN1 Signaling in Arabidopis by Altering Cytosolic Ca2+ PLANT CELL, February 1, 2008; 20(2): 353 - 366. [Abstract] [Full Text] [PDF]

				I. Y. Perera, C.-Y. Hung, S. Brady, G. K. Muday, and W. F. Boss A Universal Role for Inositol 1,4,5-Trisphosphate-Mediated Signaling in Plant Gravitropism Plant Physiology, February 1, 2006; 140(2): 746 - 760. [Abstract] [Full Text] [PDF]