This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 139/4/1692    most recent pp.105.069765v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Wang, Q. Articles by Baluska, F. Search for Related Content PubMed PubMed Citation Articles by Wang, Q. Articles by Baluska, F. Agricola Articles by Wang, Q. Articles by Baluska, F.

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Effects of Brefeldin A on Pollen Germination and Tube Growth. Antagonistic Effects on Endocytosis and Secretion^1,[W]

Qinli Wang, Lingan Kong, Huaiqing Hao, Xiaohua Wang, Jinxing Lin^*, Jozef amaj and Frantiek Baluka

Key Laboratory of Photosynthesis and Molecular Environment Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Q.W., L.K., H.H., X.W., J.L.); Graduate School of the Chinese Academy of Sciences, Beijing 100049, China (Q.W., X.W.); Institute of Cellular and Molecular Botany, Department of Plant Cell Biology, Rheinische Friedrich-Wilhelms-University Bonn, D–53115 Bonn, Germany (J.S., F.B.); Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, SK–95007, Nitra, Slovak Republic (J.S); and Institute of Botany, Slovak Academy of Sciences, SK–84223, Bratislava, Slovak Republic (F.B.)

We assessed the effects of brefeldin A (BFA) on pollen tube development in Picea meyeri using fluorescent marker FM4-64 as a membrane-inserted endocytic/recycling marker, together with ultrastructural studies and Fourier transform infrared analysis of cell walls. BFA inhibited pollen germination and pollen tube growth, causing morphological changes in a dose-dependent manner, and pollen tube tip growth recovered after transferring into BFA-free medium. FM4-64 labeling showed typical bright apical staining in normally growing P. meyeri pollen tubes; this apical staining pattern differed from the V-formation pattern found in angiosperm pollen tubes. Confocal microscopy revealed that exocytosis was greatly inhibited in the presence of BFA. In contrast, the overall uptake of FM4-64 dye was about 2-fold that in the control after BFA (5 µg mL^–1) treatment, revealing that BFA stimulated endocytosis in a manner opposite to the induced changes in exocytosis. Transmission electron microscopic observation showed that the number of secretory vesicles at the apical zone dramatically decreased, together with the disappearance of paramural bodies, while the number of vacuoles and other larger organelles increased. An acid phosphatase assay confirmed that the addition of BFA significantly inhibited secretory pathways. Importantly, Fourier transform infrared microspectroscopy documented significant changes in the cell wall composition of pollen tubes growing in the presence of BFA. These results suggest that enhanced endocytosis, together with inhibited secretion, is responsible for the retarded growth of pollen tubes induced by BFA.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Jinxing Lin (linjx{at}ibcas.ac.cn).

^[W] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.069765.

^* Corresponding author; e-mail linjx{at}ibcas.ac.cn; fax 0086–10–62590833.

Received August 10, 2005; returned for revision August 10, 2005; accepted September 2, 2005.

This article has been cited by other articles:

				G. Lycett The role of Rab GTPases in cell wall metabolism J. Exp. Bot., November 1, 2008; 59(15): 4061 - 4074. [Abstract] [Full Text] [PDF]

				T. Goh, W. Uchida, S. Arakawa, E. Ito, T. Dainobu, K. Ebine, M. Takeuchi, K. Sato, T. Ueda, and A. Nakano VPS9a, the Common Activator for Two Distinct Types of Rab5 GTPases, Is Essential for the Development of Arabidopsis thaliana PLANT CELL, November 1, 2007; 19(11): 3504 - 3515. [Abstract] [Full Text] [PDF]

				S. Romagnoli, G. Cai, C. Faleri, E. Yokota, T. Shimmen, and M. Cresti Microtubule- and Actin Filament-Dependent Motors are Distributed on Pollen Tube Mitochondria and Contribute Differently to Their Movement Plant Cell Physiol., February 1, 2007; 48(2): 345 - 361. [Abstract] [Full Text] [PDF]

				P. Campanoni and M. R. Blatt Membrane trafficking and polar growth in root hairs and pollen tubes J. Exp. Bot., January 1, 2007; 58(1): 65 - 74. [Abstract] [Full Text] [PDF]

				T. Chen, N. Teng, X. Wu, Y. Wang, W. Tang, J. Samaj, F. Baluska, and J. Lin Disruption of Actin Filaments by Latrunculin B Affects Cell Wall Construction in Picea meyeri Pollen Tube by Disturbing Vesicle Trafficking Plant Cell Physiol., January 1, 2007; 48(1): 19 - 30. [Abstract] [Full Text] [PDF]

				X. Sheng, Z. Hu, H. Lu, X. Wang, F. Baluska, J. Samaj, and J. Lin Roles of the Ubiquitin/Proteasome Pathway in Pollen Tube Growth with Emphasis on MG132-Induced Alterations in Ultrastructure, Cytoskeleton, and Cell Wall Components Plant Physiology, August 1, 2006; 141(4): 1578 - 1590. [Abstract] [Full Text] [PDF]

				X. Wang, Y. Teng, Q. Wang, X. Li, X. Sheng, M. Zheng, J. Samaj, F. Baluska, and J. Lin Imaging of Dynamic Secretory Vesicles in Living Pollen Tubes of Picea meyeri Using Evanescent Wave Microscopy Plant Physiology, August 1, 2006; 141(4): 1591 - 1603. [Abstract] [Full Text] [PDF]